Progesterone induces expression of endometrial messenger RNA encoding for cyclooxygenase (sheep)

Eggleston, D.L.; Wilken, C.; Kirk, Edward A. Van; Slaughter, R.G.; Ji, Tae H.; Murdoch, William J.

doi:10.1016/0090-6980(90)90027-s

Cited by 75 publications

(18 citation statements)

References 16 publications

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“…In both studies, HSD11B1 mRNA and PTGS2 protein were absent from the endometrial epithelia of ewes treated with RU486. Stimulation of PTGS2 expression by P4 in ovine uteri was reported previously [20,29]. Although postulated previously [16], the present studies are the first to demonstrate that P4 induces HSD11B1 expression in uterine LE/sGE.…”

Section: Discussionmentioning

confidence: 49%

HSD11B1, HSD11B2, PTGS2, and NR3C1 Expression in the Peri-Implantation Ovine Uterus: Effects of Pregnancy, Progesterone, and Interferon Tau1

Simmons

Satterfield

Welsh

et al. 2010

Biology of Reproduction

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Establishment of pregnancy in ruminants requires conceptus elongation and production of interferon tau (IFNT), the pregnancy recognition signal that maintains the corpus luteum and progesterone (P4) secretion. The enzymes hydroxysteroid (11-beta) dehydrogenase 1 (HSD11B1) and HSD11B2 catalyze the interconversion of inactive cortisone and active cortisol, which is a biologically active glucorticoid and ligand for the receptor subfamily 3, group C, member 1 (glucocorticoid receptor) (NR3C1). The activity of HSD11B1 is stimulated by P4, prostaglandins, and cortisol. These studies determined the effects of pregnancy, P4, and IFNT on HSD11B1, HSD11B2, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) (PTGS2), and nuclear NR3C1 in the ovine uterus. Endometrial HSD11B1 mRNA levels were more abundant between Days 12 and 16 of pregnancy than the estrous cycle, and HSD11B1 and PTGS2 expression in the endometrial luminal and superficial glandular epithelia was coincident with conceptus elongation. HSD11B1 mRNA was very low in the conceptus, whereas HSD11B2 mRNA was abundant in the conceptus but not in the uterus. Treatment of ewes with P4 induced, and intrauterine infusions of IFNT modestly stimulated, HSD11B1 expression in the endometrial luminal and superficial glandular epithelia. In all of the studies, HSD11B1 and PTGS2 expression was coincident in the endometrial epithelia, and NR3C1 was present in all endometrial cell types. Collectively, these results support hypotheses that endometrial epithelial HSD11B1 expression is induced by P4 as well as stimulated by IFNT and PTGS2-derived prostaglandins and that HSD11B1-regenerated cortisol acts via NR3C1 to regulate ovine endometrial functions during early pregnancy.

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Section: Discussionmentioning

confidence: 49%

HSD11B1, HSD11B2, PTGS2, and NR3C1 Expression in the Peri-Implantation Ovine Uterus: Effects of Pregnancy, Progesterone, and Interferon Tau1

Simmons

Satterfield

Welsh

et al. 2010

Biology of Reproduction

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“…Plasma progesterone concentration was determined by solidphase RIA (Diagnostics Products Corporation, Los Angeles, CA, USA) as described by Eggleston et al (1990). The antibody against progesterone had !2 .…”

Section: Progesterone Analysismentioning

confidence: 99%

Low blood ISG15 mRNA and progesterone levels are predictive of non-pregnant dairy cows

Han

Austin

Rempel

et al. 2006

Journal of Endocrinology

129

107

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ISG15 is induced by conceptus-derived interferon-t in the endometrium on days 15-45 of pregnancy. It was hypothesized that pregnancy induces blood cell ISG15 gene expression and that low blood ISG15 mRNA levels provide an indication of non-pregnant cows on day 18. Blood was collected either on day 18 (nZ78) or on days 15-21, 25, and 32 (nZ21; serial collection) from dairy cows following artificial insemination (AI). Plasma progesterone concentration was determined using RIA. ISG15 mRNA levels were determined using real-time PCR. Pregnancy was diagnosed on day 32 using transrectal ultrasound. ISG15 mRNA levels increased after day 16, peaked at day 20 and then declined to day 16 levels by 32 days following AI. The average pregnancy rate was 43% based on blood cell ISG15 mRNA. The average pregnancy rate was 33% based on the transrectal ultrasound. Lower levels of ISG15 mRNA or progesterone during serial collections were 100% accurate in predicting non-pregnant cows based on day 32 transrectal ultrasound. However, detection of ISG15 mRNA yielded 78% accuracy in predicting pregnant cows, while progesterone yielded 58% accuracy. Average plasma progesterone based on pregnancy status according to ultrasound was consistently higher in pregnant (O4 ng/ml) when compared with non-pregnant cows from days 15 to 32, except on day 16. It is concluded that detection of low blood ISG15 mRNA levels during serial collection from days 17 to 25 serves as an accurate indicator of cows that are not pregnant, thus allowing re-synchronization and insemination.

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“…Progesterone is a potent stimulator of nonpregnant sheep endometrial COX2 [23,29,30]. To our knowledge, the priming effect of progesterone on the uterine or cervical PG system in pregnant animals has not been determined in any species.…”

Section: Discussionmentioning

confidence: 92%

“…On the one hand, progesterone, acting as a gene suppressor, down-regulates a number of genes that are essential for myometrial contraction, including the gap junction protein [41,42], calcium channels, steroid receptor and associated proteins [40,43], and oxytocin receptor [41,44]. On the other hand, progesterone can also promote intrauterine PG production [23,29,30,45]. High progesterone concentrations are temporally associated with the development of intrauterine PG production in pregnant sheep and women [24,27].…”

Section: Discussionmentioning

confidence: 99%

Sufficient Progesterone-Priming Prior to Estradiol Stimulation Is Required for Optimal Induction of the Cervical Prostaglandin System in Pregnant Sheep at 0.7 Gestations1

Wen¹,

Coksaygan²,

Chakrabarty³

et al. 2005

Biology of Reproduction

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The purposes of this study were to determine the separate and interactive functions of progesterone and estradiol in regulating the cervical prostaglandin (PG) system in pregnant sheep at 0.7 gestations. At 106-108 days of gestational age (dGA), ewes were treated with vehicle for 14 days (n = 5) or vehicle for 12 days followed by estradiol 5 mg twice a day, intramuscularly for 2 days (n = 5) or progesterone 100 mg, twice a day, intramuscularly for 14 days (n = 5) or progesterone 100 mg twice a day, intramuscularly for 10 days and then 2 days vehicle followed by estradiol 5 mg twice a day intramuscularly for 2 days (n = 5). At 121-123 dGA, cervical tissues were obtained under halothane anesthesia. Cervical RNA and protein were extracted and analyzed for prostaglandin-endoperoxide synthase 2 (COX2), two PGE(2) receptors, PTGER2 and PTGER4, and estrogen receptor alpha (ESR1) by Northern and Western blot analysis. Immunocytochemistry and in situ hybridization were applied to localize cellular distribution of COX2, PTGER2, and PTGER4 in the cervix. Data were analyzed by ANOVA. COX2 and PTGER4 mRNAs and proteins were increased (P < 0.05) in ewes treated with combined estradiol and progesterone but not in ewes treated with estradiol or progesterone alone compared with controls. ESR1 mRNA was increased in ewes treated with progesterone and estradiol plus progesterone. In contrast, PTGER2 mRNA and protein remained the same after all treatments. COX2 mRNA and protein were localized only in cervical glandular epithelial cells, whereas PTGER2 and PTGER4 were localized in both cervical glandular epithelial and smooth muscle cells. In conclusion, these data suggest that additional progesterone priming at 0.7 gestations synergizes with estradiol to induce cervical COX2, PTGER4, and ESR1 and support our hypothesis that stimulation of the cervical PG system by estradiol is optimized by sufficient progesterone priming in the pregnant sheep cervix.

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Progesterone induces expression of endometrial messenger RNA encoding for cyclooxygenase (sheep)

Cited by 75 publications

References 16 publications

HSD11B1, HSD11B2, PTGS2, and NR3C1 Expression in the Peri-Implantation Ovine Uterus: Effects of Pregnancy, Progesterone, and Interferon Tau1

HSD11B1, HSD11B2, PTGS2, and NR3C1 Expression in the Peri-Implantation Ovine Uterus: Effects of Pregnancy, Progesterone, and Interferon Tau1

Low blood ISG15 mRNA and progesterone levels are predictive of non-pregnant dairy cows

Sufficient Progesterone-Priming Prior to Estradiol Stimulation Is Required for Optimal Induction of the Cervical Prostaglandin System in Pregnant Sheep at 0.7 Gestations1

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