Progesterone Induced Blocking Factor (PIBF) Mediates Progesterone Induced Suppression of Decidual Lymphocyte Cytotoxicity

Laškarin, Gordana; Tokmadžić, Vlatka Sotošek; Štrbo, Nataša; Bogović, Tatjana; Szekeres-Barthó, Júlia; Randić, Ljiljana; Podack, Eckhard R.; Rukavina, Daniel

doi:10.1034/j.1600-0897.2002.01133.x

Cited by 57 publications

(47 citation statements)

References 31 publications

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“…There is a significant loss of cytotoxic T lymphocytes (CTL) and NK cells in the FRT during the luteal phase [47,48]. Progesterone exerts a strong immunosuppressive effect by blocking CTL-mediated killing by inhibition of perforin expression [49]. Gene profiling studies of the endometrium revealed that there is more robust appearance of class II molecule upregulation (HLA-DPA1, DPB1, DQB1, and DRA), complement pathway (SERPING1, C1, C1q, C3A and C5A), secretoglobin as well as a variety of NK cell receptors in the late-secretory phase [50].…”

Section: Discussionmentioning

confidence: 99%

Transcriptional Profiling of Human Endocervical Tissues Reveals Distinct Gene Expression in the Follicular and Luteal Phases of the Menstrual Cycle1

Yildiz-Arslan

Coon

Hope

et al. 2016

Biology of Reproduction

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The endocervix plays an important role in providing appropriate protective mechanisms of the upper female reproductive tract (FRT) while at the same time providing the appropriate milieu for sperm transport. Hormone fluctuations throughout the menstrual cycle contribute to changes in the mucosal environment that render the FRT vulnerable to infectious diseases. The objective of this study was to identify genes in human endocervix tissues that were differentially expressed in the follicular versus the luteal phases of the menstrual cycle using gene expression profiling. A microarray using the IIlumina platform was performed with eight endocervix tissues from follicular and four tissues from luteal phases of the menstrual cycle. Data analysis revealed significant differential expression of 110 genes between the two phases, with a P value <0.05 and a fold change cutoff of 1.5. Categorization of these genes, using Ingenuity Pathway Analysis, MetaCore from Thomson Reuters, and DAVID, revealed genes associated with extracellular matrix remodeling and cell-matrix interactions, amino acid metabolism, and lipid metabolism, as well as immune regulation in the follicular phase tissues. In luteal phase tissues, genes associated with chromatin remodeling, inflammation, angiogenesis, oxidative stress, and immune cell regulation were predominately expressed. Using samples from additional patients' tissues, select genes were confirmed by quantitative real-time PCR; immunohistochemical staining was also done to examine protein levels. This is the first microarray analysis comparing gene expression in endocervix tissues in cycling women. This study identified key genes and molecular pathways that were differentially regulated during the menstrual cycle.

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Section: Discussionmentioning

confidence: 99%

Transcriptional Profiling of Human Endocervical Tissues Reveals Distinct Gene Expression in the Follicular and Luteal Phases of the Menstrual Cycle1

Yildiz-Arslan

Coon

Hope

et al. 2016

Biology of Reproduction

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“…From targets' prediction data, we found upregulated miRNAs (hsa-miR-519a-1 and hsa-miR-520h) in decidua and low expressed miRNA (hsa-miR-520f) in villus of RSA patients, all target to LIF (Supplementary Table 3, see section on supplementary data given at the end of this article), substantial evidence suggests that LIF contributes to reproductive failure (Choudhury & Knapp 2001). In decidua, PIBF is known as a target gene of hsa-miR 520h (Supplementary Table 3), and PIBF can mediate progesterone-induced suppression of decidual lymphocyte cytotoxicity (Laskarin et al 2002). We predicted HLA-DPB1 as a target gene of hsa-miR-520f, which was low expressed in villus (Supplementary Table 3), and just as Takakuwa et al (2003) reported HLA-DPB1 might be a risk allele for unexplained recurrent abortion in the Japanese population.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients

Dong¹,

Zhang²,

Xia³

et al. 2014

Reproduction

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MicroRNAs (miRNAs) are non-coding RNA molecules of about 22 nucleotides that involved in post-transcriptional gene regulation. Evidence indicates that miRNAs play essential roles in endometriosis, pre-eclampsia, infertility and other reproductive system diseases. However, whether miRNAs are involved in recurrent spontaneous abortion (RSA) is unclear. In this work, we analysed the miRNA expression profiles in six pairs of villus or decidua from RSA patients and normal pregnancy (NP) women using a human miRNA microarray. Some of the chip results were confirmed by RT-qPCR. In the villi of RSA patients, expression of hsa-miR-184, hsa-miR-187 and hsa-miR-125b-2 was significantly higher, while expression of hsa-miR-520f, hsa-miR-3175 and hsa-miR-4672 was significantly lower, comparing with those of NP control. As well, a total of five miRNAs (hsa-miR-517c, hsa-miR-519a-1, hsa-miR-522, hsa-miR-520h and hsa-miR-184) were upregulated in the decidua of RSA patients. The target genes of these differentially expressed miRNAs were predicted by miRWalk, and we speculate a network of miRNA regulating RSA by target genes function on adhesion, apoptosis and angiogenesis. Our study may help clarify the molecular mechanisms which are involved in the progression of RSA, and provide a reference for future research.

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“…It has been shown that a mediator of progesterone called progesteroneinduced blocking factor blocked the dNK lytic activity [47].…”

Section: Dnk Cells Are Very Unlikely To Play a Role In Cytotoxicitymentioning

confidence: 99%

HLA Class I/NK Cell Receptor Interaction in Early Human Decidua basalis: Possible Functional Consequences

Rabot

Tabiasco

Polgar

et al. 2005

Chemical Immunology and Allergy

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Human decidual NK (dNK) cells differ from their peripheral blood (PB)-NK counterparts. The major subset of PB-NK is CD56dim, CD16+, CD160+ (highly cytolytic), whereas the major subpopulation of dNK is CD56bright, CD16- and CD160- (high cytokine producer). Extravillous cytotrophoblast invading the decidua basalis in early pregnancy expresses the polymorphic HLA-C, and nonpolymorphic HLA-E and HLA-G molecules that can interact with specific HLA class I-dependent dNK receptors, including the immunoglobulin-like KIRs, the lectin-like CD94/NKG2 and the CD160 receptors. There is no clear evidence thus far that dNK cells kill trophoblast cells. Instead they are able to secrete cytokines which are likely to be beneficial for the placental development, maternal uterine spiral arteries remodeling, and the antiviral immune response.

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Progesterone Induced Blocking Factor (PIBF) Mediates Progesterone Induced Suppression of Decidual Lymphocyte Cytotoxicity

Abstract: The results indicate possible role for PIBF, as a mediator of progesterone in regulation of DL cytolytic activity at the maternal-foetal (M-F) interface.

Cited by 57 publications

References 31 publications

Transcriptional Profiling of Human Endocervical Tissues Reveals Distinct Gene Expression in the Follicular and Luteal Phases of the Menstrual Cycle1

Transcriptional Profiling of Human Endocervical Tissues Reveals Distinct Gene Expression in the Follicular and Luteal Phases of the Menstrual Cycle1

Genome-wide miRNA profiling of villus and decidua of recurrent spontaneous abortion patients

HLA Class I/NK Cell Receptor Interaction in Early Human Decidua basalis: Possible Functional Consequences

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