Progesterone Increases Bifidobacterium Relative Abundance during Late Pregnancy

Nuriel-Ohayon, Meital; Neuman, Hadar; Ziv, Oren; Belogolovski, Anna; Barsheshet, Yiftah; Bloch, Naamah; Uzan, Atara; Lahav, Roey; Peretz, Avi; Frishman, S.; Hod, Moshe; Hadar, Eran; Louzoun, Yoram; Avni, Orly; Koren, Omry

doi:10.1016/j.celrep.2019.03.075

Cited by 154 publications

(137 citation statements)

References 36 publications

Supporting

Mentioning

128

Contrasting

Unclassified

Order By: Relevance

“…However, we are not the only group who found differences in gut microbiota during pregnancy. Also previous studies in mice have shown an effect of pregnancy on the abundance of gut microbiota species (14,36,37). However, each of these mouse studies found their own specific adaptations of the microbiota to pregnancy.…”

Section: Discussionmentioning

confidence: 97%

See 1 more Smart Citation

Microbiota Induced Changes in the Immune Response in Pregnant Mice

et al. 2020

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 97%

“…The mechanism changing the microbiota during pregnancy are unclear at this time. However, it has been suggested that pregnancy hormones, like progesterone, may affect the gut microbiota (36). Also, diet has been shown to affect the gut microbiota (37).…”

Section: Discussionmentioning

confidence: 99%

Microbiota Induced Changes in the Immune Response in Pregnant Mice

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Bacterial 16S rRNA gene sequences from each sample were amplified by PCR performed in 96-well plates. Sample preparation was similar to that described by Nuriel-Ohayon et al [ 25 ]. Briefly, PCR reactions contained 17 μL PCR grade nuclease-free water (Hylabs, Rehovot, Israel), 25 μL PrimeSTAR Max (Takara-Clontech, Shiga, Japan), 2 μL each of the forward and reverse primers (10 μM final concentration), and 4 μL genomic DNA.…”

Section: Methodsmentioning

confidence: 99%

“…Reactions were heated to 95 °C for 3 min for DNA denaturation, with amplification proceeding for 33 cycles at 98 °C for 10 min, 55 °C for 5 min, and 72 °C for 5 min; a final extension of 1 min at 72 °C was added to ensure complete amplification. Barcoded universal primers 515F and 806R containing Illumina adapter sequences which target the highly conserved V4 region, as previously described [ 25 ], were used to amplify the microbiota from individual samples. Amplicons were purified using AMPure XP magnetic beads (Beckman Coulter, Brea, CA) and subsequently quantified using Quant-it Picogreen dsDNA quantification kit (Invitrogen, Carlsbad, CA).…”

Section: Methodsmentioning

confidence: 99%

Microbial signature in IgE-mediated food allergies

et al. 2020

Self Cite

View full text Add to dashboard Cite

Background Multiple studies suggest a key role for gut microbiota in IgE-mediated food allergy (FA) development, but to date, none has studied it in the persistent state. Methods To characterize the gut microbiota composition and short-chain fatty acid (SCFAs) profiles associated with major food allergy groups, we recruited 233 patients with FA including milk (N = 66), sesame (N = 38), peanut (N = 71), and tree nuts (N = 58), and non-allergic controls (N = 58). DNA was isolated from fecal samples, and 16S rRNA gene sequences were analyzed. SCFAs in stool were analyzed from patients with a single allergy (N = 84) and controls (N = 31). Results The gut microbiota composition of allergic patients was significantly different compared to age-matched controls both in α-diversity and β-diversity. Distinct microbial signatures were noted for FA to different foods. Prevotella copri (P. copri) was the most overrepresented species in non-allergic controls. SCFAs levels were significantly higher in the non-allergic compared to the FA groups, whereas P. copri significantly correlated with all three SCFAs. We used these microbial differences to distinguish between FA patients and non-allergic healthy controls with an area under the curve of 0.90, and for the classification of FA patients according to their FA types using a supervised learning algorithm. Bacteroides and P. copri were identified as taxa potentially contributing to KEGG acetate-related pathways enriched in non-allergic compared to FA. In addition, overall pathway dissimilarities were found among different FAs. Conclusions Our results demonstrate a link between IgE-mediated FA and the composition and metabolic activity of the gut microbiota.

show abstract

“…Bifidobacterium is a genus that influences intestinal function in infants who have received healthy breast milk, while low but relatively stable Bifidobacterium counts are observed in adulthood [39]. The abundance of Bifidobacterium increases in the late stages of pregnancy in both women and mice, indicating the causative role of progesterone [40]. Bifidobacterium bifidum colonization increases interleukin 6 (IL-6) and IL-8 cytokine levels through NF-κB activation in mice [41].…”

Section: Discussionmentioning

confidence: 99%

Altered gut microbiota due to daily intake of flavonoid-rich orange juice regulate depressive symptoms

Park¹,

Choi²,

Lee³

2020

Preprint

View full text Add to dashboard Cite

Background: Depression is not just a general mental health problem but a serious medical illness that can worsen without treatment. The gut microbiome plays a major role in the two-way communication system between the intestines and brain. The current study examined the effects of flavonoids on depression by observing the changes in the gut microbiome and depressive symptoms of young participants consuming flavonoid-rich orange juice. Results: All participants (Depression) were evaluated using the Center for Epidemiological Studies Depression Scale (CES-D), a psychiatric screening tool used to detect preexisting psychiatric disorders, and were randomly divided into two groups: the orange juice (Depression_O) and flavored placebo (Depression_F) groups. For 8 weeks, flavonoid-rich orange juice (157.9±1.4 mg/100 g) or flavored placebo drink (28.4±0.7 mg/100 g) were consumed twice daily before breakfast and dinner. In total, 80 fecal samples from 40 participants (mean age, 21.83 years) were sequenced. Regarding depression, positive correlations were observed between brain-derived neurotrophic factor and the Lachnospiraceae family (Lachnospiraceae_uc and Murimonas) before flavonoid-rich orange juice treatment. Most notably, the abundance of the Lachnospiraceae family (Lachnospiraceae_uc, Eubacterium_g4, Roseburia_uc, Coprococcus_g2_uc, Agathobacter_uc) had increased after flavonoid-rich orange juice treatment compared to that after flavored placebo treatment. We also validated the presence of unclassified Lachnospiraceae through sensitive real-time quantitative polymerase chain reaction using stool samples from participants before and after flavonoid treatment. Conclusions: Our results provide novel interventional evidence that alteration in the microbiome due to flavonoid treatment is related to potential improvement in depression in young adults. Trial registration: This study was registered on the Clinical Research Information Service (CRIS) with the registration number KCT0004865.

show abstract

Progesterone Increases Bifidobacterium Relative Abundance during Late Pregnancy

Cited by 154 publications

References 36 publications

Microbiota Induced Changes in the Immune Response in Pregnant Mice

Microbiota Induced Changes in the Immune Response in Pregnant Mice

Microbial signature in IgE-mediated food allergies

Altered gut microbiota due to daily intake of flavonoid-rich orange juice regulate depressive symptoms

Contact Info

Product

Resources

About