Progesterone binding and inhibition of growth in Trichophyton mentagrophytes

Abstract

Specific binding of [3H]progesterone to cytosol of Trichophyton mentagrophytes was demonstrated. Scatchard analysis of [3H]progesterone binding showed a single class of binding sites with a dissociation constant of 9.5x 108 ± 2.4 x 10-8 M (standard deviation) and a maximal binding capacity of 4,979 ± 3,489 fmol/mg of cytosol protein. Deoxycorticosterone and dihydrotestosterone competitively inhibited binding by 50% at molar ratios of 10:1 and 20:1, respectively. Other steroid hormones that were tested had minimal activity, indicating binding specificity. Steroid hormone actions in T. mentagrophytes were examined in growth studies. Growth was assessed by determination of cellular ATP content. Progesterone inhibited growth in a dose-responsive manner, with a 50% inhibition concentration of 5.5 x 10-6 M. Partial recovery from inhibition occurrred after 24 to 48 h; inhibition could be enhanced by dividing the amount of added progesterone every 24 h. In the same rank order as was their relationship to each other and progesterone in binding studies, deoxycorticosterone and dihydrotestosterone were less effective inhibitors; other steroid hormones that were tested showed no consistent effect. We hypothesize that the binder described, acting as a hormone receptor, is the molecular site of action for the functional effect of the hormone. The functional effect may be related to the observed resistance of females to dermatophytosis. on July 16, 2020 by guest http://iai.asm.org/ Downloaded from on July 16, 2020 by guest http://iai.asm.org/ Downloaded from on July 16, 2020 by guest http://iai.asm.org/ Downloaded from

Human androgenic steroids affect growth of dermatophytes in vitro

“…Mean thallus diameters in % of control for progesterone [4,5]. However, the hormonal concentrations required to inhibit fungal growth are much higher.…”

Review: Microbial endocrinology: intersection of microbiology and neurobiology matters to swine health from infection to behavior

“…The range of neurochemicals and neurohormones isolated from microorganisms is extensive and additionally have been demonstrated to show biological activity in mammalian cells. These include serotonin (Sridharan et al, 2014); catecholamines, such as norepinephrine and dopamine (Asano et al, 2012) (Tsavkelova et al, 2000;Malikina et al, 2010); progesterone (Schar et al, 1986); somatostatin (LeRoith et al, 1985); and even the primary inhibitor neurotransmitter in the brain, γ amino butyric acid (GABA) (Barrett et al, 2012). For a more complete discussion of the ubiquitous presence of neurochemicals throughout the various biological kingdoms see the comprehensive review (Roshchina, 2016).…”

Effects of Insulin, Norepinephrine and Estradiol on Growth of Different Microorganisms

“…Memeliler gibi kimi mikroorganizmaların da hormon üretmesi, memeli hormonlarını tanıyan reseptörleri taşımaları ve hormonların memelimikrop ilişkisinde sinyal molekülü olarak rol oynadığının gösterilmesi ile "Mikrobiyal Endokrinoloji" olarak adlandırılan yeni bir araştırma alanı doğmuştur. Escherichia coli'de insülin, Tetrahymena pyriformis'te kortikortropin, Bacillus subtilis ve Plasmodium falciparum'da somatostatin, Trychophyton mentagrophytes'de progesteron; Saccharomyces cerevisiae, Paracoccidioides brasiliensis, Candida albicans ve Pseudomonas aeruginosa'da östradiol ile ilgili reseptörlerin varlığı gösterilmiştir (11)(12)(13)(14)(15)(16)(17)(18)(19) .…”