Progesterone and 20α-Hydroxypregn-4-en-3-one in Plasma, Ovaries and Uteri During Pregnancy in the Rat<sup>1</sup>

Wiest, Walter G.

doi:10.1210/endo-87-1-43

Cited by 168 publications

(71 citation statements)

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“…It was also suggested that P-gp may be involved in the transport of P4 across the uterine epithelium during pregnancy (Arceci et al 1990). The gestational profile of Abcb1a/1b mRNA described in this study (increase at late gestation and dramatic decrease during labor) is mirrored by the changes in plasma P4 levels in the rat (Wiest 1970), indicating that it might be directly regulated by this ovarian hormone. We further show that a blockade of P4 signaling by RU486 causes a rapid decrease, while the maintenance of high P4 levels prevents the drop in Abcb1a/1b expression during term labor.…”

Section: Discussionsupporting

confidence: 50%

P-glycoprotein expression and localization in the rat uterus throughout gestation and labor

et al. 2016

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Uterine tissues contain the efflux transporter P-glycoprotein (P-gp, encoded by Abcb1a/1b gene), but little is known about how it changes through gestation. Our aim was to investigate the expression profile and cellular localization of P-gp in the pregnant, laboring and post-partum (PP) rat uterus. We propose that during pregnancy the mechanical and hormonal stimuli play a role in regulating myometrial Abcb1a/1b/P-gp. Samples from bilaterally and unilaterally pregnant rats were collected throughout gestation, during labor, and PP (n = 4-6/gestational day). RNA and protein were isolated and subjected to quantitative PCR and immunoblotting; P-gp transcript and protein were localized by in situ hybridization and immunohistochemistry. Expression of Abcb1a/1b gene and membrane P-gp protein in uterine tissue (1) increased throughout gestation, peaked at term (GD19-21) and dropped during labor (GD23L); and (2) was upregulated only in gravid but not in empty horn of unilaterally pregnant rats. (3) The drop of Abcb1a/1b mRNA on GD23 was prevented by artificial maintenance of elevated progesterone (P4) levels in late gestation; (4) injection of the P4 receptor antagonist RU486 on GD19 caused a significant decrease in Abcb1 mRNA levels. (5) In situ hybridization and immunohistochemistry indicated that Abcb1/P-gp is absent from myometrium throughout gestation; (6) was expressed exclusively by uterine microvascular endothelium (at early gestation) and luminal epithelium (at mid and late gestation), but was undetectable during labor. In conclusion, ABC transporter protein P-gp in pregnant uterus is hormonally and mechanically regulated. However, its substrate(s) and precise function in these tissues during pregnancy remains to be determined. Reproduction (2016) 152 195-204

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Section: Discussionsupporting

confidence: 50%

P-glycoprotein expression and localization in the rat uterus throughout gestation and labor

et al. 2016

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“…(Short, 1961;Stabenfeldt, Osburn & Ewing, 1970) and goat (Thorburn & Schneider, 1971), while in others, there is a considerable increase in progesterone after implantation, e.g. in the ewe (Bassett, Oxborrow, Smith & Thorburn, 1969), coypu (Rowlands & Heap, 1966) and rat (Wiest, 1970). A similar profile of progesterone secretion during pregnancy is observed in other canids, such as the blue fox (Moller, 1973) and the domestic dog (Parkes, Bell & Christie, 1972;Smith & McDonald, 1974;Hadley, 1975;Graf, 1978).…”

Section: Methodsmentioning

confidence: 71%

Oestrogen and progesterone concentrations in peripheral blood in pregnant red foxes (Vulpes vulpes)

Bonnin¹,

Mondain-Monval²,

Dutourné³

1978

Reproduction

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Summary. Oestrogen levels were low during most of gestation, but there was a significant increase (P <0\m=.\05) in oestradiol concentrations at implantation. Early pregnancy was characterized by high levels of progesterone which decreased significantly (P < 0\m=.\001)thereafter, but there was no decline in progesterone or rise in oestrogen levels at parturition. There was no difference in the length of progesterone secretion between pregnant and non-pregnant females.

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“…McCormick & Senior (1971) have also shown that oestrogens in optimal doses raise the concentration of plasma kininogen in intact and ovariectomized non-pregnant female rats. It has been shown by Yoshinaga, Hawkins & Stocker (1969) and Wiest (1970) that oestrogens and progesterone, respectively, undergo very marked changes in the blood towards the end of gestation in the rat. This paper describes experiments to study the effect of oestrogens and progesterone alone and in combination on plasma kininogen levels in the non-pregnant female rat.…”

Section: Introductionmentioning

confidence: 99%

Variation in the Oestrogen-Progesterone Ratio and Its Effect on Plasma Kininogen Levels in the Rat

Senior¹,

Whalley²

1974

Reproduction

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Summary. An increase in plasma kininogen levels in the rat has been demonstrated in response to treatment with oestrogens. The effect is dose-dependent and occurs within 5 days of the onset of treatment. Progesterone has little effect on the plasma kininogen levels in doses below 10 mg/kg. In these high doses, progesterone produces a fall in the plasma kininogen levels within 3 days. If oestrogen and progesterone are administered simultaneously for 5 days, the plasma kininogen concentration varies according to the oestrogen-progesterone ratio. It is suggested that changes in the oestrogen-progesterone ratio in the rat during pregnancy may be responsible for the changes observed in the plasma kininogen concentration.

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Progesterone and 20α-Hydroxypregn-4-en-3-one in Plasma, Ovaries and Uteri During Pregnancy in the Rat¹

Cited by 168 publications

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P-glycoprotein expression and localization in the rat uterus throughout gestation and labor

P-glycoprotein expression and localization in the rat uterus throughout gestation and labor

Oestrogen and progesterone concentrations in peripheral blood in pregnant red foxes (Vulpes vulpes)

Variation in the Oestrogen-Progesterone Ratio and Its Effect on Plasma Kininogen Levels in the Rat

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Progesterone and 20α-Hydroxypregn-4-en-3-one in Plasma, Ovaries and Uteri During Pregnancy in the Rat1

Cited by 168 publications

References 0 publications

P-glycoprotein expression and localization in the rat uterus throughout gestation and labor

P-glycoprotein expression and localization in the rat uterus throughout gestation and labor

Oestrogen and progesterone concentrations in peripheral blood in pregnant red foxes (Vulpes vulpes)

Variation in the Oestrogen-Progesterone Ratio and Its Effect on Plasma Kininogen Levels in the Rat

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Progesterone and 20α-Hydroxypregn-4-en-3-one in Plasma, Ovaries and Uteri During Pregnancy in the Rat¹