Progenitor cells trapped in marrow filters can reduce GvHD and transplant mortality

Vicente, Danielly C.; Podestà, Marina; Pitto, A.; Pozzi, Sarah; Lucchetti, S.; Lamparelli, Teresa; Tedone, Elisabetta; Ibatici, Adalberto; Figari, O; Frassoni, Francesco; Lint, Maria Teresa Van; Piaggio, Giovanna; Sacchi, Nicoletta; Bacigalupo, Andrea

doi:10.1038/sj.bmt.1705413

Cited by 10 publications

(7 citation statements)

References 20 publications

(18 reference statements)

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“…It is been reported, for example, that filters used in bone marrow harvest may contain on average 21% long-term culture-initiating cells and 15% fibroblast colony-forming units of the total progenitor cell content and that adding these cells to the harvest significantly reduced the incidence of grade II-IV GvHD and the transplant-related mortality. 15 It highlights the importance of other bone marrow cells, such as mesenchymal cells in the mechanisms of engraftment. The evaluation of chimerism at such low levels could help us understand the complex mechanism of genetically distinct cells interacting in different posttransplantation settings.…”

mentioning

confidence: 99%

Chimerism interpretation with a highly sensitive quantitative PCR method: 6 months median latency before chimerism drop below 0.1%

Vicente

Laranjeira

Miranda

et al. 2016

Bone Marrow Transplant

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confidence: 99%

Chimerism interpretation with a highly sensitive quantitative PCR method: 6 months median latency before chimerism drop below 0.1%

Vicente

Laranjeira

Miranda

et al. 2016

Bone Marrow Transplant

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“…As controls in flow cytometer study, BM aspirates were obtained from healthy BM donors, selected according to the Transplant Unit Clinical Protocol of Ematologia 2 at the IRCCS San Martino-IST in Genoa, following a written informed consent at the time of donation. Samples were processed as described [ 40 ]. At the end of processing, an aliquot was taken to perform quality control tests.…”

Section: Methodsmentioning

confidence: 99%

Altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma

et al. 2017

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Neuroblastoma (NB) is a pediatric tumor presenting at diagnosis either as localized or metastatic disease, which mainly involves the bone marrow (BM). The physical occupancy of BM space by metastatic NB cells has been held responsible for impairment of BM function. Here, we investigated whether localized or metastatic NB may alter hematopoietic lineages’ maturation and release of mature cells in the periphery, through gene expression profiling, analysis of BM smears, cell blood count and flow cytometry analysis.Gene ontology and disease-associated analysis of the genes significantly under-expressed in BM resident cells from children with localized and metastatic NB, as compared to healthy children, indicated anemia, blood group antigens, and heme and porphyrin biosynthesis as major functional annotation clusters. Accordingly, in children with NB there was a selective impairment of erythrocyte maturation at the ortho-chromic stage that resulted in reduced erythrocyte count in the periphery, regardless of the presence of metastatic cells in the BM. By considering all NB patients, low erythrocyte count at diagnosis associated with worse survival. Moreover, in the subset of metastatic patients, low erythrocyte count, hemoglobin and hematocrit and high red cell distribution width at follow-up also associated with worse outcome.These observations provide an alternative model to the tenet that infiltrating cells inhibit BM functions due to physical occupancy of space and may open a new area of research in NB to understand the mechanism(s) responsible for such selective impairment.

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“…Samples were processed as described in [13], and an aliquot was taken at the end of processing to perform quality control tests, such as CD34 + cell count, in vitro progenitors' cell growth, and sterility. The remaining BM blood sample from this aliquot was subjected to centrifugation (3000 g × 10′) to obtain BM plasma.…”

Section: Methodsmentioning

confidence: 99%

Soluble HLA-G and HLA-E Levels in Bone Marrow Plasma Samples Are Related to Disease Stage in Neuroblastoma Patients

Morandi

Pozzi

Carlini

et al. 2016

Journal of Immunology Research

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The role of nonclassical HLA-class Ib molecules HLA-G and HLA-E in the progression of Neuroblastoma (NB), the most common pediatric extracranial solid tumor, has been characterized in the last years. Since BM infiltration by NB cells is an adverse prognostic factor, we have here analyzed for the first time the concentration of soluble (s)HLA-G and HLA-E in bone marrow (BM) plasma samples from NB patients at diagnosis and healthy donors. sHLA-G and sHLA-E are present in BM plasma samples, and their levels were similar between NB patients and controls, thus suggesting that these molecules are physiologically released by resident or stromal BM cell populations. This hypothesis was supported by the finding that sHLA-G and sHLA-E levels did not correlate with BM infiltration and other adverse prognostic factors (MYCN amplification and age at diagnosis). In contrast, BM plasma levels of both molecules were higher in patients with metastatic disease than in patients with localized NB, thus suggesting that concentration of these molecules might be correlated with disease progression. The prognostic role of sHLA-G and sHLA-E concentration in the BM plasma for NB patients will be evaluated in future studies, by analyzing the clinical outcome of the same NB patients at follow-up.

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Progenitor cells trapped in marrow filters can reduce GvHD and transplant mortality

Cited by 10 publications

References 20 publications

Chimerism interpretation with a highly sensitive quantitative PCR method: 6 months median latency before chimerism drop below 0.1%

Chimerism interpretation with a highly sensitive quantitative PCR method: 6 months median latency before chimerism drop below 0.1%

Altered erythropoiesis and decreased number of erythrocytes in children with neuroblastoma

Soluble HLA-G and HLA-E Levels in Bone Marrow Plasma Samples Are Related to Disease Stage in Neuroblastoma Patients

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