Progenitor Cells from the Porcine Neural Retina Express Photoreceptor Markers After Transplantation to the Subretinal Space of Allorecipients

Klassen, Henry; Kiilgaard, Jens Folke; Zahir, Tasneem; Ziaeian, Boback; Kirov, Ivan I.; Scherfig, Erik; Warfvinge, Karin; Young, Michael J.

doi:10.1634/stemcells.2006-0541

Cited by 86 publications

(73 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Rods were specifically detected by using an antibody against rhodopsin, i.e., Rho4D2, and the staining result was in parallel with that of the original description of the staining pattern (Hicks and Molday 1986) and with experimental in vitro and in vivo porcine studies performed by us and others (Gaudin et al 1996;Warfvinge et al 2005;Ghosh et al 2007;Klassen et al 2007Klassen et al ,2008. The transducin-staining pattern was in agreement with those of recent studies where the marker was used as a parameter in phenotypic differentiation in retinal development, as well as transplantation studies (Mahmoud et al 2003;Engelsberg et al 2005;Engelsberg and Ghosh 2007;Ghosh et al 2007;Klassen et al 2007Klassen et al ,2008. Photoreceptor outer segments were identified using the novel marker ROM-1.…”

Section: Fixation Methods and Tissue Preparationsupporting

confidence: 73%

“…Then, the posterior segment was fixed by immersion for 2 hr. This method results in a thorough and even fixation and was chosen based on our recent successful histological results (Warfvinge et al 2005(Warfvinge et al ,2006Klassen et al 2007Klassen et al ,2008. The specimen preparation method also plays a role in the histochemical properties of the retinal tissue.…”

Section: Fixation Methods and Tissue Preparationmentioning

confidence: 99%

“…Here, recoverin visualized rods and cones. In the pig, recoverin has been widely used as a marker in studies of retinogenesis and photoreceptor degeneration (Petters et al 1997;Li et al 1998;Chandler et al 1999;Traverso et al 2003;Engelsberg et al 2005;Engelsberg and Ghosh 2007;Gu et al 2007;Klassen et al 2007Klassen et al ,2008. Rods were specifically detected by using an antibody against rhodopsin, i.e., Rho4D2, and the staining result was in parallel with that of the original description of the staining pattern (Hicks and Molday 1986) and with experimental in vitro and in vivo porcine studies performed by us and others (Gaudin et al 1996;Warfvinge et al 2005;Ghosh et al 2007;Klassen et al 2007Klassen et al ,2008.…”

Section: Fixation Methods and Tissue Preparationmentioning

confidence: 99%

“…In the pig, recoverin has been widely used as a marker in studies of retinogenesis and photoreceptor degeneration (Petters et al 1997;Li et al 1998;Chandler et al 1999;Traverso et al 2003;Engelsberg et al 2005;Engelsberg and Ghosh 2007;Gu et al 2007;Klassen et al 2007Klassen et al ,2008. Rods were specifically detected by using an antibody against rhodopsin, i.e., Rho4D2, and the staining result was in parallel with that of the original description of the staining pattern (Hicks and Molday 1986) and with experimental in vitro and in vivo porcine studies performed by us and others (Gaudin et al 1996;Warfvinge et al 2005;Ghosh et al 2007;Klassen et al 2007Klassen et al ,2008. The transducin-staining pattern was in agreement with those of recent studies where the marker was used as a parameter in phenotypic differentiation in retinal development, as well as transplantation studies (Mahmoud et al 2003;Engelsberg et al 2005;Engelsberg and Ghosh 2007;Ghosh et al 2007;Klassen et al 2007Klassen et al ,2008.…”

Section: Fixation Methods and Tissue Preparationmentioning

confidence: 99%

“…The overall staining pattern of GFAP is similar to that reported in recent studies using the adult pig (Kiilgaard et al 2002;Warfvinge et al 2005;Iandiev et al 2006;Ghosh et al 2007). Glial differentiation determined by vimentin and GFAP expression levels was studied in in vitro studies of porcine retinal progenitors and after grafting of these tissues in vivo (García et al 2002;Gu et al 2007;Klassen et al 2007Klassen et al ,2008.…”

Section: Specificity Of Glial Antibodies Bfgf Gfap and Vimentinmentioning

confidence: 99%

See 4 more Smart Citations

A Battery of Cell- and Structure-specific Markers for the Adult Porcine Retina

Johansson

Eftekhari

Warfvinge

2010

J Histochem Cytochem.

Self Cite

View full text Add to dashboard Cite

S U M M A R Y The pig is becoming an increasingly used non-primate model in experimental studies of human retinal diseases and disorders. The anatomy, size, and vasculature of the porcine eye and retina closely resemble their human counterparts, which allows for application of standard instrumentation and diagnostics used in the clinic. Despite many reports that demonstrate immunohistochemistry as a useful method for exploring neuropathological changes in the mammalian central nervous system, including the pig, the porcine retina has been sparsely described. Hence, to facilitate further immunohistochemical analysis of the porcine retina, we report on the successful use of a battery of antibodies for staining of paraformaldehyde-fixed cryosectioned retina. The following antibodies were evaluated for neuronal cells and structures: recoverin (cones and rods), Rho4D2 (rods), transducin-g (cones), ROM-1 (photoreceptor outer segments), calbindin (horizontal cells), PKC-a (bipolar cells), parvalbumin (amacrine and displaced amacrine cells), and NeuN (ganglion cells and displaced amacrines). For detecting synaptic connections in fiber layers, we used an antibody against synaptobrevin. For detecting retinal pigment epithelium, we studied antibodies against cytokeratin and RPE65, respectively. The glial cell markers used were bFGF (Müller cells and displaced amacrine cells), GFAP (Müller cells and astrocytes), and vimentin (Müller cells). Each staining effect was evaluated with regard to its specificity, sensitivity, and reproducibility in the identification of individual cells, specific cell structures, and fiber layers, respectively. The markers parvalbumin and ROM-1 were tested here for the first time for the porcine retina. All antibodies tested resulted in specific staining of high quality. In conclusion, all immunohistochemical protocols presented here will be applicable in fixed, cryosectioned pig retina. (J Histochem Cytochem 58:377-389, 2010)

show abstract

Section: Fixation Methods and Tissue Preparationsupporting

confidence: 73%

Section: Fixation Methods and Tissue Preparationmentioning

confidence: 99%

Section: Fixation Methods and Tissue Preparationmentioning

confidence: 99%

Section: Fixation Methods and Tissue Preparationmentioning

confidence: 99%

Section: Specificity Of Glial Antibodies Bfgf Gfap and Vimentinmentioning

confidence: 99%

See 3 more Smart Citations

A Battery of Cell- and Structure-specific Markers for the Adult Porcine Retina

Johansson

Eftekhari

Warfvinge

2010

J Histochem Cytochem.

Self Cite

View full text Add to dashboard Cite

show abstract

Retinal Stem Cells and Regeneration of Vision System

Yip

2013

The Anatomical Record

View full text Add to dashboard Cite

The vertebrate retina is a well-characterized model for studying neurogenesis. Retinal neurons and glia are generated in a conserved order from a pool of mutlipotent progenitor cells. During retinal development, retinal stem/progenitor cells (RPC) change their competency over time under the influence of intrinsic (such as transcriptional factors) and extrinsic factors (such as growth factors). In this review, we summarize the roles of these factors, together with the understanding of the signaling pathways that regulate eye development. The information about the interactions between intrinsic and extrinsic factors for retinal cell fate specification is useful to regenerate specific retinal neurons from RPCs. Recent studies have identified RPCs in the retina, which may have important implications in health and disease. Despite the recent advances in stem cell biology, our understanding of many aspects of RPCs in the eye remains limited. PRCs are present in the developing eye of all vertebrates and remain active in lower vertebrates throughout life. In mammals, however, PRCs are quiescent and exhibit very little activity and thus have low capacity for retinal regeneration. A number of different cellular sources of RPCs have been identified in the vertebrate retina. These include PRCs at the retinal margin, pigmented cells in the ciliary body, iris, and retinal pigment epithelium, and M€ uller cells within the retina. Because PRCs can be isolated and expanded from immature and mature eyes, it is possible now to study these cells in culture and after transplantation in the degenerated retinal tissue. We also examine current knowledge of intrinsic RPCs, and human embryonic stems and induced pluripotent stem cells as potential sources for cell transplant therapy to regenerate the diseased retina. Anat Rec, 297:137-160,

show abstract

Animal modelling for inherited central vision loss

Kostic

Arsenijévic

2015

The Journal of Pathology

View full text Add to dashboard Cite

Disease‐causing variants of a large number of genes trigger inherited retinal degeneration leading to photoreceptor loss. Because cones are essential for daylight and central vision such as reading, mobility, and face recognition, this review focuses on a variety of animal models for cone diseases. The pertinence of using these models to reveal genotype/phenotype correlations and to evaluate new therapeutic strategies is discussed. Interestingly, several large animal models recapitulate human diseases and can serve as a strong base from which to study the biology of disease and to assess the scale‐up of new therapies. Examples of innovative approaches will be presented such as lentiviral‐based transgenesis in pigs and adeno‐associated virus (AAV)‐gene transfer into the monkey eye to investigate the neural circuitry plasticity of the visual system. The models reported herein permit the exploration of common mechanisms that exist between different species and the identification and highlighting of pathways that may be specific to primates, including humans. © 2015 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.

show abstract

Progenitor Cells from the Porcine Neural Retina Express Photoreceptor Markers After Transplantation to the Subretinal Space of Allorecipients

Abstract: ABSTRACT

Cited by 86 publications

References 33 publications

A Battery of Cell- and Structure-specific Markers for the Adult Porcine Retina

A Battery of Cell- and Structure-specific Markers for the Adult Porcine Retina

Retinal Stem Cells and Regeneration of Vision System

Animal modelling for inherited central vision loss

Contact Info

Product

Resources

About