Progenitor Cells From Dental Follicle Are Able to Form Cementum Matrix In Vivo

Handa, Keisuke; Saito, Masahiro; Tsunoda, Akira; Yamauchi, Masato; Hattori, Shintaro; Sato, Sadao; Toyoda, Minoru; Teranaka, Toshio; Narayanan, A. Sampath

doi:10.1080/03008200290001023

Cited by 125 publications

(84 citation statements)

References 4 publications

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“…These cells can be maintained in culture for at least 15 passages. STRO-1 positive DFSC can differentiate into cementoblasts in vitro (Kemoun et al, 2007) and are able to form cementum in vivo (Handa et al, 2002). Immortalized dental follicle cells are able to re-create a new periodontal ligament (PDL) after in vivo implantation (Yokoi et al, 2007).…”

Section: Stem Cells In Regenerative Medicinementioning

confidence: 99%

Stem cells for tooth engineering

Bluteau

Luder

Bari³

et al. 2008

eCM

168

152

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Tooth development results from sequential and reciprocal interactions between the oral epithelium and the underlying neural crest-derived mesenchyme. The generation of dental structures and/or entire teeth in the laboratory depends upon the manipulation of stem cells and requires a synergy of all cellular and molecular events that finally lead to the formation of tooth-specific hard tissues, dentin and enamel. Although mesenchymal stem cells from different origins have been extensively studied in their capacity to form dentin in vitro, information is not yet available concerning the use of epithelial stem cells. The odontogenic potential resides in the oral epithelium and thus epithelial stem cells are necessary for both the initiation of tooth formation and enamel matrix production. This review focuses on the different sources of stem cells that have been used for making teeth in vitro and their relative efficiency. Embryonic, post-natal or even adult stem cells were assessed and proved to possess an enormous regenerative potential, but their application in dental practice is still problematic and limited due to various parameters that are not yet under control such as the high risk of rejection, cell behaviour, long tooth eruption period, appropriate crown morphology and suitable colour. Nevertheless, the development of biological approaches for dental reconstruction using stem cells is promising and remains one of the greatest challenges in the dental field for the years to come.

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Section: Stem Cells In Regenerative Medicinementioning

confidence: 99%

Stem cells for tooth engineering

Bluteau

Luder

Bari³

et al. 2008

eCM

168

152

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“…For example, a 4-bp mutation in DLX3 is related with the TDO syndrome that is characterized with increased bone volume and mineral density but a decreased thickness of dentin [11,12,27,28]. DFCs reside in the dental follicle and are precursor cells for cementoblasts, periodontal ligament fibroblasts, and alveolar osteoblasts [1,2,29]. In this work we characterized DLX3 as an important transcription factor for the differentiation of DFCs into mineralized tissue cells such as alveolar bone osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

The Transcription Factor DLX3 Regulates the Osteogenic Differentiation of Human Dental Follicle Precursor Cells

Viale-Bouroncle

Felthaus²,

Schmalz³

et al. 2012

Stem Cells and Development

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The transcription factor DLX3 plays a decisive role in bone development of vertebrates. In neural-crest derived stem cells from the dental follicle (DFCs), DLX3 is differentially expressed during osteogenic differentiation, while other osteogenic transcription factors such as DLX5 or RUNX2 are not highly induced. DLX3 has therefore a decisive role in the differentiation of DFCs, but its actual biological effects and regulation are unknown. This study investigated the DLX3-regulated processes in DFCs. After DLX3 overexpression, DFCs acquired a spindlelike cell shape with reorganized actin filaments. Here, marker genes for cell morphology, proliferation, apoptosis, and osteogenic differentiation were significantly regulated as shown in a microarray analysis. Further experiments showed that DFCs viability is directly influenced by the expression of DLX3, for example, the amount of apoptotic cells was increased after DLX3 silencing. This transcription factor stimulates the osteogenic differentiation of DFCs and regulates the BMP/SMAD1-pathway. Interestingly, BMP2 did highly induce DLX3 and reverse the inhibitory effect of DLX3 silencing in osteogenic differentiation. However, after DLX3 overexpression in DFCs, a BMP2 supplementation did not improve the expression of DLX3 and the osteogenic differentiation. In conclusion, DLX3 influences cell viability and regulates osteogenic differentiation of DFCs via a BMP2-dependent pathway and a feedback control.

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“…Evidence indicates that cementum proteins in combination with BMP 2 are possible soluble factors involved in the chemotaxis and differentiation to cementoblastic lineage (Fig. 1) [33,34,66,[79][80][81][82][83].…”

Section: Stem Cell Niche and Stem Cell Differentiationmentioning

confidence: 99%

Periodontal Stem Cells: a Historical Background and Current Perspectives

Pitaru¹,

Narayanan

Etikala

et al. 2013

Curr Oral Health Rep

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In this review we discuss the historical perspective of stem cell populations from oral tissues in light of our current understanding of stem cell biology. Stem cells and their niches have been identified in the periodontium starting from the late 1970s. Applying new criteria for the identification and characterization reveals that oral tissues comprise a multipotent primitive neural crest-like stem cell population capable of differentiating into neural crest derived cell lineages of the cranial-facial zone. This population supplies cells to a more restricted stem cell type with tissue specific epigenetic memory that differentiates into cell lineages characteristic of their tissue origin. We believe that the microenvironment plays an essential role in maintaining stem cell populations and directing their migration and differentiation, and that this factor needs to be considered for utilization of stem cell-based therapy for periodontal regeneration and regenerative dental medicine.

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Progenitor Cells From Dental Follicle Are Able to Form Cementum Matrix In Vivo

Cited by 125 publications

References 4 publications

Stem cells for tooth engineering

Stem cells for tooth engineering

The Transcription Factor DLX3 Regulates the Osteogenic Differentiation of Human Dental Follicle Precursor Cells

Periodontal Stem Cells: a Historical Background and Current Perspectives

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