Mandates for biofuel and renewable electricity are creating incentives for biomass production in agricultural landscapes of the Upper Midwest. Different bioenergy crops are expected to vary in their effects on biodiversity and ecosystem services. Here, we use data from the North American Breeding Bird Survey to forecast the impact of potential bioenergy crops on avian species richness and the number of bird species of conservation concern in Midwestern landscapes. Our analysis suggests that expanded production of annual bioenergy crops (e.g., corn and soybeans) on marginal land will lead to declines in avian richness between 7% and 65% across 20% of the region, and will make managing at-risk species more challenging. In contrast, replacement of annual with diverse perennial bioenergy crops (e.g., mixed grasses and forbs) is expected to bring increases in avian richness between 12% and 207% across 20% of the region, and possibly aid the recovery of several species of conservation concern.agriculture | biofuel | diversity A variety of bioenergy crops are being considered for fuel, heat, and electricity production in the Upper Midwest. Candidate crops vary along gradients of plant diversity and perenniality. At one end of the spectrum are crops such as corn and soybeans, which are planted annually, enhanced with fertilizers and pesticides, and managed for minimum plant diversity [highinput low-diversity (HILD) crops] (1). At the other end of the spectrum are stands of native perennial grasses and forbs that, once established, are not replanted or treated with fertilizers and pesticides, and have relatively high plant diversity [low-input highdiversity (LIHD) crops] (1). The large volume of biomass needed to meet ethanol and renewable electricity mandates (2, 3) virtually guarantees that bioenergy crop choices will have far-reaching impacts, and requires that crops are chosen wisely.A number of studies have been conducted to compare the potential performance of candidate bioenergy crops. For example, quantitative analyses have been conducted to gauge the economic and energetic viability (4, 5) of these crops and their impacts on ecosystem processes such as carbon sequestration and nutrient loss (6, 7). However, there are few studies that consider how bioenergy crops will affect biodiversity (8, 9), and none that explicitly model biodiversity impacts under different bioenergy cropping scenarios. The lack of quantitative information regarding the biodiversity impacts of Midwestern bioenergy crops is cause for concern, given the extensive land-cover change that has occurred and the tenuous state of biodiversity in the region (10).How might different bioenergy crops influence biodiversity? Previous work suggests that animal diversity is driven, in part, by plant diversity and concomitant variation in plant chemistry, structure, and phenology (11)(12)(13)(14). Thus, we might hypothesize that landscapes dominated by HILD crops will host fewer animal species than those dominated by LIHD crops (8, 9). Indeed, several studie...