Profiling the rainbow trout hepatic miRNAome under diet-induced hyperglycemia

Abstract

Carnivorous rainbow trout exhibit prolonged postprandial hyperglycemia when fed a diet exceeding 20% carbohydrate content. This poor capacity to utilize carbohydrates has led to rainbow trout being classified as “glucose-intolerant” (GI). The metabolic phenotype has spurred research to identify the underlying cellular and molecular mechanisms of glucose intolerance, largely because carbohydrate-rich diets provide economic and ecological advantages over traditionally used fish meal, considered unsustainable for rainbow trout aquaculture operations. Evidence points to a contribution of hepatic intermediary carbohydrate and lipid metabolism, as well as upstream insulin signaling. Recently, microRNAs (miRNAs), small noncoding RNAs acting as negative posttranscriptional regulators affecting target mRNA stability and translation, have emerged as critical regulators of hepatic control of glucose-homeostasis in mammals, revealing that dysregulated hepatic miRNAs might play a role in organismal hyperglycemia in metabolic disease. To determine whether hepatic regulatory miRNA networks may contribute to GI in rainbow trout, we induced prolonged postprandial hyperglycemia in rainbow trout by using a carbohydrate-rich diet and profiled genome-wide hepatic miRNAs in hyperglycemic rainbow trout compared with fasted trout and trout fed a diet devoid of carbohydrates. Using small RNA next-generation sequencing and real-time RT-PCR validation, we identified differentially regulated hepatic miRNAs between these groups and used an in silico approach to predict bona fide mRNA targets and enriched pathways. Diet-induced hyperglycemia resulted in differential regulation of hepatic miRNAs compared with fasted fish. Some of the identified miRNAs, such as miRNA-27b-3p and miRNA-200a-3p, are known to be responsive to hyperglycemia in the liver of hyperglycemic glucose-tolerant fish and mammals, suggesting an evolutionary conserved regulation. Using Gene Ontology term-based enrichment analysis, we identify intermediate carbohydrate and lipid metabolism and insulin signaling as potential targets of posttranscriptional regulation by hyperglycemia-regulated miRNAs and provide correlative expression analysis of specific predicted miRNA-target pairs. This study identifies hepatic miRNAs in rainbow trout that exhibit differential postprandial expression in response to diets with different carbohydrate content and predicts posttranscriptionally regulated target mRNAs enriched for pathways involved in glucoregulation. Together, these results provide a framework for testable hypotheses of functional involvement of specific hepatic miRNAs in GI in rainbow trout.

Bacillus amyloliquefaciensameliorates high-carbohydrate diet-induced metabolic phenotypes by restoration of intestinal acetate-producing bacteria in Nile Tilapia

“…However, teleost fish are generally considered to be glucose intolerant (4) . An excess proportion of carbohydrates in their diet causes metabolic disturbance, including decreased growth performance (5) , persistent hyperglycaemia (6) and excess lipid deposition (7,8) . The glucose regulation mechanism in fish has been discussed in numerous studies; however, until now, most metabolic genes related to carbohydrate/glucose utilisation have been found to be conserved in vertebrates (9) .…”

A strain affiliated to Bacillus amyloliquefaciens alleviates high-carbohydrate diet- induced metabolic syndrome by restoration of acetate-producing bacteria in fish intestines

“…One of the metabolic disorders caused by HCD is the persistent hyperglycemia in fish [6,21]. To address whether the addition of B. amy SS1 to the HCD had a metabolic protective effect, the fasting glucose levels were detected.…”

“…An excess proportion of carbohydrates in their diet causes metabolic syndrome, including decreased growth performance [5], persistent hyperglycemia [6], and excess lipid deposition [7,8]. The glucose regulation mechanism in fish has been discussed in numerous studies; however, until now, most metabolic genes related to carbohydrate/glucose utilization have been found to be conserved in vertebrates [9].…”

Combined analysis of mRNA–miRNA reveals the regulatory roles of miRNAs in the metabolism of clam Cyclina sinensis hepatopancreas during acute ammonia nitrogen stress

“…Transcriptome sequencing (RNA-Seq) is one of the most effective method to provide insight on gene expression and relevant molecular pathways of species under specific condition with high resolution and sensitivity (Kostyniuk et al, 2019;Li et al, 2017). In recent years, using RNA-Seq to study the transcriptome response of aquatic animals under ammonia stress found that ammonia can significantly affect the gene expression profile (Gupta et al, 2014;Wolf, 2013).…”

“…It is reported to be sensitive to environmental changes (including ammonia, temperature, dissoluble oxygen and so on), which lead to their differential expression (Bizuayehu et al, 2015;Kostyniuk et al, 2019). Numerous studies have shown that differential expression of miRNA is one of the most key factors in regulating expression of target gene in aquatic animals to adapt to the environment (Sun et al, 2020).…”