Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array

Chen, Tao; Li, Zhenyan; Yu, Li; Li, Haicheng; Lin, Jin-Fei; Guo, Hongqian; Wang, Wei; Liang, Chen; Zhang, Xian-En; Wang, Yunxia; Chen, Yuhui; Liao, Qinghua; Tan, Yi; Shu, Yang; Huang, Wenyan; Cai, Changhui; Zhou, Zhongjing; Yu, Man; Li, Guozhou; Zhou, Lin; Zhong, Qian; Bi, Lijun; Zhao, Mengmeng; Guo, Liang; Zhou, Jie

doi:10.1016/j.tube.2015.12.007

Cited by 29 publications

(24 citation statements)

References 39 publications

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“…In fact, these mycobacterial‐specific cytokines have been proposed for use in immunodiagnostics . Interestingly, our data lend support to the theory that TB patients have a Th1/Th2 imbalance that is characterized by high IL‐4 but low IFN‐γ levels, a phenomenon that is a significant factor in the pathogenesis and development of TB . It is possible that this Th1/Th2 balance shift plays a role in the high cytotoxic background of CD8 + T cells.…”

Section: Discussionmentioning

confidence: 99%

Tuberculosis patients display a high proportion of CD8⁺ T cells with a high cytotoxic potential

Chávez‐Galán

Illescas‐Eugenio

Álvarez-Sekely

et al. 2019

Microbiology and Immunology

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Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (Mtb) and remains a major cause of morbidity and mortality worldwide. In the host's immune response system, T cells play a critical role in mediating protection against Mtb infection, but the role of CD8+ T cells is still controversial. We evaluated the phenotypical characterization and cytotoxic ability of CD8+ T cells by flow cytometry‐based assay. Cytokine levels in serum were measured by multiplex cytokine assay. Our data show that cells from TB patients have an increased percentage of peripheral blood CD8+αβ+ T (p = 0.02) and CD56+CD8+ T (p = 0.02) and a decreased frequency of NKG2D+CD8+ T (p = 0.02) compared with healthy donors. Unlike CD8+ T cells from healthy donors, CD8+ T cells from TB patients exhibit greater cytotoxicity, mediated by HLA class I molecules, on autologous monocytes in the presence of mycobacterial antigens (p = 0.005). Finally, TB patients have a proinflammatory profile characterized by serum high level of TNF‐α (p = 0.02) and IL‐8 (p = 0.0001), but, interestingly, IL‐4 (p = 0.002) was also increased compared with healthy donors. Our data show evidence regarding the highly cytotoxic status of CD8+ T cells in Mtb infection. These cytotoxic cells restricted to HLA‐A, B, and C could be used to optimize strategies for designing new TB vaccines or for identifying markers of disease progression.

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Section: Discussionmentioning

confidence: 99%

Tuberculosis patients display a high proportion of CD8⁺ T cells with a high cytotoxic potential

Chávez‐Galán

Illescas‐Eugenio

Álvarez-Sekely

et al. 2019

Microbiology and Immunology

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“…Only 4 of the reports took into account the study origin and population's ethnicity as a confounding factor, and these were evaluated by self-reported ethnicity [28,38,48,52].…”

Section: Journal Of Immunology Researchmentioning

confidence: 99%

Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review

Herrera

Vera

Keynan

et al. 2020

Journal of Immunology Research

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Background. Immune parameters (IP) have been extensively studied to distinguish between latent tuberculosis (LTBI) and active tuberculosis (TB). Objective. To determine the IP associated with LTBI, compared to active TB and individuals not infected by M. tuberculosis published in literature. Methods. We conducted a systematic search using Google Scholar and PubMed databases, combining the MeSH terms latent tuberculosis, Mycobacterium tuberculosis, cytokines, and biological markers, with the free terms, biomarkers and cytokines. Spanish, English, and Portuguese articles comparing the concentration of IP associated with LTBI, either in plasma/serum or in vitro, in adults and nonimmunocompromised versus individuals with TB or without M. tuberculosis infection between 2006 July and 2018 July were included. Two blinded reviewers carried out the searches, read the abstracts, and selected the articles for analysis. Participants’ information, diagnostic criteria, IP, detection methods, and biases were collected. Results. We analyzed 36 articles (of 637 abstracts) with 93 different biomarkers in different samples. We found 24 parameters that were increased only in active TB (TGF-α, CSF3, CSF2, CCL1 [I-309], IL-7, TGF-β1, CCL3 [MIP-1α], sIL-2R, TNF-β, CCL7 [MCP-3], IFN-α, fractalkine, I-TAG, CCL8 [MCP-2], CCL21 [6Ckine], PDGF, IL-22, VEGF-A, LXA4, PGE2, PGF2α, sCD163, sCD14, and 15-Epi-LXA4), five were elevated in LTBI (IL-5, IL-17F, IL-1, CCL20 [MIP-3α], and ICAM-1), and two substances were increased among uninfected individuals (IL-23 and basic FGF). We found high heterogeneity between studies including failure to account for the time/illness of the individuals studied; varied samples and protocols; different clinical classification of TB; different laboratory methods for IP detection, which in turn leads to variable units of measurement and assay sensitivities; and selection bias regarding TST and booster effect. None of the studies adjusted the analysis for the effect of ethnicity. Conclusions. It is mandatory to harmonize the study of immune parameters for LTBI diagnosis. This systematic review is registered with PROSPERO CRD42017073289.

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“…utilized an Ab array to investigate tuberculosis (TB), a disease resulting from infection by the bacterium Mycobacterium tuberculosis . They identified numerous protein biomarkers that were differentially up‐regulated in latent versus active TB infection patients, yielding an invaluable diagnostic set of protein biomarkers for early detection of the disease . In another study, Vigil et al.…”

Section: Investigation Into Disease Biology Via Proteomics Approachesmentioning

confidence: 99%

Quantitative proteomic analyses in blood: A window to human health and disease

Whittaker

Burgess

Jones

et al. 2019

Journal of Leukocyte Biology

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This review discusses how the measurement of proteins in blood and its components via quantitative proteomics analyses can inform health status. Various external and internal factors such as environmental conditions, genetic background, nutrition, diet, and lifestyle, chronic pathological conditions, disease state, or therapeutic intervention will be investigated and their effects on the protein profile will be shown. The resulting changes to ones’ health and how this protein expression information can be used in early screening/diagnostic applications, drug discovery, precision treatment, patient management, and monitoring overall health status will also be presented.

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Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array

Cited by 29 publications

References 39 publications

Tuberculosis patients display a high proportion of CD8⁺ T cells with a high cytotoxic potential

Tuberculosis patients display a high proportion of CD8⁺ T cells with a high cytotoxic potential

Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review

Quantitative proteomic analyses in blood: A window to human health and disease

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Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array

Cited by 29 publications

References 39 publications

Tuberculosis patients display a high proportion of CD8+ T cells with a high cytotoxic potential

Tuberculosis patients display a high proportion of CD8+ T cells with a high cytotoxic potential

Gaps in Study Design for Immune Parameter Research for Latent Tuberculosis Infection: A Systematic Review

Quantitative proteomic analyses in blood: A window to human health and disease

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Product

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Tuberculosis patients display a high proportion of CD8⁺ T cells with a high cytotoxic potential

Tuberculosis patients display a high proportion of CD8⁺ T cells with a high cytotoxic potential