Profiling T cell receptor β‐chain in responders after immunization with recombinant hepatitis B vaccine

Abstract

Background: T cells with edited T cell receptor β-chain variable (TRBV) are involved in the immune response to recombinant hepatitis B surface antigen (rHBsAg) vaccine and the production of hepatitis B surface antibody (HBsAb). The immune repertoire (IR) profile and mechanism of vaccination positive responders (VPR) with rHBsAg are not fully understood.Methods: The IR of six VPRs (HBsAb+, HbsAg-) with rHBsAg vaccination was established by the high throughput sequencing technique and bioinformatics analysis and compared with those in five vaccination negative responders (VNRs) (HbsAb-, HbsAg-) who were also inoculated with rHBsAg. The repertoire features of the BV, BJ and V (CDR3) J genes and immune diversity in peripheral blood mononuclear cells, respectively, were analyzed for each subject.Results: There was no significant difference in sequencing amplification indices of each sample. However, TRBV15/BJ2-3 demonstrated significantly high expression levels in VPR compared to those in the VNR group (both p < 0.05). Further results showed that the BV15/BJ2-5 level was significantly increased for VPR compared to that of VNR group. Interestingly, the motif of CDR3 in TRBV15/BJ2-5 was mostly expressed as "GGETQ" or "GETQ". Additionally, there was no remarkable difference between the two groups of distribution with respect to the different clone expression levels of V (CDR3) J. Conclusions:The features of IR in the VPR and VNR will contribute to the exploration of the mechanism of the positive response to rHBsAg, and also contribute to development of optimized hepatitis B vaccine, in addition to providing a partial interpretation of the VNR who has a relatively low infection with HBV.

Characterization of the TCR β Chain Repertoire in Peripheral Blood from Hepatitis B Vaccine Responders and Non-Responders

“… 29 In our previous study, we reported the diversity and distribution characteristics of the IR (TRB) based on the V(CDR3)J of TRBV with small cases, and there are no significant differences between the subjects with and without responding to HepB vaccination. 18 However, the Shannon index based on CDR3 is significantly higher in the HepB vaccine responders compared to that in non-responders. Similarly, the CF100 is significantly lower in responders.…”

“…Subsequently, the raw sequence was screened, and the low quality sequences were deleted, the sequencing background was filtered 17 and the international ImMunoGeneTics (IMGT) database ( www.imgt.org ) was used as standard reference sequences and the sequences obtained were compared with the IMGT database in the present study, please refer to our previous report for more detailed steps. 18 …”

Effects of hepatitis B virus infection and strategies for preventing mother-to-child transmission on maternal and fetal T-cell immunity

“…According to current research, the recipient’s response to the hepatitis B vaccine may be influenced by their T-cell immunity. For instance, the edited T-cell receptor-chain variable was linked to the immune response of healthy vaccinees to recombinant hepatitis B surface antigen (rHBsAg) as well as the production of hepatitis B surface antibody (HbsAb) ( 88 ). Circulating follicular helper T cells (cTfh) and subsets were shown to be involved in the immune response to hepatitis B vaccine injection in vitro , and the CXCR3-Tfh cell subset was preferentially activated when HBsAg stimulated PBMCs.…”

Folic acid supplementation in pregnant women with hepatitis B surface antigen improves infant hepatitis B surface antibody mediated by infant IL-4

“…Previous work has shown that FAS is associated with an increase in levels of markers related to immunity, such as B lymphocyte response markers, innate immune proinflammatory pathways (14) , antigen processing and presentation markers, nuclear factor-k-gene binding signalling pathways, tumor necrosis factor signalling (15) , interleukin-8 (16) and interleukin-4 (IL-4) (17,18) . Previous research has also revealed a positive association between immune markers and hepatitis B vaccine response, including T-cell receptor β-chain variable (19) , NDRG1-expressing myeloid dendritic cell-2, CDKN1C-expressing myeloid dendritic cell-4 (20) , chemokine (C-X-C motif) ligand 12, https://doi.org/10.1017/S000711452200229X Published online by Cambridge University Press Accepted manuscript interleukin-27 (21) , interferon-γ, interleukin-2 (22) , interleukin-17, interleukin-18 (23) and IL-4 (22,24,25) . Among the above immune markers, we noticed that IL-4 is related to both FAS and hepatitis B vaccine response.…”