Profiling of the Early Nitrogen Stress Response in the Diatom <i>Phaeodactylum tricornutum</i> Reveals a Novel Family of RING-Domain Transcription Factors

Matthijs, Michiel; Fabris, Michele; Broos, Stefan; Vyverman, Wim; Goossens, Alain

doi:10.1104/pp.15.01300

Cited by 44 publications

(40 citation statements)

References 48 publications

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“…MapMan analysis of the nitrogen starvation response (Fig A) corroborated our reported findings (Matthijs et al , ), indicating that carbon metabolism was strongly affected, including gluconeogenesis, glycolysis, and the other pathways represented in Fig A. Notably, although nitrogen‐starved diatoms accumulated lipids, no clear upregulation of lipid biosynthetic genes or any co‐expression cluster of lipid metabolism or recycling genes was detected.…”

Section: Resultssupporting

confidence: 88%

The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum

et al. 2017

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Diatoms are amongst the most important marine microalgae in terms of biomass, but little is known concerning the molecular mechanisms that regulate their versatile metabolism. Here, the pennate diatom was studied at the metabolite and transcriptome level during nitrogen starvation and following imposition of three other stresses that impede growth. The coordinated upregulation of the tricarboxylic acid (TCA) cycle during the nitrogen stress response was the most striking observation. Through co-expression analysis and DNA binding assays, the transcription factor bZIP14 was identified as a regulator of the TCA cycle, also beyond the nitrogen starvation response, namely in diurnal regulation. Accordingly, metabolic and transcriptional shifts were observed upon overexpression of in transformed cells. Our data indicate that the TCA cycle is a tightly regulated and important hub for carbon reallocation in the diatom cell during nutrient starvation and that bZIP14 is a conserved regulator of this cycle.

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Section: Resultssupporting

confidence: 88%

The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum

et al. 2017

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“…Increased N-acquisition transcripts in N-deprived cultures. It is well documented that many organisms, including the green alga Chlamydomonas reinhardtii [11][12][13][14] and the diatom Phaeodactylum tricornutum 15,16 , show increased accumulation of transcripts involved in N acquisition when limited for N. RT-qPCR was used to determine if the N-associated transcripts upregulated in hospite were also upregulated when free-living SSB01 cells growing in a minimal but N-replete medium (Daigo's IMK) were shifted to IMK medium lacking N. In all cases tested but one, the transcript levels increased in the IMK-N culture but not in the control IMK culture, with increases as large as 12-fold ( Fig. 2; Supplementary Fig.…”

Section: Resultsmentioning

confidence: 99%

Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations

et al. 2020

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In cnidarian-Symbiodiniaceae symbioses, algal endosymbiont population control within the host is needed to sustain a symbiotic relationship. However, the molecular mechanisms that underlie such population control are unclear. Here we show that a cnidarian host uses nitrogen limitation as a primary mechanism to control endosymbiont populations. Nitrogen acquisition and assimilation transcripts become elevated in symbiotic Breviolum minutum algae as they reach high-densities within the sea anemone host Exaiptasia pallida. These same transcripts increase in free-living algae deprived of nitrogen. Symbiotic algae also have an elevated carbon-to-nitrogen ratio and shift metabolism towards scavenging nitrogen from purines relative to free-living algae. Exaiptasia glutamine synthetase and glutamate synthase transcripts concomitantly increase with the algal endosymbiont population, suggesting an increased ability of the host to assimilate ammonium. These results suggest algal growth and replication in hospite is controlled by access to nitrogen, which becomes limiting for the algae as their population within the host increases.

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“…There has been progress in increasing the lipid biosynthesis capability of microalgae through high biomass cultivation, metabolic engineering and genetic engineering [6–8]. To increase fatty acid (FA) biosynthesis, genetic engineering regulating cell metabolic pathways is feasible, including the over-expression or inhibition of certain rate-limiting enzymes [7–9], the comprehensive regulation of lipid biosynthesis by transcription factors [10], the construction of genes in association with spontaneous secretion of FA and the construction of efficient expression vectors for exogenous genes [3]. …”

Section: Introductionmentioning

confidence: 99%

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

Wei

Huang

2017

PLoS ONE

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Microalga Nannochloropsis oculata is a promising alternative feedstock for biodiesel. Elevating its oil-yielding capacity is conducive to cost-saving biodiesel production. However, the regulatory processes of multi-factor collaborative stresses (MFCS) on the oil-yielding performance of N. oculata are unclear. The duration effects of MFCS (high irradiation, nitrogen deficiency and elevated iron supplementation) on N. oculata were investigated in an 18-d batch culture. Despite the reduction in cell division, the biomass concentration increased, resulting from the large accumulation of the carbon/energy-reservoir. However, different storage forms were found in different cellular storage compounds, and both the protein content and pigment composition swiftly and drastically changed. The analysis of four biodiesel properties using pertinent empirical equations indicated their progressive effective improvement in lipid classes and fatty acid composition. The variation curve of neutral lipid productivity was monitored with fluorescent Nile red and was closely correlated to the results from conventional methods. In addition, a series of changes in the organelles (e.g., chloroplast, lipid body and vacuole) and cell shape, dependent on the stress duration, were observed by TEM and LSCM. These changes presumably played an important role in the acclimation of N. oculata to MFCS and accordingly improved its oil-yielding performance.

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Profiling of the Early Nitrogen Stress Response in the Diatom Phaeodactylum tricornutum Reveals a Novel Family of RING-Domain Transcription Factors

Cited by 44 publications

References 48 publications

The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum

The transcription factor bZIP14 regulates the TCA cycle in the diatom Phaeodactylum tricornutum

Symbiont population control by host-symbiont metabolic interaction in Symbiodiniaceae-cnidarian associations

Long-duration effect of multi-factor stresses on the cellular biochemistry, oil-yielding performance and morphology of Nannochloropsis oculata

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