Profiling of secondary metabolites and DNA typing of three different Annona cultivars grown in Egypt

Abstract

Objectives Natural products are often efficacious and safe alternatives to synthetic drugs. This study explored secondary leaves and bark metabolites profiles in extracts of a new Egyptian hybrid, Annona cherimola × Annona squamosa, known as Abdel Razek. This hybrid exhibited 100% similarity with A. cherimola as evidenced by random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analyses. Methods Primary constituents in methanol extracts of different plant organs were identified. Extracts richest in alkaloids and polyphenolics were assessed for in vitro antioxidant activity and the most potent were further studied in vivo for treating gastric ulcer in rats. The latter activity was assessed histopathologically. Results Structural analysis with HPLC/ESI-MSn, and UPLC/HESI-MS/MS identified 63 metabolites, including seven amino acids, 20 alkaloids, 16 flavonoids, eight phenolics and other compounds. Severe stomach alteration was observed after ethanol induction in rats. Ulcer score, oxidative stress biomarkers, cell organelles biomarker enzymes, and gastrointestinal histological features improved to variable degrees after treatment with Annona Abdel Razek hybrid leaves and bark methanol extracts. Conclusion Extracts of Annona Abdel Razek had showed in vitro antioxidant effect and may be promising for the treatment of gastric ulcers. Graphical abstract

Comprehensive Tools of Alkaloid/Volatile Compounds–Metabolomics and DNA Profiles: Bioassay-Role-Guided Differentiation Process of Six Annona sp. Grown in Egypt as Anticancer Therapy

“…The trees were identified by Therese Labib, a specialized botanical consultant, and Dr. M. A. Gibali, from the Taxonomy Department at the Faculty of Science, Cairo University. The voucher specimens were stored at the Herbarium of the National Research Centre [ 18 ]. The aerial component (leaves and stem) was obtained from a private farm in Giza, Egypt, for the metabolomic analysis, while immature leaves were collected for DNA fingerprinting ( Table 1 ).…”

“…A. abdel-razek is an Egyptian cultivar resulting from a cross between A. cherimola and A. squamosa . Recently, it has also been cultivated in other Arabian countries including Sudan, Lebanon, Oman, Kuwait, Palestine, and Jordan [ 18 ].…”

A comprehensive tool in recycling plant-waste of Gossypium barbadense L agricultural and industrial waste extracts containing gossypin and gossypol: hepatoprotective, anti-inflammatory and antioxidant effects

“…The separation was performed with an Ascentis ® C18 Column (4.6 × 150 mm, 3 µm). The mobile phases consisted of A: 0.1% formic acid; B: acetonitrile (LC grade) [ 22 ]. The mobile phase gradient was programmed as follows: 10% B at 0–1 min, 10–90% B from 1 to 33 min, 90% B from 33 to 37 min, 10% at 37.1, 10% from 37.1 to 40 min.…”

Fighting cytokine storm and immunomodulatory deficiency: By using natural products therapy up to now

“…Curcumin, piperine, luteolin, phenylnaphthalene, withanolides, anonaine, resveratrol, emodin, anthrarufin, and caffeine are natural immunomodulatory compounds obtained from plant sources known to limit the generation and secretion of inflammatory cytokines/chemokines ( Hamed et al, 2019 ; El-Gengaihi et al, 2020a ; Rolta et al, 2021a ; Rolta et al, 2021b ; Mohammed et al, 2022b ; Mohammed et al, 2022c ; Rolta et al, 2022 ). This inhibitory impact is mediated through analysis and planning involved in the generation and release of cytokines and chemokines (e.g., NF-κB, JAK/STAT, and MAPK/ERK).…”