Profiling of phenolic compounds and antioxidant activity of Melia azedarach L. leaves and fruits at two stages of maturity

M’Rabet, Yassine; Rokbeni, Nesrine; Cluzet, Stéphanie; Boulila, Abdennacer; Richard, Tristan; Krisa, Stéphanie; Marzouki, Lamjed; Casabianca, Hervé; Hosni, Karim

doi:10.1016/j.indcrop.2017.05.048

Cited by 26 publications

(15 citation statements)

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“…Compounds of the same class as detected in P. dulce have also been reported previously in this same species [70], in other plants [71][72][73] for which AA has been demonstrated in vitro [32,74,75], and even specifically in H. contortus [75,76]. However, glycosyl flavonoids and their aglycones were shown to have the opposite effects on gastrointestinal nematodes.…”

Section: Discussionsupporting

confidence: 80%

In Vitro Anthelmintic Evaluation of Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce: Fingerprint Analysis of Extracts by UHPLC-Orbitrap Mass Spectrometry

et al. 2020

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In the present work, the anthelmintic activity (AA) of ethanolic extracts obtained from Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce was evaluated using the third-stage-larval (L3) exsheathment inhibition test (LEIT) and egg hatch test (EHT) on Haemonchus contortus. Extracts were tested at concentrations of 0.3, 0.6, 1.2, 2.5, 5.0, 10, 20, and 40 mg/mL. The larval exsheathment inhibition (LEI) results showed that G. sepium achieved the highest average inhibition of 91.2%, compared with 44.6% for P. dulce and 41.0% for L. leucocephala at a concentration of 40 mg/mL; the corresponding IC50 values were 22.4, 41.7, and 43.3 mg/mL, respectively. The rates of egg hatching inhibition (EHI) at a concentration of 5 mg/mL were 99.5% for G. sepium, 64.2% for P. dulce, and 54% for L. leucocephala; the corresponding IC50 values were 1.9 mg/mL for G. sepium, 3.9 mg/mL for P. dulce, and 4.3 mg/mL for L. leucocephala. The species extracts studied here were also analyzed by ultra-high performance liquid chromatography and Orbitrap high resolution mass spectrometry (UHPLC-Q/Orbitrap/MS/MS), resulting in the compounds’ identification associated with AA. Glycosylated flavonoids and methoxyphenols were observed in all three species: fatty acids in G. sepium and P. dulce; phenylpropanoids, anthraquinone glycosides, amino acids and glycosylated phenolic acids in G. sepium; and flavonoids in L. leucocephala. Comparatively, G. sepium presented a greater diversity of compounds potentially active against the control of gastrointestinal nematodes, which was associated with the results obtained in the applied tests.

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Section: Discussionsupporting

confidence: 80%

In Vitro Anthelmintic Evaluation of Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce: Fingerprint Analysis of Extracts by UHPLC-Orbitrap Mass Spectrometry

et al. 2020

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“…Previous article regarding total phenolic content of M. azedarach leaves reported different results. Total phenolic content of leaves extract reported by M'rabet (2017) [13] was 59.1 mg GAE/g and 50.01 mg GAE/g in an earlier study conducted by Kaneria (2009) [26] . Observed differences could be explained by geographical and climatic factors.…”

Section: Resultsmentioning

confidence: 99%

“…CUPRAC and FRAP assays were evaluated the electron‐donating ability of the extract and the results were 109.89 mg and 65.02 mg TE/g, respectively. According to literature search, several articles investigate antioxidant properties of M. azedarach parts (leaves, stem, or fruits) [13,15,26] . In these articles, the extract exhibited significant antioxidant effects that can be correlated with compound, like flavonoids and hydroxy cinnamic acids, with antioxidant properties [29,30] …”

Section: Resultsmentioning

confidence: 99%

“…The chemical composition of M. azedarach is highly complex and have been subject to several investigations aimed to evaluate the presence of bioactive constituents [7,11–17] . Previous article revealed that leaves contains phenylpropanoids and flavonoids like myricetin, quercetin, kaempferol and isorhamnetin derivatives [13] . Previous studies on the genus Melia have led to the isolation of a variety of structurally diverse compounds including triterpenoids, steroids, and limonoids.…”

Section: Introductionmentioning

confidence: 99%

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Phytochemical Investigations and In Vitro Bioactivity Screening on Melia azedarach L. Leaves Extract from Nepal

Shrestha

Ferrarese

Sut

et al. 2021

Chemistry & Biodiversity

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Melia azedarach is a common tree used in the traditional medicine of Nepal. In this work, leaves were considered as source of bioactive constituents and composition of methanol extract was evaluated and compared with starting plant material. Flavonoid glycosides and limonoids were identified and quantified by HPLC‐DAD‐MSn approaches in dried leaves and methanolic extract, while HPLC‐APCI‐MSn and GC/MS analysis were used to study phytosterol and lipid compositions. β‐Sitosterol and rutin were the most abundant constituents. HPLC‐APCI‐MSn and HPLC‐DAD‐MSn analysis revealed high levels of phytosterols and flavonoids in methanolic extract accounting 9.6 and 7.5 % on the dried weight, respectively. On the other hand, HPLC/MSn data revealed that limonoid constituents were in minor amount in the extract <0.1 %, compared with leaves (0.7 %) indicating that degradation occurred during extraction or concentration procedures. The methanol extract was subjected to different bioassays, and antioxidant activity was evaluated. Limited inhibitory activity on acetyl and butyryl cholinesterase, as well as on amylase were detected. Moreover, tyrosinase inhibition was significant resulting in 131.57±0.51 mg kojic acid equivalents/g of dried methanol extract, suggesting possible use of this M. azedarach extract in skin hyperpigmentation conditions. Moderate cytotoxic activity, with IC50 of 26.4 μg/mL was observed against human ovarian cancer cell lines (2008 cells). Our findings indicate that the Nepalese M. azedarach leaves can be considered as valuable starting material for the extraction of phenolics and phytosterols, yielding extracts with possible cosmetic and pharmaceutical applications.

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“…The ingredient components active against Meloidogyne incognita were identified as p -coumaric acid and p -hydroxybenzoic acid [ 97 ]. At a wavelength of 280 nm, the main phenolic compounds in the leaves of Melia azedarach were found to be rutin, quercetin-3- O -neohesperidoside, kaempferol-3- O -rutinoside, feruloylglucaric acid, and feruloylquinic acid derivative [ 98 ]. Extracts from different parts of M. azedarach exhibited fungistatic activity against A. flavus , Diaporthe phaseolorum var.…”

Section: Resultsmentioning

confidence: 99%

Antifungal Activities of Wood and Non-Wood Kraft Handsheets Treated with Melia azedarach Extract Using SEM and HPLC Analyses

et al. 2021

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The main objective of this work was to evaluate pulp produced by kraft cooking for wood materials (WMT) (Bougainvillea spectabilis, Ficus altissima, and F. elastica) and non-wood materials (NWMT) (Sorghum bicolor and Zea mays stalks) and to study the fungal activity of handsheets treated with Melia azedarach heartwood extract (MAHE) solutions. Through the aforementioned analyses, the ideal cooking conditions were determined for each raw material based on the lignin percentage present. After cooking, pulp showed a decrease in the Kappa number produced from WMT, ranging from 16 to 17. This was in contrast with NWMT, which had Kappa numbers ranging from 31 to 35. A difference in the optical properties of the pulp produced from WMT was also observed (18 to 29%) compared with pulp produced from NWMT (32.66 to 35.35%). As for the evaluation of the mechanical properties, the tensile index of the pulp ranged from 30.5 to 40 N·m/g for WMT and from 44.33 to 47.43 N·m/g for NWMT; the tear index ranged from 1.66 to 2.55 mN·m2/g for WMT and from 4.75 to 5.87 mN·m2/g for NWMT; and the burst index ranged from 2.35 to 2.85 kPa·m2/g for WMT and from 3.92 to 4.76 kPa·m2/g for NWMT. Finally, the double fold number was 3 compared with that of pulp produced from pulp, which showed good values ranging from 36 to 55. In the SEM examination, sheets produced from treated handsheets with extract from MAHE showed no growth of Aspergillus fumigatus over paper discs manufactured from B. speclabilis pulp wood. Pulp paper produced from Z. mays and S. bicolor stalks was treated with 1% MAHE, while pulp paper from F. elastica was treated with 0.50% and 1% MAHE. With the addition of 0.5 or 1% MAHE, Fusarium culmorum showed no increase in growth over the paper manufactured from B. speclabilis, F. altissima, F. elastica and Zea mays pulps with visual inhibition zones found. There was almost no growth of S. solani in paper discs manufactured from pulps treated with 1% MAHE. This is probably due to the phytochemical compounds present in the extract. The HPLC analysis of MAHE identified p-hydroxybenzoic acid, caffeine, rutin, chlorogenic acid, benzoic acid, quinol, and quercetin as the main compounds, and these were present in concentrations of 3966.88, 1032.67, 834.13, 767.81, 660.64, 594.86, and 460.36 mg/Kg extract, respectively. Additionally, due to the importance of making paper from agricultural waste (stalks of S. bicolor and Z. mays), the development of sorghum and corn with high biomass is suggested.

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Profiling of phenolic compounds and antioxidant activity of Melia azedarach L. leaves and fruits at two stages of maturity

Cited by 26 publications

References 53 publications

In Vitro Anthelmintic Evaluation of Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce: Fingerprint Analysis of Extracts by UHPLC-Orbitrap Mass Spectrometry

In Vitro Anthelmintic Evaluation of Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce: Fingerprint Analysis of Extracts by UHPLC-Orbitrap Mass Spectrometry

Phytochemical Investigations and In Vitro Bioactivity Screening on Melia azedarach L. Leaves Extract from Nepal

Antifungal Activities of Wood and Non-Wood Kraft Handsheets Treated with Melia azedarach Extract Using SEM and HPLC Analyses

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