Profiling of indole metabolic pathway in thermo-sensitive Bainong male sterile line in wheat (Triticum aestivum L.)

Su, Qing; Yang, Jing; Fu, Qing Yun; Jia, Fei Yun; Li, Suoping; Li, Yong; Li, You Yong

doi:10.1007/s12298-018-0626-0

Cited by 8 publications

(7 citation statements)

References 22 publications

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“…Among auxin signal transduction-related genes, homologs of auxin response factor (ARF) genes and several auxin biosynthesis-related genes expressed less in D-ST. Reduced expression of auxin biosynthesis and signal transduction related genes were closely related to male sterility ( Su et al, 2019 ). Abnormality of auxin homeostasis might be a major factor leading to the phenotype of dms .…”

Section: Resultsmentioning

confidence: 99%

Cytological and molecular characterizations of a novel 2A nullisomic line derived from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility

Jiao

Zhu

et al. 2020

PeerJ

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A dwarf, multi-pistil and male sterile dms mutant was previously reported by us. However, the genetic changes in this dms are unclear. To examine the genetic changes, single nucleotide polymorphism (SNP) association, chromosome counting, and high-resolution chromosome fluorescence in situ hybridization (FISH) techniques were employed. By comparing tall plants (T) with dwarf plants (D) in the offspring of dms mutant plants, SNP association analysis indicated that most SNPs were on chromosome 2A. There were three types in offspring of dms plants, with 42, 41 and 40 chromosomes respectively. High-resolution chromosome painting analysis demonstrated that T plants had all 42 wheat chromosomes; the medium plants (M) had 41 chromosomes, lacking one chromosome 2A; while D plants had 40 wheat chromosomes, and lacked both 2A chromosomes. These data demonstrated that dms resulted from a loss of chromosome 2A. We identified 23 genes on chromosome 2A which might be involved in the development of stamens or pollen grains. These results lay a solid foundation for further analysis of the molecular mechanisms of wheat male sterility. Because D plants can be used as a female parent to cross with other wheat genotypes, dms is a unique germplasm for any functional study of chromosome 2A and wheat breeding specifically targeting genes on 2A.

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Section: Resultsmentioning

confidence: 99%

Cytological and molecular characterizations of a novel 2A nullisomic line derived from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility

Jiao

Zhu

et al. 2020

PeerJ

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“…The effect of IAA on plant male sterility was often reported. The reduction of the express of IAA related gene in wheat was associated with the occurrence of male-sterility ( Su et al., 2019 ). At the third stage, the contents of IAA, GA 3 and ZR in CMS were remarkably lower than its maintainer of Chinese Cabbage ( Liu et al., 2014 ).…”

Section: Discussionmentioning

confidence: 99%

A male-sterile mutant with necrosis-like dark spots on anthers was generated in cotton

Zhang

et al. 2023

Front. Plant Sci.

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Although conventional hybrid breeding has paved the way for improving cotton production and other properties, it is undoubtedly time and labor consuming, while the cultivation of male sterile line can fix the problem. Here, we induced male sterile mutants by simultaneously editing three cotton EXCESS MICROSPOROCYTES1 (GhEMS1) genes by CRISPR/Cas9. Notably, the GhEMS1 genes are homologous to AtEMS1 genes, which inhibit the production of middle layer and tapetum cells as well, leading to male sterility in cotton. Interestingly, there are necrosis-like dark spots on the surface of the anthers of GhEMS1s mutants, which is different from AtEMS1 mutant whose anther surface is clean and smooth, suggesting that the function of EMS1 gene has not been uncovered yet. Moreover, we have detected mutations in GhEMS1 genes from T0 to T3 mutant plants, which had necrosis-like dark spots as well, indicating that the mutation of the three GhEMS1 genes could be stably inherited. Dynamic transcriptomes showed plant hormone pathway and anther development genetic network were differential expression in mutant and wild-type anthers. And the lower level of IAA content in the mutant anthers than that in the wild type at four anther developmental stages may be the reason for the male sterility. This study not only facilitates the exploration of the basic research of cotton male sterile lines, but also provides germplasms for accelerating the hybrid breeding in cotton.

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“…Even at very low concentrations, phytohormones are believed to regulate a variety of cellular processes in plants, and play critical roles and coordinate various pathways in signal transduction during an abiotic-stress response [44].…”

Section: Effects Of the Drought Response On Phytohormone Signalingmentioning

confidence: 99%

Comparative Transcriptome Analysis of Two Aegilops tauschii with Contrasting Drought Tolerance by RNA-Seq

Zhao

Bai

et al. 2020

IJMS

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As the diploid progenitor of common wheat, Aegilops tauschii is considered to be a valuable resistance source to various biotic and abiotic stresses. However, little has been reported concerning the molecular mechanism of drought tolerance in Ae. tauschii. In this work, the drought tolerance of 155 Ae. tauschii accessions was firstly screened on the basis of their coleoptile lengths under simulated drought stress. Subsequently, two accessions (XJ002 and XJ098) with contrasting coleoptile lengths were selected and intensively analyzed on rate of water loss (RWL) as well as physiological characters, confirming the difference in drought tolerance at the seedling stage. Further, RNA-seq was utilized for global transcriptome profiling of the two accessions seedling leaves under drought stress conditions. A total of 6969 differentially expressed genes (DEGs) associated with drought tolerance were identified, and their functional annotations demonstrated that the stress response was mediated by pathways involving alpha-linolenic acid metabolism, starch and sucrose metabolism, peroxisome, mitogen-activated protein kinase (MAPK) signaling, carbon fixation in photosynthetic organisms, and glycerophospholipid metabolism. In addition, DEGs with obvious differences between the two accessions were intensively analyzed, indicating that the expression level of DEGs was basically in alignment with the physiological changes of Ae. tauschii under drought stress. The results not only shed fundamental light on the regulatory process of drought tolerance in Ae. tauschii, but also provide a new gene resource for improving the drought tolerance of common wheat.

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Profiling of indole metabolic pathway in thermo-sensitive Bainong male sterile line in wheat (Triticum aestivum L.)

Cited by 8 publications

References 22 publications

Cytological and molecular characterizations of a novel 2A nullisomic line derived from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility

Cytological and molecular characterizations of a novel 2A nullisomic line derived from a widely-grown wheat cultivar Zhoumai 18 conferring male sterility

A male-sterile mutant with necrosis-like dark spots on anthers was generated in cotton

Comparative Transcriptome Analysis of Two Aegilops tauschii with Contrasting Drought Tolerance by RNA-Seq

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