Profiling of dental plaque microflora on root caries lesions and the protein-degrading activity of these bacteria

Hara, Kazuhiro; Sato, Takuichi; Shimauchi, Hidetoshi; Takahashi, Nana

doi:10.1007/978-4-431-99644-6_63

Cited by 21 publications

(29 citation statements)

References 3 publications

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“…P. acidifaciens, which is a Gram-positive anaerobic bacillus, is reported to be saccharolytic, producing large amounts of acetic and propionic acids [Downes and Wade, 2009]. Propionibacteria are increasingly reported to be present in deep dentin [Edwardsson, 1974;Hoshino, 1985;Munson et al, 2004;Chhour et al, 2005;Aas et al, 2008;Downes and Wade, 2009;Belda-Ferre et al, 2011] and root caries [Hashimoto et al, 2011]. Recently, Chhour et al [2005] reported that obligate anaerobes dominate carious dentin lesions.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Biofilm Composition in Caries and Caries-Free Subjects

Wolff

Frese²,

Maier-Kraus³

et al. 2012

Caries Res

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Background: Certain major pathogens such as Streptococcus mutans, Lactobacillus spp. and others have been reported to be involved in caries initiation and progression. Yet, in addition to those leading pathogens, microbial communities seem to be much more diverse and individually differing. The aim of this study, therefore, was to analyze the bacterial composition of carious dentin and the plaque of caries-free patients by using a custom-made, real-time quantitative polymerase chain reaction assay (RQ-PCR). Methods: The study included 26 patients with caries and 28 caries-free controls. Decayed tooth substance and plaque samples were harvested. Bacterial DNA was extracted and tested for the presence of 43 bacterial species or species groups using RQ-PCR. Results: Relative quantification revealed that Propionibacterium acidifaciens was significantly more abundant in caries samples than were other microorganisms (fold change 169.12, p = 0.023). In the caries-free samples, typical health-associated species were significantly more prevalent. Unsupervised hierarchical cluster analysis showed a high abundance of P. acidifaciens in caries subjects and distinct but individually differing bacterial clusters in the caries-free subjects. The distribution of 11 bacteria allowed full discrimination between caries and caries-free subjects. Conclusion: Within the investigated cohort, P. acidifaciens was the only pathogen significantly more abundant in caries subjects. Cluster analysis yielded a diverse flora in caries-free subjects, whereas it was narrowed down to a small range of a few outcompeting members in caries subjects.

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Section: Discussionmentioning

confidence: 99%

Bacterial Biofilm Composition in Caries and Caries-Free Subjects

Wolff

Frese²,

Maier-Kraus³

et al. 2012

Caries Res

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“…Biofilm accumulation slows the diffusion of organic acids and restricts the access of pH-buffering saliva, subjecting the enamel to repeated and prolonged exposure to an acidic pH (pH Ͻ5.5) and thereby initiating the dental caries process (3,14). Left untreated, further demineralization of the enamel and extension of the lesion expose the underlying dentin, presenting collagen and other, additional substrates for bacterial colonization (15,16). Other oral tissues, such as the cementum, root, and periodontal ligament fibers, are also rich in collagen (17) and, if exposed to the oral environment, may be vulnerable to attachment and colonization by microbes equipped with collagen-binding adhesins (13,(18)(19)(20).…”

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confidence: 99%

The Collagen Binding Protein Cnm Contributes to Oral Colonization and Cariogenicity of Streptococcus mutans OMZ175

et al. 2015

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f Streptococcus mutans is the etiological agent of dental caries and one of the many bacterial species implicated in infective endocarditis. The expression of the collagen-binding protein Cnm by S. mutans has been associated with extraoral infections, but its relevance for dental caries has only been theorized to date. Due to the collagenous composition of dentinal and root tissues, we hypothesized that Cnm may facilitate the colonization of these surfaces, thereby enhancing the pathogenic potential of S. mutans in advancing carious lesions. As shown for extraoral endothelial cell lines, Cnm mediates the invasion of oral keratinocytes and fibroblasts by S. mutans. In this study, we show that in the Cnm ؉ native strain, OMZ175, Cnm mediates stringent adhesion to dentinal and root tissues as well as collagen-coated surfaces and promotes both cariogenicity and carriage in vivo. In vitro, ex vivo, and in vivo experiments revealed that while Cnm is not universally required for S. mutans cariogenicity, it contributes to (i) the invasion of the oral epithelium, (ii) enhanced binding on collagenous surfaces, (iii) implantation of oral biofilms, and (IV) the severity of caries due to a native Cnm ؉ isolate. Taken together, our findings reveal that Cnm is a colonization factor that contributes to the pathogenicity of certain S. mutans strains in their native habitat, the oral cavity.

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“…Nosotros hemos demostrado un rol protector contra la desmineralización inducida por Streptococcus mutans por parte de algunos ácidos grasos (28) . Las proteínas aportadas en forma exógena en la dieta pueden ser degradadas por flora microbiana proteolítica específica presente en el biofilm oral (29) . Nuestro grupo abordará el cómo la presencia de proteínas de la dieta puede condicionar un mayor riesgo de caries radiculares en pacientes diabéticos con una serie de experimentos relevantes para intentar esclarecer este tema.…”

Section: Discussionunclassified

Asociación entre el consumo de macronutrientes de la dieta y caries en adultos y adultos mayores diabéticos tipo 2

Giacaman

Quintanilla

Poblete

2012

Rev. Clin. Periodoncia Implantol. Rehabil. Oral

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RESUMEN Objetivos: Se ha reportado una mayor tasa de caries en pacientes con diabetes mellitus tipo 2 (DM), probablemente debido a cambios en los patrones de dieta. El objetivo de este estudio fue determinar si existe una asociación entre la composición de macronutrientes de la dieta y la caries en sujetos con DM. Métodos: Una muestra compuesta por 33 sujetos con DM y 37 controles sin la patología fue examinada para determinar la experiencia de caries mediante el índice COPD y la prevalencia de caries radiculares con ICDAS II. Se aplicó una encuesta de dieta de reporte de 24 horas. Para determinar la composición de la dieta, los alimentos fueron clasificados acorde al contenido de macronutrientes; carbohidratos, proteínas y lípidos (gramos/día, kilocalorías y porcentaje del nutriente/día). Resultados: Los resultados fueron analizados mediante Kruskal-Wallis y Mann-Whitney. Un análisis de correlación y un modelo de regresión lineal se utilizaron para analizar la asociación entre composición de macronutrientes y la tasa de caries coronales y radiculares, con un nivel de significancia del 95%. Resultados: Los pacientes diabéticos tipo 2 mostraron mayor número de dientes perdidos por caries, más dientes obturados y más caries radiculares (p<0.05) que los controles sin DM. Los diabéticos presentaron menor consumo de carbohidratos (p=0.021), pero mayor consumo de proteínas (p=0.0405) que los controles. Se verificó una asociación directa entre un mayor consumo de proteínas con una tasa mayor de caries radiculares en pacientes con DM (p<0.001). Conclusión: Las diferencias en el consumo de macronutrientes en pacientes con DM no se relacionan con tasas diferenciales de caries coronales, pero un mayor consumo de proteínas se asocia con una mayor prevalencia de caries radiculares. ABSTRACT Objectives: Higher caries rates have been reported for type 2 diabetes mellitus patients (DM). Changes in dietary patterns may be partly responsible. The aim of this study was to examine a potential association between dietary macronutrient composition and caries experience in DM patients. Methods: Thirty three adults with DM and thirty seven controls, not affected by DM, were examined to assess coronal caries through DMFT index and root caries by ICDAS II. A 24-hr diet recall survey was used to determine dietary macronutrient composition supplemented with standardized tables and expressed as grams /day, kilocalories/nutrient and percentage of nutrient/day. Results were compared using Kruskal-Wallis and Mann-Whitney. A correlation analysis was performed and a linear regression model was built for a potential association between macronutrient composition and coronal or root caries. A 95% significance level was set. Results: DM patients showed lower remaining teeth, more fillings and more root caries (p<0.05) than controls. Lower carbohydrate consumption (p=0.021), but higher protein intake (p=0.0405) was observed in DM patients as compared with controls. A statistically significant association between an increased protein consumption and h...

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Profiling of dental plaque microflora on root caries lesions and the protein-degrading activity of these bacteria

Cited by 21 publications

References 3 publications

Bacterial Biofilm Composition in Caries and Caries-Free Subjects

Bacterial Biofilm Composition in Caries and Caries-Free Subjects

The Collagen Binding Protein Cnm Contributes to Oral Colonization and Cariogenicity of Streptococcus mutans OMZ175

Asociación entre el consumo de macronutrientes de la dieta y caries en adultos y adultos mayores diabéticos tipo 2

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