Profiling of carbonyl compounds in serum by stable isotope labeling - Double precursor ion scan - Mass spectrometry analysis

Guo, Ning; Peng, Chunyan; Zhu, Quan-Fei; Feng, Yu‐Qi

doi:10.1016/j.aca.2017.03.006

Cited by 46 publications

(37 citation statements)

References 33 publications

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“…Such a derivatization pathway coupled with double precursor ion scan‐MS allowed the detection of 156 carbonyl compounds in human serum. The study results demonstrated elevated levels of 44 compounds in myelogenous leukemia relative to the control group (Guo, Peng, Zhu, Yuan, & Feng, ). In addition, the 13 C 0 / 13 C 2 ‐dansyl hydrazine pair was used for labeling carbonyl compounds from different classes.…”

Section: Isotope‐coded Derivatization Reagents For Lc–ms Analysismentioning

confidence: 82%

“…The 2 H 0 / 2 H 3 -HMP reagent pair was utilized for the derivatization of allopregnanolone and pregnenolone in mouse brain tissue, where 2 H 3 -HMP was synthesized from 2-chloropyridine, 2 H 4 -methanol, and trifluoromethane-sulfonic anhydride. In this derivatization methodology, the sensitivity for allopregnanolone and pregnenolone was enhanced nearly 500 and 3000 times; besides, improved accuracy and precision could be established (Higashi, Aiba, Tanaka, Yoshizawa, & Ogawa, 2016 (Guo, Peng, Zhu, Yuan, & Feng, 2017). In addition, the 13 C 0 / 13 C 2 -dansyl hydrazine pair was used for labeling carbonyl compounds from different classes.…”

Section: Hydrazine Reagentsmentioning

confidence: 99%

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Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

El-Maghrabey

Kishikawa

Kuroda

2020

Biomedical Chromatography

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Currently, LC–MS has various applications in different areas such as metabolomics, pharmacokinetics, and pathological studies. Yet, matrix effects resulting from co‐existing constituents remain a major problem for LC–MS [or LC–tandem mass spectrometry (LC–MS/MS)]. Moreover, technical problems and instrumental drifts may lead to ion abundance variance. Thus, an internal standard (IS) is required to guarantee the accuracy and precision of the method. Because of their limited number, isotope‐coded derivatization (ICD) has been recently introduced to overcome this problem. For ICD, a stable heavy isotope‐coded moiety is used for labeling the standard or the control sample and the formed products can act as ISs. A light form of the reagent is used for labeling the sample. Then, both are mixed and analyzed by LC–MS(/MS). This strategy permits the identification of different unknown analytes including potential metabolites and disease biomarkers. All these attributes lead to persistent growth in the applications of ICD LC–MS(/MS) in various biomedical branches. In this article we review the ICD methods published in the last eight years for biomedical applications as well as briefly summarize other applications for environmental and food analyses as some of their used ICD reagents were further applied for analyzing biological specimens or have the potential for that.

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Section: Isotope‐coded Derivatization Reagents For Lc–ms Analysismentioning

confidence: 82%

Section: Hydrazine Reagentsmentioning

confidence: 99%

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

El-Maghrabey

Kishikawa

Kuroda

2020

Biomedical Chromatography

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“…In total, 156 candidate carbonyl compounds are detected in human serum, of which 12 are further identified by synthetic standards. Using a targeted MRM mode, 44 carbonyls are found to be statistically different in myelogenous leukemia patients compared to healthy controls [223].…”

Section: Bioanalytical and Mass Spectrometric Methods For Charactementioning

confidence: 99%

Bioanalytical and Mass Spectrometric Methods for Aldehyde Profiling in Biological Fluids

Dator

Solivio

Villalta

et al. 2019

Toxics

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Human exposure to aldehydes is implicated in multiple diseases including diabetes, cardiovascular diseases, neurodegenerative disorders (i.e., Alzheimer’s and Parkinson’s Diseases), and cancer. Because these compounds are strong electrophiles, they can react with nucleophilic sites in DNA and proteins to form reversible and irreversible modifications. These modifications, if not eliminated or repaired, can lead to alteration in cellular homeostasis, cell death and ultimately contribute to disease pathogenesis. This review provides an overview of the current knowledge of the methods and applications of aldehyde exposure measurements, with a particular focus on bioanalytical and mass spectrometric techniques, including recent advances in mass spectrometry (MS)-based profiling methods for identifying potential biomarkers of aldehyde exposure. We discuss the various derivatization reagents used to capture small polar aldehydes and methods to quantify these compounds in biological matrices. In addition, we present emerging mass spectrometry-based methods, which use high-resolution accurate mass (HR/AM) analysis for characterizing carbonyl compounds and their potential applications in molecular epidemiology studies. With the availability of diverse bioanalytical methods presented here including simple and rapid techniques allowing remote monitoring of aldehydes, real-time imaging of aldehydic load in cells, advances in MS instrumentation, high performance chromatographic separation, and improved bioinformatics tools, the data acquired enable increased sensitivity for identifying specific aldehydes and new biomarkers of aldehyde exposure. Finally, the combination of these techniques with exciting new methods for single cell analysis provides the potential for detection and profiling of aldehydes at a cellular level, opening up the opportunity to minutely dissect their roles and biological consequences in cellular metabolism and diseases pathogenesis.

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“…QC samples (500 mL) were analyzed at 0, 4,8,12,16,20,24 hours to test their stability within 24 hours. The relative standard deviation (RSD) value was calculated.…”

Section: Methodology Developmentmentioning

confidence: 99%

“…Even for the same analyte, the ionization efficiency sometimes varies between two single runs. 16,17 Stable isotopic derivatization is an alternative to introducing stable isotopes to the analyte, which can be used as a substitute for the internal standard. Before analysis, an analogue of the analyte was marked with stable isotopes, and were added as the internal standard to improve the accuracy by minimizing the matrix effect and ionization difference, so that the absolute/relative quantication will be more accurate when compared with the traditional standard curve method.…”

mentioning

confidence: 99%

A sensitive approach for simultaneous quantification of carbonyl and hydroxyl steroids using 96-well SPE plates based on stable isotope coded-derivatization-UPLC-MRM: method development and application

et al. 2018

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Steroid hormones are crucial substances that mediate a wide range of vital physiological functions. Because of the important biological significance of steroids, this paper presents a new targeted metabolic method based on adding stable isotope tags to hydroxyl containing and carbonyl containing steroid hormones with two pairs of synthesized derivatization reagents: deuterium 4-(dimethylamino)-benzoic acid (D 4 -DMBA), and D 5 -Girard P (D 5 -GP) using of ultra performance liquid chromatography-multiple reaction monitoring (UPLC-MRM). Firstly, an Oasis PRiME hydrophilic-lipophilic balance (HLB) 96-well solid phase extraction plate was used to pretreat a number of biological samples simultaneously. Secondly, hydroxyl and carbonyl steroids were labeled using two pairs of synthetic reagents, namely DMBA and D 4 -DMBA, and GP and D 5 -GP, respectively. Thirdly, the mixed products were detected using UPLC-MRM and the mass spectroscopy conditions were optimized. Methodology development showed that the sensitivity was enhanced 1 to >500-fold. Finally, the new method was applied to analysis of urine samples of healthy males, females and rats. The results revealed that the method can be sensitive and reliable for simultaneous quantification of steroid hormones containing hydroxyl and carbonyl groups in 12 min in a single run. This method provided a powerful tool for studying the metabolic mechanism of steroids and contributed to the development of targeted metabolomics.

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Profiling of carbonyl compounds in serum by stable isotope labeling - Double precursor ion scan - Mass spectrometry analysis

Cited by 46 publications

References 33 publications

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

Current trends in isotope‐coded derivatization liquid chromatographic‐mass spectrometric analyses with special emphasis on their biomedical application

Bioanalytical and Mass Spectrometric Methods for Aldehyde Profiling in Biological Fluids

A sensitive approach for simultaneous quantification of carbonyl and hydroxyl steroids using 96-well SPE plates based on stable isotope coded-derivatization-UPLC-MRM: method development and application

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