Profiling of 2‐aminoacridone derivatised glycans by electrospray ionization mass spectrometry

Charlwood, Joanne; Langridge, J.; Tolson, David; Birrell, Helen; Camilleri, Patrick

doi:10.1002/(sici)1097-0231(19990130)13:2<107::aid-rcm463>3.3.co;2-g

“…The fragmentation patterns of three different types of N-glycans, high-mannose, complex and hybrid, derivatised with 2-aminoacridone were reported by Charlwood et al [297]. Relatively simple MS/MS fragmentation patterns have been observed for each type of glycan.…”

Section: Esi-msmentioning

confidence: 75%

Glycomics and Mass Spectrometry

Morelle¹,

Michalski

²

2005

CPD

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Proteomics is closely associated with the modifications of the gene product such as the post-translational events that yield functionally active gene products. Among these, glycosylation represents a critically important post-translational modification and is a target for proteomic research. Glycan moieties are involved in a wide variety of intracellular, cell-cell and cell-matrix recognition events. This is why understanding how glycosylation affects the activities and functions of proteins in health and disease represents a major challenge. The study of the glycome--the whole set of glycans produced in a single organism--is therefore essential to determine the functions of all genes. Mass spectrometry, in combination with modern separation methodologies, is one of the most powerful and versatile techniques for the structural analysis of oligosaccharides. This review provides a summary of the current knowledge for the mass spectrometric analysis of glycoproteins and their glycan structures.

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“…2-aminopyridine (2-AP) is generally preferred by investigators in Japan but in our hands, the product gives a weaker signal than 2-AB-derivatized glycans following ionization by both MALDI and ESI. 2-aminoacridone (2-AMAC) has been introduced by Camillari et al [67][68][69][70] for ESI studies and we have shown it to give very strong signals under MALDI conditions. Earlier work using FAB ionization included the preparation of a series of alkyl esters of 4-aminobenzoic acid [71]; these compounds also give strong ESI and MALDI signals.…”

Section: Reducing-terminal Derivatizationmentioning

confidence: 92%

“…gen as one of the adducts [67][68][69][70]. The presence of hydrogen tends to produce only glycosidic cleavage ions in the CID spectra.…”

Section: Fragmentation Produced By Collision-induced Dissociation (Cid)mentioning

confidence: 99%

Identification of protein-bound carbohydrates by mass spectrometry

Harvey¹

2001

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This review outlines the use of modern methods of mass spectrometry, mainly based on electrospray ionization and matrix-assisted lased desorption/ionization, for the identification of protein-bound carbohydrates. The techniques are briefly described together with methods for glycan release and purification prior to mass spectrometry. Fragmentation of glycans, produced under various conditions, is described in the context of obtaining structural information at the sensitivity required for proteomic work.

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“…2-aminopyridine (2-AP) is generally preferred by investigators in Japan but in our hands, the product gives a weaker signal than 2-AB-derivatized glycans following ionization by both MALDI and ESI. 2-aminoacridone (2-AMAC) has been introduced by Camillari et al [67][68][69][70] for ESI studies and we have shown it to give very strong signals under MALDI conditions. Earlier work using FAB ionization included the preparation of a series of alkyl esters of 4-aminobenzoic acid [71]; these compounds also give strong ESI and MALDI signals.…”

Section: Reducing-terminal Derivatizationmentioning

confidence: 92%

Identification of protein-bound carbohydrates by mass spectrometry

Harvey¹

2001

View full text Add to dashboard Cite

This review outlines the use of modern methods of mass spectrometry, mainly based on electrospray ionization and matrix-assisted lased desorption/ionization, for the identification of protein-bound carbohydrates. The techniques are briefly described together with methods for glycan release and purification prior to mass spectrometry. Fragmentation of glycans, produced under various conditions, is described in the context of obtaining structural information at the sensitivity required for proteomic work.

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Profiling of 2‐aminoacridone derivatised glycans by electrospray ionization mass spectrometry

Cited by 13 publications

References 7 publications

Glycomics and Mass Spectrometry

Glycomics and Mass Spectrometry

Identification of protein-bound carbohydrates by mass spectrometry

Identification of protein-bound carbohydrates by mass spectrometry

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