Profiling MicroRNA Expression in Hepatocellular Carcinoma Reveals MicroRNA-224 Up-regulation and Apoptosis Inhibitor-5 as a MicroRNA-224-specific Target

Wang, Yu; Lee, Alvin T.C.; Joel, Z.; Wang, Jingbo; Ren, Jianwei; Yang, Yuchen; Tantoso, Erwin; Li, Kuo Bin; Ooi, London Lucien; Tan, Patrick; Lee, Caroline

doi:10.1074/jbc.m707629200

Cited by 344 publications

(257 citation statements)

References 53 publications

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“…The global miRNA expression analyses (hierarchical clustering and principal component analysis) showed that the expression profiles of the miRNAs differed between the cisplatin-sensitive and -resistant cell lines (Figure 2), and expression levels of 45 miRNAs were changed by more than fourfold in cisplatin-sensitive cell lines as compared with cisplatin-resistant cell lines (Supplementary Table 1). Subsequently, the expression levels of the 10 miRNAs that were selected according to the miRNA microarray data and literature search [23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38][39] were validated by quantitative reverse transcription-PCR. This confirmed that miR-141, miR-21, miR-19b, miR-200a, miR-19a, miR-27a, miR-20a and miR-20b were expressed at significantly higher levels in the cisplatinresistant lines, and miR-205 and miR-224 at significantly lower levels than in the cisplatin-sensitive cell lines (Po0.05) (Table 1).…”

Section: Cisplatin-sensitive and -Resistant Human Escc Cell Linesmentioning

confidence: 99%

MicroRNA-141 confers resistance to cisplatin-induced apoptosis by targeting YAP1 in human esophageal squamous cell carcinoma

et al. 2011

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MicroRNAs (miRNAs) are endogenous non-coding RNAs that function as negative regulators of gene expression. Alterations in miRNA expression have been shown to affect tumor growth and response to chemotherapy. In this study, we explored the possible role of miRNAs in cisplatin resistance in esophageal squamous cell carcinoma (ESCC). First we assessed the sensitivity of nine human ESCC cell lines (KYSE series) to cisplatin using an in vitro cell viability assay, and then we compared the miRNA profiles of the cisplatin-sensitive and -resistant cell lines by miRNA microarray analysis. The two groups showed markedly different miRNA expression profiles, and 10 miRNAs were found to be regulated differentially between the two groups. When miR-141, which was the most highly expressed miRNA in the cisplatin-resistant cell lines, was expressed ectopically in the cisplatin-sensitive cell lines, cell viability after cisplatin treatment was increased significantly. Furthermore, we found that miR-141 directly targeted the 3¢-untranslated region of YAP1, which is known to have a crucial role in apoptosis induced by DNA-damaging agents, and thus downregulated YAP1 expression. Our study highlights an important regulatory role for miR-141 in the development of cisplatin resistance in ESCC.

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Section: Cisplatin-sensitive and -Resistant Human Escc Cell Linesmentioning

confidence: 99%

MicroRNA-141 confers resistance to cisplatin-induced apoptosis by targeting YAP1 in human esophageal squamous cell carcinoma

et al. 2011

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“…A closer look at the most enriched sites showed that these miRNAs have been previously observed to have altered expression in cancers (e.g., miR-124 in glioblastoma multiforme [Silber et al 2008]; miR-181 and miR-15/16 in B-cell chronic lymphocytic leukemia [Calin et al 2002;Pekarsky et al 2006]; miR-224 in thyroid tumors and in hepatocellular carcinoma [Nikiforova et al 2008;Wang et al 2008a]). …”

Section: Transcript Isoforms In Cancer Librariesmentioning

confidence: 99%

Next-generation tag sequencing for cancer gene expression profiling

Morrissy¹,

Morin²,

Delaney³

et al. 2009

Genome Res.

295

259

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We describe a new method, Tag-seq, which employs ultra high-throughput sequencing of 21 base pair cDNA tags for sensitive and cost-effective gene expression profiling. We compared Tag-seq data to LongSAGE data and observed improved representation of several classes of rare transcripts, including transcription factors, antisense transcripts, and intronic sequences, the latter possibly representing novel exons or genes. We observed increases in the diversity, abundance, and dynamic range of such rare transcripts and took advantage of the greater dynamic range of expression to identify, in cancers and normal libraries, altered expression ratios of alternative transcript isoforms. The strand-specific information of Tag-seq reads further allowed us to detect altered expression ratios of sense and antisense (S-AS) transcripts between cancer and normal libraries. S-AS transcripts were enriched in known cancer genes, while transcript isoforms were enriched in miRNA targeting sites. We found that transcript abundance had a stronger GC-bias in LongSAGE than Tagseq, such that AT-rich tags were less abundant than GC-rich tags in LongSAGE. Tag-seq also performed better in gene discovery, identifying >98% of genes detected by LongSAGE and profiling a distinct subset of the transcriptome characterized by AT-rich genes, which was expressed at levels below those detectable by LongSAGE. Overall, Tag-seq is sensitive to rare transcripts, has less sequence composition bias relative to LongSAGE, and allows differential expression analysis for a greater range of transcripts, including transcripts encoding important regulatory molecules.

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“…Depuis 2005, une dizaine d'études globales de l'expression des miARN dans les CHC a été publiée (Tableau 1) [19][20][21][22][23][24][25][26][27][28]. Varnholt et al ont montré dans une méta-analyse récente que les résultats de ces diffé-rentes études étaient assez peu concordants [29].…”

Section: Altérations De L'expression Des Miarn Dans Les Chcunclassified

“…Un des cas les plus intéressants est celui de miR-122 [30]. Ce miARN représente à lui seul 70 % de l'expression des miARN du foie ; essentiel [19][20][21][22][23][24][25][26][27][28]. Les miARN dont l'expression est altérée de façon similaire dans au moins 2 études ou plus sont soulignés.…”

Section: Faible Expression De Plusieurs Miarn Dans Les Chcunclassified

Micro-ARN (miARN) et cancer : le cas des tumeurs hépatocellulaires

Ladeiro

Zucman‐Rossi

2009

Med Sci (Paris)

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Profiling MicroRNA Expression in Hepatocellular Carcinoma Reveals MicroRNA-224 Up-regulation and Apoptosis Inhibitor-5 as a MicroRNA-224-specific Target

Cited by 344 publications

References 53 publications

MicroRNA-141 confers resistance to cisplatin-induced apoptosis by targeting YAP1 in human esophageal squamous cell carcinoma

MicroRNA-141 confers resistance to cisplatin-induced apoptosis by targeting YAP1 in human esophageal squamous cell carcinoma

Next-generation tag sequencing for cancer gene expression profiling

Micro-ARN (miARN) et cancer : le cas des tumeurs hépatocellulaires

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