Profiling Methods to Identify Cold-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry

Dethloff, Frederik; Erban, Alexander; Orf, Isabel; Alpers, Jessica; Fehrle, Ines; Beine-Golovchuk, Olga; Schmidt, Stefanie; Schwachtje, Jens; Kopka, Joachim

doi:10.1007/978-1-4939-0844-8_14

Cited by 43 publications

(45 citation statements)

References 26 publications

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“…Fifty milligrams were used for extraction of primary metabolites as described by Dethloff et al . (). The number of biological replicates is given in Supporting Information Table S1.…”

Section: Methodsmentioning

confidence: 97%

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The drought response of potato reference cultivars with contrasting tolerance

Sprenger

Kurowsky

Horn

et al. 2016

Plant Cell & Environment

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Systems responses to drought stress of four potato reference cultivars with differential drought tolerance (Solanum tuberosum L.) were investigated by metabolome profiling and RNA sequencing. Systems analysis was based on independent field and greenhouse trials. Robust differential drought responses across all cultivars under both conditions comprised changes of proline, raffinose, galactinol, arabitol, arabinonic acid, chlorogenic acid and 102 transcript levels. The encoded genes contained a high proportion of heat shock proteins and proteins with signalling or regulatory functions, for example, a homolog of abscisic acid receptor PYL4. Constitutive differences of the tolerant compared with the sensitive cultivars included arbutin, octopamine, ribitol and 248 transcripts. The gene products of many of these transcripts were pathogen response related, such as receptor kinases, or regulatory proteins, for example, a homolog of the Arabidopsis FOUR LIPS MYB-regulator of stomatal cell proliferation. Functional enrichment analyses imply heat stress as a major acclimation component of potato leaves to long-term drought stress. Enhanced heat stress during drought can be caused by loss of transpiration cooling. This effect and CO limitation are the main consequences of drought-induced or abscisic acid-induced stomatal closure. Constitutive differences in metabolite and transcript levels between tolerant and sensitive cultivars indicate interactions of drought tolerance and pathogen resistance in potato.

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“…Fifty milligrams were used for extraction of primary metabolites as described by Dethloff et al . (). The number of biological replicates is given in Supporting Information Table S1.…”

Section: Methodsmentioning

confidence: 97%

“…Retention index standardization and chemical derivatization for GC analysis were performed as specified by Dethloff et al . (). Finally, 1 μ L of the sample was injected into a Varian FactorFour column (VF‐5 ms) of the GC unit.…”

Section: Methodsmentioning

confidence: 97%

The drought response of potato reference cultivars with contrasting tolerance

Sprenger

Kurowsky

Horn

et al. 2016

Plant Cell & Environment

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“…The mixture was further centrifuged at 5,000 g for 10 min and the supernatant was dried in a vacuum concentrator. Sample derivatization procedure was followed previously (Dethloff et al, 2014). The dried samples were derivatized at 30°C for 90 min with 40 μL of 2% (m/v) methoxyamine hydrochloride in pyridine.…”

Section: Methodsmentioning

confidence: 99%

Insights from the Cold Transcriptome and Metabolome of Dendrobium officinale: Global Reprogramming of Metabolic and Gene Regulation Networks during Cold Acclimation

Chen

et al. 2016

Front. Plant Sci.

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Plant cold acclimation (CA) is a genetically complex phenomenon involving gene regulation and expression. Little is known about the cascading pattern of gene regulatroy network and the link between genes and metabolites during CA. Dendrobium officinale (DOKM) is an important medicinal and ornamental plant and hypersensitive to low temperature. Here, we used the large scale metabolomic and transcriptomic technologies to reveal the response to CA in DOKM seedlings based on the physiological profile analyses. Lowering temperature from 4 to –2°C resulted in significant increase (P < 0.01) in antioxidant activities and electrolyte leakage (EL) during 24 h. The fitness CA piont of 0°C and control (20°C) during 20 h were firstly obtained according to physiological analyses. Subsequently, massive transcriptome and metabolome reprogramming occurred during CA. The gene to metabolite network demonstrated that the CA associated processes are highly energy demanding through activating hydrolysis of sugars, amino acids catabolism and citrate cycle. The expression levels of 2,767 genes were significantly affected by CA, including 153-fold upregulation of CBF transcription factor, 56-fold upregulation of MAPKKK16 protein kinase. Moreover, the gene interaction and regulation network analysis revealed that the CA as an active process, was regulated at the transcriptional, post-transcriptional, translational and post-translational levels. Our findings highligted a comprehensive regulatory mechanism including cold signal transduction, transcriptional regulation, and gene expression, which contributes a deeper understanding of the highly complex regulatory program during CA in DOKM. Some marker genes identified in DOKM seedlings will allow us to understand the role of each individual during CA by further functional analyses.

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“…Precursors or conjugated forms of signalling factors such as phytohormones or certain plant metabolites may accumulate in response to a priming stimulus (Bruce et al, 2007;Kaplan et al, 2007;Galis et al, 2009;Dervinis et al, 2010;Pastor et al, 2013;Schulz, Herde & Romeis, 2013;Zeier, 2013;Dethloff et al, 2014). Precursors or conjugated forms of signalling factors such as phytohormones or certain plant metabolites may accumulate in response to a priming stimulus (Bruce et al, 2007;Kaplan et al, 2007;Galis et al, 2009;Dervinis et al, 2010;Pastor et al, 2013;Schulz, Herde & Romeis, 2013;Zeier, 2013;Dethloff et al, 2014).…”

Section: Molecular Mechanisms Of Priming and Memorymentioning

confidence: 99%

Priming and memory of stress responses in organisms lacking a nervous system

et al. 2015

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Experience and memory of environmental stimuli that indicate future stress can prepare (prime) organismic stress responses even in species lacking a nervous system. The process through which such organisms prepare their phenotype for an improved response to future stress has been termed 'priming'. However, other terms are also used for this phenomenon, especially when considering priming in different types of organisms and when referring to different stressors. Here we propose a conceptual framework for priming of stress responses in bacteria, fungi and plants which allows comparison of priming with other terms, e.g. adaptation, acclimation, induction, acquired resistance and cross protection. We address spatial and temporal aspects of priming and highlight current knowledge about the mechanisms necessary for information storage which range from epigenetic marks to the accumulation of (dormant) signalling molecules. Furthermore, we outline possible patterns of primed stress responses. Finally, we link the ability of organisms to become primed for stress responses (their 'primability') with evolutionary ecology aspects and discuss which properties of an organism and its environment may favour the evolution of priming of stress responses.

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Profiling Methods to Identify Cold-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry

Cited by 43 publications

References 26 publications

The drought response of potato reference cultivars with contrasting tolerance

The drought response of potato reference cultivars with contrasting tolerance

Insights from the Cold Transcriptome and Metabolome of Dendrobium officinale: Global Reprogramming of Metabolic and Gene Regulation Networks during Cold Acclimation

Priming and memory of stress responses in organisms lacking a nervous system

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