Profiling Metal Oxides with Lipids: Magnetic Liposomal Nanoparticles Displaying DNA and Proteins

Wang, Feng; Zhang, Xiaohan; Liu, Yibo; Lin, Ziliang; Liu, Biwu; Liu, Juewen

doi:10.1002/anie.201606603

Cited by 49 publications

(70 citation statements)

References 47 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is the first time that we observed DOPC liposome leakage when mixed with a non-silica and non-cationic oxide. 46 At pH 7.6, CeO2 is near charge neutral (slightly negatively charged), and thus the leakage is unlikely due to membrane damage by cationic nanomaterials. 46,47 From the surface chemistry standpoint, CeO2 is more similar to TiO2 in terms of containing a hard Lewis acid metal favoring strong phosphate interaction, which is demonstrated in this work.…”

mentioning

confidence: 99%

“…46 At pH 7.6, CeO2 is near charge neutral (slightly negatively charged), and thus the leakage is unlikely due to membrane damage by cationic nanomaterials. 46,47 From the surface chemistry standpoint, CeO2 is more similar to TiO2 in terms of containing a hard Lewis acid metal favoring strong phosphate interaction, which is demonstrated in this work. Therefore, we want to understand whether this is due to fully ruptured liposomes or local membrane damages.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Adsorption of Nanoceria by Phosphocholine Liposomes

Liu

2016

Langmuir

Self Cite

View full text Add to dashboard Cite

Nanoceria (CeO2 nanoparticle) possesses a number of enzyme-like activities. In particular, it scavenges reactive oxygen species (ROS) leading in vitro and in vivo anti-oxidation studies. An important aspect of fundamental physical understanding is its interaction with lipid membranes, the man component of the cell membrane. In this work, adsorption of nanoceria onto phosphocholine (PC) liposomes was performed. PC lipids are the main constituent of the cell outer membrane. Using fluorescence quenching assay, nanoceria adsorption isotherm was determined at various pH's and ionic strengths. A non-Langmuir isotherm occurred at pH 4.0 due to lateral electrostatic repulsion among adsorbed cationic nanoceria. The phosphate group in the PC lipid is mainly responsible for the interaction, and adsorbed nanoceria can be displaced by free inorganic phosphate. The tendency of the system to form large aggregates is a function of pH and the concentration of nanoceria, attributable to nanoceria being positively charged at pH 4 and neural at physiological pH. Calcein leakage test indicates that nanoceria induces liposome leakage due to transient lipid phase transition, and cryo-TEM indicates that the overall shape of the liposome is retained although deformation is still observed. This study provides fundamental biointerfacial information at a molecular level regarding the interaction of nanoceria and model cell membranes.3

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Adsorption of Nanoceria by Phosphocholine Liposomes

Liu

2016

Langmuir

Self Cite

View full text Add to dashboard Cite

show abstract

“…Only when all the adenines are replaced by thymines, some difference can be detected . DNA can also use its phosphate backbone to bind and representative examples are metal oxides . In such cases, the effect of DNA base sequence is even smaller.…”

Section: Inorganic Surfaces Versus Molecules As An Aptamer Targetmentioning

confidence: 99%

“…While the affinity for different bases are quite different, each base can be adsorbed very strongly approaching chemical adsorption region in vacuum . b) Metal oxide surfaces bind to DNA via its phosphate backbone, and the effect of DNA base is relatively small . Free inorganic phosphate ions can displace adsorbed DNA from metal oxides in most cases.…”

Section: Classification Of Surfaces For Dna Bindingmentioning

confidence: 99%

See 1 more Smart Citation

Selection and Screening of DNA Aptamers for Inorganic Nanomaterials

Zhou

Huang

Yang

et al. 2017

Chemistry A European J

Self Cite

View full text Add to dashboard Cite

Searching for DNA sequences that can strongly and selectively bind to inorganic surfaces is a long-standing topic in bionanotechnology, analytical chemistry and biointerface research. This can be achieved either by aptamer selection starting with a very large library of ≈10 random DNA sequences, or by careful screening of a much smaller library (usually from a few to a few hundred) with rationally designed sequences. Unlike typical molecular targets, inorganic surfaces often have quite strong DNA adsorption affinities due to polyvalent binding and even chemical interactions. This leads to a very high background binding making aptamer selection difficult. Screening, on the other hand, can be designed to compare relative binding affinities of different DNA sequences and could be more appropriate for inorganic surfaces. The resulting sequences have been used for DNA-directed assembly, sorting of carbon nanotubes, and DNA-controlled growth of inorganic nanomaterials. It was recently discovered that poly-cytosine (C) DNA can strongly bind to a diverse range of nanomaterials including nanocarbons (graphene oxide and carbon nanotubes), various metal oxides and transition-metal dichalcogenides. In this Concept article, we articulate the need for screening and potential artifacts associated with traditional aptamer selection methods for inorganic surfaces. Representative examples of application are discussed, and a few future research opportunities are proposed towards the end of this article.

show abstract

Nanotechnology Strategies for Plant Genetic Engineering

Yan

Zhu

et al. 2022

Advanced Materials

Self Cite

View full text Add to dashboard Cite

Plant genetic engineering is essential for improving crop yield, quality, and resistance to abiotic/biotic stresses for sustainable agriculture. Agrobacterium‐, biolistic bombardment‐, electroporation‐, and poly(ethylene glycol) (PEG)‐mediated genetic‐transformation systems are extensively used in plant genetic engineering. However, these systems have limitations, including species dependency, destruction of plant tissues, low transformation efficiency, and high cost. Recently, nanotechnology‐based gene‐delivery methods have been developed for plant genetic transformation. This nanostrategy shows excellent transformation efficiency, good biocompatibility, adequate protection of exogenous nucleic acids, and the potential for plant regeneration. However, the nanomaterial‐mediated gene‐delivery system in plants is still in its infancy, and there are many challenges for its broad applications. Herein, the conventional genetic transformation techniques used in plants are briefly discussed. After that, the progress in the development of nanomaterial‐based gene‐delivery systems is considered. CRISPR‐Cas‐mediated genome editing and its combined applications with plant nanotechnology are also discussed. The conceptual innovations, methods, and practical applications of nanomaterial‐mediated genetic transformation summarized herein will be beneficial for promoting plant genetic engineering in modern agriculture.

show abstract

Profiling Metal Oxides with Lipids: Magnetic Liposomal Nanoparticles Displaying DNA and Proteins

Cited by 49 publications

References 47 publications

Adsorption of Nanoceria by Phosphocholine Liposomes

Adsorption of Nanoceria by Phosphocholine Liposomes

Selection and Screening of DNA Aptamers for Inorganic Nanomaterials

Nanotechnology Strategies for Plant Genetic Engineering

Contact Info

Product

Resources

About