Profiling lncRNA alterations during TNF‑α induced osteogenic differentiation of dental pulp stem cells

Abstract

The multipotent and easily accessible characteristics of dental pulp stem cells (DPSCs) make them a promising target for bone tissue engineering. Long non-coding RNAs (lncRNAs) have an important role in the osteogenic differentiation of mesenchymal stem cells. Nevertheless, whether lncRNAs are involved in the osteogenic differentiation of DPSCs remains unclear. The present study examined the expression alterations of lncRNAs in tumor necrosis factor-α induced osteogenic differentiation of DPSCs. Following identification of differentially expressed lncRNAs at different time points by reverse transcription-quantitative polymerase chain reaction, profiling analysis was performed and a profile was further validated, in which lncRNA expression levels demonstrated significant upregulation. The next generation sequencing analysis identified 77 (58 upregulated and 19 downregulated) and 133 differentially expressed lncRNAs (73 upregulated and 60 downregulated) at 7 and 14 days post-treatment, respectively. In addition, 34 lncRNAs were predicted to be strongly associated with 336 mRNA transcripts that underwent significant alterations during osteogenic differentiation. The present data demonstrated that one lncRNA, X inactive specific transcript, is essential for efficient osteogenic differentiation of DPSCs by alkaline phosphatase staining. In summary, the present findings provide insight for the understanding of how non-coding RNAs are involved in regulating the osteogenic differentiation of DPSCs, which may further advance the translational studies of bone tissue engineering.

Epigenetic Regulation of Dental Pulp Stem Cell Fate

“…Studies have confirmed that lncRNAs are also associated with the osteogenic differentiation of DPSCs. Examination of the expression of lncRNAs during TNF- α induced osteogenic differentiation revealed 58 upregulated and 19 downregulated lncRNAs on day 7 and 73 upregulated and 60 downregulated lncRNAs on day 14 [ 181 ]. lncRNA colon cancer-associated transcript 1 (CCAT1) is initially discovered to participate in metabolic, migratory, and proliferative processes in some cancers [ 182 ].…”

Mesenchymal Stem Cell-Derived from Dental Tissues-Related lncRNAs: A New Regulator in Osteogenic Differentiation

“…89 lncRNAs were diferentially expressed, 28 upregulated and 61 down-regulated [60]. In another study, osteogenic diferentiation of DPSCs after TNF-α induction, diferentially expressed lncRNAs were detected by RT-PCR after 7 days, and there were 77 (58 increased and 19 decreased) and 133 (73 increased and 60 decreased) diferentially expressed lncRNAs, respectively after 14 days of treatment [61]. Te osteogenesis mechanism of DPSCs regulated by lncRNA can be seen in Figure 3.…”

Epigenetic regulation of dental pulp stem cells and its potential in regenerative endodontics

“…lncRNAs undergo transitional alterations during TNF-α-mediated osteogenic differentiation of pDPSCs, since lncRNA expression patterns differ after 7 and 14 d of treatment with TNF-α. These alterations in lncRNAs expression are predicted to be associated with mRNA alterations at day 7 and 14 posts TNF-α induction[ 97 ]. lncRNA DANCR declines with time during odontoblast-like differentiation of pDPSCs.…”