1998
DOI: 10.1006/geno.1998.5354
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Profiling Expression Patterns and Isolating Differentially Expressed Genes by cDNA Microarray System with Colorimetry Detection

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Cited by 214 publications
(138 citation statements)
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“…It is important at this point to keep in mind that published expression pro®ling work with microarrays or oligonucleotide chips uses microgram amounts of poly(A) + RNA for probe preparation (Schena et al, 1996;Lockhart et al, 1996), or resorts to probe ampli®cation methods that may skew relative abundance values. Further miniaturization using Nylon microarrays (Chen et al, 1998) and radioactive probes (with a very high-resolution detection system) can bring down sample requirements by a large factor (Bertucci et al, in preparation), but in the implementation described here, the method is already able to provide signi®cant information in clinically relevant situations.…”
Section: Resultsmentioning
confidence: 99%
See 1 more Smart Citation
“…It is important at this point to keep in mind that published expression pro®ling work with microarrays or oligonucleotide chips uses microgram amounts of poly(A) + RNA for probe preparation (Schena et al, 1996;Lockhart et al, 1996), or resorts to probe ampli®cation methods that may skew relative abundance values. Further miniaturization using Nylon microarrays (Chen et al, 1998) and radioactive probes (with a very high-resolution detection system) can bring down sample requirements by a large factor (Bertucci et al, in preparation), but in the implementation described here, the method is already able to provide signi®cant information in clinically relevant situations.…”
Section: Resultsmentioning
confidence: 99%
“…The most productive implementation uses hybridization of complex probes prepared from cell or tissue RNA to`macroarrays' of cDNA inserts on Nylon membranes (Bernard et al, 1996;Tsou et al, 1998), miniaturized`microarrays' constructed on glass slides (Schena et al, 1995;DeRisi et al, 1996;Shalon et al, 1996) or on Nylon membranes (Chen et al, 1998), or`DNA chips' carrying oligonucleotides (Lockhart et al, 1996;Wodicka et al, 1997). In all cases, hybridization intensity can be used to derive expression data for each gene represented on the array.…”
mentioning
confidence: 99%
“…2-4,6 -8,22 In addition, transcriptional silencing by methylation of CpG islands in the 5Ј promoter region of the caveolin-1 gene was once found in two human breast cancer cell lines that failed to express the caveolin-1 protein. 23 Our previous microarray data from the comparison of genes differentially expressed in cell lines with varying invasive capability suggested a possible reciprocal transcriptional regulation between caveolin-1 and HEK2, TNF-R, or protein kinase C during lung cell transformation, 20 although further experimentation is needed to confirm this guess.…”
Section: Discussionmentioning
confidence: 99%
“…20 Briefly, mRNA was extracted from each CL cell line and reverse-transcribed into cDNA labeled with biotin. The biotin-labeled cDNA was used as probes and hybridized with the membranes that contained 9600 nonredundant expressed sequence tag clones selected from human cDNA libraries.…”
Section: Microarray Analysismentioning
confidence: 99%
“…Gene activity was measured by a cDNA microarray method with enzyme colorimetry detection (13). The mRNAs of the pulmonary arteries and veins were amplified by using an in vitro transcription method (14) before they were labeled with biotin during the reverse transcription process.…”
Section: Methodsmentioning
confidence: 99%