Profiling DNA Methylation Differences Between Inbred Mouse Strains on the Illumina Human Infinium MethylationEPIC Microarray

Gujar, Hemant; Liang, Jane W.; Wong, Nicholas C.; Mozhui, Khyobeni

doi:10.1101/194464

Cited by 3 publications

(3 citation statements)

References 58 publications

(32 reference statements)

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“…This is still a relatively modest number of age‐dependent CpGs, and this is likely due to the small sample size and the genotype heterogeneity of the present cohort. Another contributing factor may be the methylome assay we used, as MBD‐seq provides lower quantitative sensitivity (Gujar, Liang, Wong, & Mozhui, 2018). Nonetheless, MBD‐sequencing still delivers highly sensitive and replicable quantification of genome‐wide methylation (Aberg et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Body weight and high‐fat diet are associated with epigenetic aging in female members of the BXD murine family

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Body weight and high‐fat diet are associated with epigenetic aging in female members of the BXD murine family

et al. 2020

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“…The recently released Illumina MethylationEPIC BeadChip has a higher coverage (850 000 CpG sites) than the HumanMethylation450K and high reproducibility, adding a potentially efficient platform to the currently available techniques . The chip has been used and evaluated in a number of recent studies …”

Section: Introductionmentioning

confidence: 99%

“…22 The chip has been used and evaluated in a number of recent studies. 23,24 Epigenome-wide association studies (EWAS) assess methylation of the DNA and its associations with gene expression, phenotypes or exposures. Although epigenetics as a general term also include histone modifications and other mechanisms that may alter gene expression, EWAS have been most widely used to study DNA methylation.…”

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Epigenome‐wide association studies in asthma: A systematic review

Edris

Dekker

Melén

et al. 2019

Clin Experimental Allergy

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Objective Asthma is a common chronic respiratory airway disease influenced by environmental factors and possibly their interaction with the human genome causing epigenetic changes. Epigenome‐wide association studies (EWAS) have mainly investigated DNA methylation and its association with disease or traits, exposure factors or gene expression. This systematic review aimed to identify all EWAS assessing differentially methylated sites associated with asthma in humans. Design Structured systematic literature search following PRISMA guidelines, Newcastle‐Ottawa Scale (NOS) for cohort studies was used for bias assessment. Data Sources We searched PubMed and Embase databases from 2005 to 2019. Eligibility Criteria Epigenome‐wide association studies testing association between differential methylation and asthma in humans. Results Overall, we identified 16 EWAS studies complying with our search criteria. Twelve studies were conducted on children, and 10 were conducted on sample sizes <150 subjects. Four hundred and nineteen CpGs were reported in children studies after correction for multiple testing. In the adult studies, thousands of differentially methylated sites were identified. Differential methylation in inflammatory‐related genes correlated with higher levels of gene expressions of inflammatory modulators in asthma. Differentially methylated genes associated with asthma included SMAD3, SERPINC1, PROK1, IL13, RUNX3 and TIGIT. Forty‐one CpGs were replicated at least once in blood samples, and 28 CpGs were replicated in nasal samples. Conclusion Although many differentially methylated CpGs in genes known to be involved in asthma have been identified in EWAS to date, we conclude that further studies of larger sample sizes and analyses of differential methylation between different phenotypes are needed in order to comprehensively evaluate the role of epigenetic factors in the pathophysiology and heterogeneity of asthma, and the potential clinical utility to predict or classify patients with asthma.

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Study Protocol for a Randomized Controlled Trial of Choral Singing Intervention to Prevent Cognitive Decline in At-Risk Older Adults Living in the Community

Tan

Tsakok²,

et al. 2018

Front. Aging Neurosci.

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Introduction: This study is a parallel-arm randomized controlled trial evaluating choral singing’s efficacy and underlying mechanisms in preventing cognitive decline in at-risk older participants.Methods: Three-hundred and sixty community-dwelling, non-demented older participants are recruited for a 2-year intervention. Inclusion criteria are self-reported cognitive complaints, early cognitive impairment based on neuropsychological test scores or multiple risk factors of dementia. Participants are randomized to either weekly choral singing sessions or general health education. The primary outcome is cognitive performance, measured by a composite cognitive test score (CCTS). Secondary outcomes include depression, anxiety and neuropsychiatric symptoms; perceived stress; sleep quality and severity of dementia symptoms. Underlying mechanisms are examined using blood- and urine-based biomarkers and neuroimaging.Results: Screening began in July 2016. The first group of participants (n = 93) have been recruited. Intervention and control treatments are ongoing and will end in December 2019.Discussion: An evidence-based singing intervention for dementia prevention holds potential for healthcare savings and societal welfare.Trial Registration: NCT02919748, IRB Approval Number: NUS 2508.

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Profiling DNA Methylation Differences Between Inbred Mouse Strains on the Illumina Human Infinium MethylationEPIC Microarray

Abstract: 9The Illumina Infinium MethylationEPIC provides an efficient platform for profiling DNA

Cited by 3 publications

References 58 publications

Body weight and high‐fat diet are associated with epigenetic aging in female members of the BXD murine family

Body weight and high‐fat diet are associated with epigenetic aging in female members of the BXD murine family

Epigenome‐wide association studies in asthma: A systematic review

Study Protocol for a Randomized Controlled Trial of Choral Singing Intervention to Prevent Cognitive Decline in At-Risk Older Adults Living in the Community

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