Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition

Zhang, Chun Xia; Wang, Meng; Li, Yisi; Zhang, Yi

doi:10.1126/sciadv.abj3967

“…S2, B and C). The variations between replicates were comparable to the Ribo-seq data in mouse oocytes and early embryos ( 18 , 21 ), as well as mESCs ( 22 ) (fig. S2D).…”

Section: Resultssupporting

confidence: 79%

Translatome and transcriptome co-profiling reveals a role of TPRXs in human zygotic genome activation

Zou

¹

,

Zhang

²

,

Wang

³

et al. 2022

Science

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Translational regulation plays a critical role during the oocyte-to-embryo transition (OET) and zygotic genome activation (ZGA). Here, we integrated ultra-low-input Ribo-seq with mRNA-seq to co-profile the translatome and transcriptome in human oocytes and early embryos. Comparison with mouse counterparts identified widespread differentially translated genes functioning in epigenetic reprogramming, transposon defense, and small RNA biogenesis, in part driven by species-specific regulatory elements in 3′ untranslated regions. Moreover, PRD-like homeobox transcription factors, including TPRXL, TPRX1, and TPRX2, are highly translated around ZGA. TPRX1/2/L knockdown leads to defective ZGA and preimplantation development. Ectopically expressed TPRXs bind and activate key ZGA genes in human embryonic stem cells. These data reveal the conservation and divergence of translation landscapes during OET and identify critical regulators of human ZGA.

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“…Our current results document a program for rapid resetting of the early embryonic epigenome that is controlled by carefully orchestrated translation of maternal mRNA. A recent profiling of translated maternal mRNAs in mouse zygotes supports our findings of the importance of selective translation of chromatin remodeling complexes for ZGA 56 . Although the necessity of translation to trigger ZGA and the start of embryogenesis was previously suggested 6-8 , its regulatory mechanisms have remained unclear, and it has not been the focus of investigations into the maternal-to-zygotic transition.…”

Section: Discussionsupporting

confidence: 86%

Germ-cell specific eIF4E1B regulates maternal RNA translation to ensure zygotic genome activation

Yang

¹

,

Xin

²

,

Feng

³

et al. 2022

Preprint

3

0

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Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remains unknown. Using gene-edited mice, we document that the eukaryotic translation initiation factor 4E family member 1B (eIF4E1B) is the regulator of maternal mRNA translation that ensures subsequent reprogramming of the zygotic genome. In oocytes, the germ-cell specific eIF4E1B binds to mRNAs encoding chromatin remodeling complexes as well as reprogramming factors to protect them from degradation and promote their translation in zygotes. These protein products establish an open chromatin landscape in one-cell zygotes and enable transcription. Our results define a program for rapid resetting of the zygotic epigenome that is regulated by maternal mRNA translation and provides new insight into the mammalian maternal-to-zygotic transition.

show abstract

“…Our current results document a program for rapid resetting of the early embryonic epigenome that is controlled by carefully orchestrated translation of maternal mRNA. A recent profiling of translated maternal mRNAs in mouse zygotes supports our findings of the importance of selective translation of chromatin remodeling complexes for ZGA 56 .…”

Section: Discussionsupporting

confidence: 86%

Germ-cell specific eIF4E1B regulates maternal RNA translation to ensure zygotic genome activation

Yang

¹

,

Xin

²

,

Feng

³

et al. 2022

Preprint

2

0

View full text Add to dashboard Cite

Translation of maternal mRNAs is detected before transcription of zygotic genes and is essential for mammalian embryo development. How certain maternal mRNAs are selected for translation instead of degradation and how this burst of translation affects zygotic genome activation remains unknown. Using gene-edited mice, we document that the eukaryotic translation initiation factor 4E family member 1B (eIF4E1B) is the regulator of maternal mRNA translation that ensures subsequent reprogramming of the zygotic genome. In oocytes, the germ-cell specific eIF4E1B binds to mRNAs encoding chromatin remodeling complexes as well as reprogramming factors to protect them from degradation and promote their translation in zygotes. These protein products establish an open chromatin landscape in one-cell zygotes and enable transcription. Our results define a program for rapid resetting of the zygotic epigenome that is regulated by maternal mRNA translation and provides new insight into the mammalian maternal-to-zygotic transition.

show abstract

Profiling and functional characterization of maternal mRNA translation during mouse maternal-to-zygotic transition

Cited by 63 publications

References 73 publications

Translatome and transcriptome co-profiling reveals a role of TPRXs in human zygotic genome activation

Translatome and transcriptome co-profiling reveals a role of TPRXs in human zygotic genome activation

Germ-cell specific eIF4E1B regulates maternal RNA translation to ensure zygotic genome activation

Germ-cell specific eIF4E1B regulates maternal RNA translation to ensure zygotic genome activation

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