Profilin is associated with the plasma membrane in microspores and pollen

Witsch, Matthias von; Baluška, Frantǐsek; Staiger, Christopher J.; Volkmann, Dieter

doi:10.1016/s0171-9335(98)80089-7

Cited by 35 publications

(17 citation statements)

References 52 publications

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“…Plant profilins have been shown to rescue profilin mutants in Dictyostelium (Karakesisoglou et al, 1996) and yeast (Christensen et al, 1996), suggesting that they share many of these mechanistic capabilities with their counterparts in other kingdoms. Some of these properties have been characterized experimentally for a few plant profilins (Staiger et al, 1994; Gibbon et al, 1997 Gibbon et al, , 1998 Clarke et al, 1998;von Witsch et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Small Changes in the Regulation of One Arabidopsis Profilin Isovariant, PRF1, Alter Seedling Development

2001

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Profilin (PRF) is a low-molecular-weight actin binding protein encoded by a diverse gene family in plants. Arabidopsis PRF1 transcripts are moderately well expressed in all vegetative organs. A regulatory mutant in PRF1 , prf1-1 , was isolated from a library of T-DNA insertions. The insertion disrupted the promoter region of PRF1 100 bp upstream from the transcriptional start site. Although steady state levels of PRF1 transcripts appeared normal in mature prf1-1 plants, the levels in young seedlings were only one-half those observed in wild type. Reactions with a PRF1 isovariant-specific monoclonal antiserum and general anti-profilin antisera demonstrated that PRF1 protein levels also were one-half those found in wild-type seedlings, although total profilin levels were unaffected. Mutant seedlings no longer could downregulate PRF1 levels in the light, as did wild type. Consistent with their molecular phenotypes, young mutant seedlings displayed several morphological phenotypes but developed into apparently normal adult plants. Their initial germination rate and development were slow, and they produced excessive numbers of root hairs. Mutant seedlings had abnormally raised cotyledons, elongated hypocotyls, and elongated cells in the hypocotyl, typical of phenotypes associated with some defects in light and circadian responses. A wild-type PRF1 transgene fully complements the hypocotyl phenotypes in the prf1-1 mutant. The ability of profilin to regulate actin polymerization and participate directly in signal transduction pathways is discussed in light of the prf1-1 phenotypes. INTRODUCTIONThe actin cytoskeleton contributes to many of the dynamic processes directing plant development: cell polarity, division plane determination, cell elongation, and cell wall deposition (Meagher et al., 1999b). As seedlings emerge from the soil and respond to light, significant changes occur in plant morphology that must be directed at the cellular level by alterations in the cytoskeleton (Meagher et al., 2000). Because the actin binding protein profilin is particularly important to the dynamics of actin polymerization and sequestration, the regulation of plant profilin expression in cells and organs responding to changes in light levels should affect plant development. This article provides initial data demonstrating the connection between profilin regulation and plant morphology.Profilin binds monomeric G-actin and participates in several cytoskeletal functions (Gibbon and Staiger, 2000) with the potential to alter cellular and organismal development. Profilin accelerates assembly at the barbed ends of actin filaments and sequesters actin monomers, which decreases the pool of polymerizable actin once barbed ends are capped (Carlier et al., 1993;Pantaloni and Carlier, 1993;Perelroizen et al., 1996). Profilin activates actin for assembly by catalyzing ADP-to-ATP exchange on actin monomers. Profilin binds tightly to poly-L -proline, a motif present in some proteins involved in regulating the actin cytoskeleton (Ahern-Djamali et al....

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Section: Introductionmentioning

confidence: 99%

Small Changes in the Regulation of One Arabidopsis Profilin Isovariant, PRF1, Alter Seedling Development

2001

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“…Plant profilins are encoded by multigene families and have different isoforms. For example, Arabidopsis may contain at least 5 profilin isoforms [65], and maize profilin family has six members [49,159]. It has been well demonstrated that these isoforms have distinct patterns of expression in specific cell type, organ, tissue and developmental stage.…”

Section: Profilinmentioning

confidence: 99%

“…Many results show that profilin is distributed uniformly in vegetative or reproductive cell types of Arabidopsis [75], as well as in the pollen tubes of tobacco [75] and Lilium [155]. However, profilin is localized to the plasma membrane in microspores of birch pollen [159], and it is also detected close to the site of pollen tube emergence, pollen exine and the tip region of olive pollen tube [109]. In the root hair, another polargrowing cell type, profilin is specifically expressed in the apical region [7,14].…”

Section: Profilinmentioning

confidence: 99%

Actin-Binding Proteins and Actin Dynamics in Plant Cells

Huang¹,

Xiang²,

Ren

2010

Advances in Plant Biology

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“…This appears not to be the case for growing lily pollen tubes that were analyzed by fluorescent-analog cytochemistry and stateof-the-art fixation methods (Vidali and Hepler 1997). However, plasma-membrane-associated profilin has been observed in other cell types and/or other stages of pollen development (e.g., von Witsch et al 1998, Braun et al 1999, B.-Q. Huang pers.…”

Section: Profilin: a Complex Regulator Of Actin Polymerizationmentioning

confidence: 99%

Actin and actin-binding proteins in higher plants

2001

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The actin cytoskeleton is a complex and dynamic structure that participates in diverse cellular events which contribute to plant morphogenesis and development. Plant actins and associated actin-binding proteins are encoded by large, differentially expressed gene families. The complexity of these gene families is thought to have been conserved to maintain a pool of protein isovariants with unique properties, thus providing a mechanistic basis for the observed diversity of plant actin functions. Plants contain actin-binding proteins which regulate the supramolecular organization and function of the actin cytoskeleton, including monomer-binding proteins (profilin), severing and dynamizing proteins (ADF/cofilin), and side-binding proteins (fimbrin, 135-ABP/villin, 115-ABP). Although significant progress in documenting the biochemical activities of many of these classes of proteins has been made, the precise roles of actin-binding proteins in vivo awaits clarification by detailed mutational analyses.

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Profilin is associated with the plasma membrane in microspores and pollen

Cited by 35 publications

References 52 publications

Small Changes in the Regulation of One Arabidopsis Profilin Isovariant, PRF1, Alter Seedling Development

Small Changes in the Regulation of One Arabidopsis Profilin Isovariant, PRF1, Alter Seedling Development

Actin-Binding Proteins and Actin Dynamics in Plant Cells

Actin and actin-binding proteins in higher plants

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