Profilin and actin-depolymerizing factor: modulators of actin organization in plants

Staiger, Christopher J.; Gibbon, Bryan C.; Kovar, David R.; Zonia, Laura

doi:10.1016/s1360-1385(97)86350-9

Cited by 176 publications

(139 citation statements)

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“…The implication of actin filaments mediating intracellular nuclear movement is of particular interest given the known involvement of actin and actin-binding proteins in the regulation of plant cell shape (Heslop-Harrison et al, 1986;McCurdy and Williamson, 1991;Staiger et al, 1997;Szymanski et al, 1999). A variety of plant cell systems exhibiting various forms of polar growth have provided useful information, including stomatal development Wick, 1990, 1991), pollen germination (Picton and Steer, 1981;Heslop-Harrison et al, 1986;Lancelle et al, 1987;Miller et al, 1996;Kost et al, 1998), and trichome development (Szymanski et al, 1999).…”

Section: Involvement Of Microfilaments and Not Microtubules In Root Nmentioning

confidence: 99%

“…The situation in Drosophila may be different, with nuclear positioning in nurse cells and the syncytial embryo being reported to be exclusively actin associated (von Dassow and Schubiger, 1994;Guild et al, 1997). In one case in plants, i.e., tip-tracking within pollen (Heslop-Harrison and HeslopHarrison, 1989), movement was found to be driven by the interaction of actin with myosin associated with the surfaces of the vegetative nucleus and generative cell.The implication of actin filaments mediating intracellular nuclear movement is of particular interest given the known involvement of actin and actin-binding proteins in the regulation of plant cell shape (Heslop-Harrison et al, 1986;McCurdy and Williamson, 1991;Staiger et al, 1997;Szymanski et al, 1999). A variety of plant cell systems exhibiting various forms of polar growth have provided useful information, including stomatal development Wick, 1990, 1991), pollen germination (Picton and Steer, 1981;Heslop-Harrison et al, 1986;Lancelle et al, 1987;Miller et al, 1996;Kost et al, 1998), and trichome development (Szymanski et al, 1999).…”

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confidence: 99%

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Nuclear Dynamics inArabidopsis thaliana

Chytilová

Macas

Śliwińska

et al. 2000

MBoC

118

108

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The nucleus is a definitive feature of eukaryotic cells, comprising twin bilamellar membranes, the inner and outer nuclear membranes, which separate the nucleoplasmic and cytoplasmic compartments. Nuclear pores, complex macromolecular assemblies that connect the two membranes, mediate communication between these compartments. To explore the morphology, topology, and dynamics of nuclei within living plant cells, we have developed a novel method of confocal laser scanning fluorescence microscopy under time-lapse conditions. This is used for the examination of the transgenic expression in Arabidopsis thaliana of a chimeric protein, comprising the GFP (Green-Fluorescent Protein of Aequorea victoria) translationally fused to an effective nuclear localization signal (NLS) and to ␤-glucuronidase (GUS) from E. coli. This large protein is targeted to the nucleus and accumulates exclusively within the nucleoplasm.This article provides online access to movies that illustrate the remarkable and unusual properties displayed by the nuclei, including polymorphic shape changes and rapid, longdistance, intracellular movement. Movement is mediated by actin but not by tubulin; it therefore appears distinct from mechanisms of nuclear positioning and migration that have been reported for eukaryotes. The GFP-based assay is simple and of general applicability. It will be interesting to establish whether the novel type of dynamic behavior reported here, for higher plants, is observed in other eukaryotic organisms. INTRODUCTIONA definitive feature of eukaryotic cells is the nucleus, first described in stamen cells of Tradescantia by Robert Brown in 1831, which is found in all cell types at some stage of their development. The nucleus comprises twin bilamellar membranes, the inner and outer nuclear membranes, which serve to separate the nuclear contents, the nucleoplasm, from the cytoplasm. Communication between these compartments is mediated by nuclear pores, complex macromolecular assemblies that connect the two membranes.All of our information concerning nuclear morphology, topology, and dynamics comes from the use of various forms of microscopy. The major components of the nucleoplasm, including proteins, RNA, and DNA, do not absorb in the visible part of the spectrum. Thus, nuclei are nearly translucent and nonfluorescent. This impedes the observation of nuclei within living cells with standard bright-field or fluorescence microscopy. Although other forms of light microscopy can be used to examine nuclei, particularly phase contrast and differential interference contrast microscopy, which rely on optical properties other than absorbance and fluorescence, this is practical only in nonpigmented cells and in tissues having simple three-dimensional organization.We have developed an alternative method for observation of nuclei in living plant cells that uses confocal laser scanning fluorescence microscopy (Chytilova et al., 1999). Based on the transgenic expression of the Green Fluorescent Protein (GFP) of Aequorea victoria, it involves ...

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Section: Involvement Of Microfilaments and Not Microtubules In Root Nmentioning

confidence: 99%

mentioning

confidence: 99%

Nuclear Dynamics inArabidopsis thaliana

Chytilová

Macas

Śliwińska

et al. 2000

MBoC

118

108

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“…In plant cells, the small actin-binding proteins PFN and actindepolymerizing factor regulate actin dynamic, and a dynamic cytoskeleton in response to internal and external stimuli. PFNs interact with several other ligands: membrane polyphosphoinositides, stretches of PLP and proline-rich proteins, and these interactions probably serve to regulate PFN activity and to target it to specific locations where dynamic actin polymerization is necessary for cell function (Staiger et al 1997). In this study, PFN distributed in the cytoplasm and showed a punctuate pattern of localization around the periphery of nuclear envelopes in interphase and gathered toward the cell bipolar in prophase in roottip cells of wheat seedlings.…”

Section: Discussionmentioning

confidence: 60%

The distribution of profilin in root-tip cells of wheat seedlings exposed to enhanced UV-B radiation

Liu¹,

Tie²,

Chen³

et al. 2015

Frontiers in Life Science

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Actin filaments play a significant role in regulating the cell cycle. The dynamic rearrangement of actin filaments are regulated by actin-binding proteins profilin (PFN). However, the distribution of PFN in root-tip cells and the role of PFN in the UV response have been unknown. Here, we observed the distribution of PFN during every stage of wheat cell mitosis in the control and UV-B treatment group, and found that PFN showed a punctuate pattern of localization around the periphery of nuclear envelopes in interphase and gathered toward the cell bipolar in prophase in the control treatment group. During metaphase, PFN presented a cage shape that is formed around the chromosomes, then appeared in the equator during anaphase, and re-distributed around the periphery of nuclear envelopes during cytokinesis. But PFN localization had changed under enhanced UV-B radiation (10.08 kJm -2 h -1 ), PFN displayed an annular distribution in interphase and, during metaphase, relatively large number of PFN distributed in one of the four corners or gathered in the four corners in the cell. However, two corners of PFN moved to the other two corners along the direction perpendicular to the cell-elongating axis during telophase. And these aberrant distributions of PFN were often associated with abnormal chromosome distribution. ARTICLE HISTORY

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“…Microfilament is found in a steady state, at which the dissociation of G-actin from the "-" end and G-actin attachment to the "+" end are balanced (Staiger, Gibbon, Kovar, and Zonia, 1997;Blanchoin et al, 2010).…”

Section: Actin Cytoskeleton Organizationmentioning

confidence: 99%

Visualization and analysis of actin cytoskeleton organization in plants

Pozhvanov

2018

BioComm

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The plant cytoskeleton is a highly dynamic system that consists of two components: microfilaments and microtubules. Actin microfilaments are essential for polar growth, cytoplasmic streaming, directing polar growth, anchoring the nucleus, gravity sensing, signalling pathway integration and a number of other functions. Actin morphology and dynamics are orchestrated by a variety of small actin binding proteins, and some of them have become a source of actin interaction domains widely used as markers for microfilaments in fusions with fluorescent reporter proteins. However, older techniques are still employed for actin visualization. In this short review, we will focus on the diversity of fluorescent reporter fusions for F-actin and on approaches and existing free software for the analysis of cytoskeleton organization, mainly in Arabidopsis.

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Profilin and actin-depolymerizing factor: modulators of actin organization in plants

Cited by 176 publications

References 40 publications

Nuclear Dynamics inArabidopsis thaliana

Nuclear Dynamics inArabidopsis thaliana

The distribution of profilin in root-tip cells of wheat seedlings exposed to enhanced UV-B radiation

Visualization and analysis of actin cytoskeleton organization in plants

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