Profilin: A relevant aeroallergen?

Ruíz-García, Mónica; Potro, Manuela García del; Fernández‐Nieto, Mar; Barber, Domingo; Jimeno-Nogales, Lucía; Sastre, Joaquı́n

doi:10.1016/j.jaci.2011.03.008

Cited by 48 publications

(47 citation statements)

References 10 publications

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“…These observations are in keeping with clinical data from our group showing that most polcalcin reactors have symptoms mainly in spring (8) and confirm previous findings by Tinghino et al [14] showing that Phl p 7 is the strongest IgE-binding polcalcin. Variations in polcalcin content between pollens, along with the burden of each pollen in this geographic area, might explain how patients become sensitized to polcalcins, as was previously shown for profilin [17]. Furthermore, previous studies found that sensitization to polcalcins correlates both with a higher number of primary pollen sensitizations and with disease duration [18].…”

Section: Resultssupporting

confidence: 57%

IgE Reactivity to Polcalcins Varies According to Pollen Source

Asero¹,

Mistrello²,

Amato³

2016

J Investig Allergol Clin Immunol

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Background: Polcalcins are highly cross-reactive pollen panallergens. Less than 10% of allergic patients are sensitized to polcalcins. All pollen species are considered able to sensitize patients to this panallergen. Objective: We aimed to assess the presence of polcalcins in various pollen extracts used in allergen immunotherapy. Methods: ELISA inhibition experiments were performed with sera from patients sensitized to polcalcin and rPhl p 7 and rBet v 4. Recombinant polcalcin was used as the substrate and freshly prepared pollen extracts as inhibitors. Results: All pollen extracts induced significant inhibition of IgE reactivity to rBet v 4, whereas only grass pollen extract induced marked inhibition of IgE reactivity to rPhl p 7. Conclusion: Grass polcalcin probably contains more epitopes than polcalcins from other pollen sources. Grass pollen could be responsible for sensitization to polcalcins, and grass pollen immunotherapy is likely to be an option for polcalcin-hypersensitive patients.

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Section: Resultssupporting

confidence: 57%

IgE Reactivity to Polcalcins Varies According to Pollen Source

Asero¹,

Mistrello²,

Amato³

2016

J Investig Allergol Clin Immunol

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“…Panallergens generally do not seem to represent very important pollen sensitization components in Middle Europe, somewhat in contrast to southern countries where the observed sensitization frequency is often higher [14,28,37] and the clinical relevance of this sensitization may also be more important [38,39]. In our study, the highest observed sensitization rate was to profilins (fig.…”

Section: Discussioncontrasting

confidence: 59%

A Comprehensive Analysis of Middle-European Molecular Sensitization Profiles to Pollen Allergens

Panzner

Vachová

Vítovcová

et al. 2014

Int Arch Allergy Immunol

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Molecular diagnosis of allergy and microarray technology have opened a completely new avenue of insight into sensitization profiles from both the clinical and the epidemiological point of view. We used this innovative tool in the description of sensitization patterns in pollen-sensitized patients in Middle Europe. Immunoglobulin E detection using 112 different allergenic molecules was carried out employing the ImmunoCAP ISAC microarray system. Sera from 826 patients sensitized to at least one pollen-derived molecule were subjected to analysis. The highest observed sensitization rate was 81.0% to grass-specific molecules (the most frequent being Phl p 1; 69.6%). The second most frequent sensitization was 54.8% to Betulaceae-specific molecules (Bet v 1; 54.2%). Together, grasses and Betulaceae components (and their cosensitizations with other components) comprised the vast majority of pollen sensitizations. Unexpectedly frequently observed sensitizations were those to Cupressaceae-specific molecules (14.1%), Oleaceae-specific molecules (10.8%), and the plane tree-derived molecule Pla a 2 (15.5%). The sensitization rates for all other molecules were within the expected range (Art v 1, 13.6%; Pla l 1, 9.6%; Che a 1, 8.4%; Par j 2, 0.9%; Amb a 1, 0.8%, and Sal k 1, 0.5%). Cross-reacting molecule sensitization rates were found to be 12.4% for profilins, 5.0% for polcalcins, and 6.4% for lipid transfer proteins. Molecular diagnosis of allergy gives a more precise and comprehensive insight into pollen sensitization patterns than extract-based testing, allowing a better understanding of the sensitization process and regional differences. The data presented here may help to improve the diagnostic and allergen-specific treatment procedures in the respective region.

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“…Even though SPT solutions with profilin and polcalcin have been developed [21,22] and used as provocation agents [23,24], larger studies proving the clinical relevance of panallergens are lacking to date. …”

Section: Discussionmentioning

confidence: 99%

Clinical Relevance of IgE to Profilin and/or Polcalcin in Pollen-Sensitized Patients

Nicoló

Braun

Eder

et al. 2016

Int Arch Allergy Immunol

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Background: Component-resolved diagnostics is gaining importance in allergy diagnostics. Allergen extracts contain components with different rates of prevalence and clinical relevance, which can be subdivided at molecular level into major and minor allergens. Clinical complaints are usually triggered by major allergens, while the role of sensitization to the panallergens profilin and polcalcin still remains unclear. Methods: Eighty-six patients from southern Bavaria with sensitization to the panallergens profilin (Bet v 2/Phl p 12) and/or polcalcin (Bet v 4/Phl p 7) were examined in regard to their sensitization to the 4 main botanic denominations Betulaceae, Oleaceae, Poaceae and Asteraceae by skin prick test and measurement of specific immunoglobulin E antibodies to natural allergen extracts as well as major allergen components rPhl p 1/5, rBet v 1, rOle e 1 and nArt v 1. Sensitization was rated as clinically relevant or irrelevant depending on anamnesis or intranasal allergen challenge. Results: Regarding the 4 botanic denominations, there was no significant difference in the incidence of sensitization to the panallergens profilin, polcalcin or both. The sensitization pattern does not alter when subdividing the cohort into clinically relevant and silent sensitization. We did not find clinically symptomatic sensitization to panallergens without cosensitization to a major allergen. Conclusions: Our results suggest that sole sensitization to panallergens seems to have no clinical relevance in allergic rhinoconjunctivitis. Clinical complaints seem to be triggered manly by major allergens. Thus, component-resolved allergy diagnostics is crucial in the diagnosis and treatment of polysensitized patients.

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Profilin: A relevant aeroallergen?

Cited by 48 publications

References 10 publications

IgE Reactivity to Polcalcins Varies According to Pollen Source

IgE Reactivity to Polcalcins Varies According to Pollen Source

A Comprehensive Analysis of Middle-European Molecular Sensitization Profiles to Pollen Allergens

Clinical Relevance of IgE to Profilin and/or Polcalcin in Pollen-Sensitized Patients

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