Profilin 2 promotes migration, invasion, and stemness of HT29 human colorectal cancer stem cells

Kim, Min Jung; Lee, Yoo Sun; Han, Gi Yeon; Lee, Han Na; Ahn, Chiyoung; Kim, Chan Wha

doi:10.1080/09168451.2015.1043118

Cited by 31 publications

(24 citation statements)

References 41 publications

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“…Both PFN2 [51] and IQGAP2 [52] regulate cell motility in response to intracellular signals. PFN2 is an epigenetic regulator of SMAD2/3 (Mothers against decapentaplegic homolog 2/3) [53], which in turn has been shown to promote metastasis in cancer cells [54]. SMAD2 and SMAD3 are also regulated by HIF-1α [55].…”

Section: Protein Association Network Identify Signaling Components Pmentioning

confidence: 99%

Cyclosporine a directly affects human and mouse b cell migration in vitro by disrupting a hIF-1 αdependent, o2 sensing, molecular switch

et al. 2020

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Background: Hypoxia is a potent molecular signal for cellular metabolism, mitochondrial function, and migration. Conditions of low oxygen tension trigger regulatory cascades mediated via the highly conserved HIF-1α post-translational modification system. In the adaptive immune response, B cells (Bc) are activated and differentiate under hypoxic conditions within lymph node germinal centers, and subsequently migrate to other compartments. During migration, they traverse through changing oxygen levels, ranging from 1-5% in the lymph node to 5-13% in the peripheral blood. Interestingly, the calcineurin inhibitor cyclosporine A is known to stimulate prolyl hydroxylase activity, resulting in HIF-1α destabilization and may alter Bc responses directly. Over 60% of patients taking calcineurin immunosuppressant medications have hypo-gammaglobulinemia and poor vaccine responses, putting them at high risk of infection with significantly increased morbidity and mortality. Results: We demonstrate that O 2 tension is a previously unrecognized Bc regulatory switch, altering CXCR4 and CXCR5 chemokine receptor signaling in activated Bc through HIF-1α expression, and controlling critical aspects of Bc migration. Our data demonstrate that calcineurin inhibition hinders this O 2 regulatory switch in primary human Bc. Conclusion: This previously unrecognized effect of calcineurin inhibition directly on human Bc has significant and direct clinical implications.

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Section: Protein Association Network Identify Signaling Components Pmentioning

confidence: 99%

Cyclosporine a directly affects human and mouse b cell migration in vitro by disrupting a hIF-1 αdependent, o2 sensing, molecular switch

et al. 2020

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“…FOXD2-AS1 and PFN2 expression was positively correlated. Previous studies demonstrated that miR-150-5p and PFN2 were able to regulate the development of certain cancer types (33)(34)(35). miR-150-5p inhibits cancer cell aggressiveness by targeting SPARC (osteonectin), cwcv and kazal like domain proteoglycan 1 in head and neck squamous cell carcinoma (33).…”

Section: Discussionmentioning

confidence: 99%

Long non‑coding RNA FOXD2‑AS1/miR‑150‑5p/PFN2 axis regulates breast cancer malignancy and tumorigenesis

et al. 2019

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Breast cancer (BC) is a common cancer and leading cause of cancer-associated mortality in women. Abnormal expression of long non-coding RNA FOXD2 adjacent opposite strand RNA 1 (FOXD2-AS1) was associated with the development of a number of tumors. However, whether FOXD2-AS1 is dysregulated in BC and its underlying mechanisms remain unclear. In the present study, it was identified that FOXD2-AS1 expression was upregulated in BC tissue, cell lines and sphere subpopulation. Additionally, the abnormal upregulation of FOXD2-AS1 predicted poor prognosis in patients with BC. Furthermore, downregulation of FOXD2-AS1 decreased cell proliferation, and migratory and invasive abilities in BC cells, and decreased the growth of transplanted tumors in vivo. Downregulation of FOXD2-AS1 decreased the percentage of CD44 antigen + /signal transducer CD24in breast cancer stem cell (BCSC) cells, and decreased the expression of numerous stem factors, including Nanog, octamer-binding transcription factor 4 (Oct4), and sex determining region Y-box 2 (SOX2), and inhibited the epithelial-mesenchymal transition process. FOXD2-AS1 was identified to be primarily located in the cytoplasm. Using bioinformatics analysis, a reporter gene assay and reverse transcription-polymerase chain reaction assays, it was demonstrated that microRNA (miR)-150-5p was able to bind directly with the 3'-untranslated region of FOXD2-AS1 and PFN2 mRNA. miR-150-5p mimics decreased the cell proliferation, migration and invasion of BC cells. FOXD2-AS1 knockdown significantly inhibited the miR-150-5p inhibitor-induced increase in Nanog, Oct4 and SOX2 expression. The miR-150-5p inhibitor-induced increase in N-cadherin, and decrease in E-cadherin and vimentin was inhibited by FOXD2-AS1 knockdown. Profilin 2 (PFN2) expression was significantly upregulated in BC tissues. Additionally, the abnormal upregulation of PFN2 was associated with poor prognosis in patients with BC. FOXD2-AS1 and PFN2 expression was positively correlated. Collectively, the present results demonstrated the role of the FOXD2-AS1/miR-150-5p/PFN2 axis in the development of BC, and provides novel targets for the treatment of BC, and potential biomarkers for diagnosis and prognosis of BC.

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“…The invasion assay was performed at 37 °C in a humidified atmosphere with 5% for 48 or 72 h. After incubation, the invasion assay was performed as the migration assay. Invasiveness was determined by counting cells with image J in three randomly selected microscopic fields per well …”

Section: Methodsmentioning

confidence: 99%

“…Invasiveness was determined by counting cells with image J in three randomly selected microscopic fields per well. [20][21][22]…”

Section: Migration and Invasion Assaysmentioning

confidence: 99%

Targeting ROR1 inhibits the self‐renewal and invasive ability of glioblastoma stem cells

Jung

Lee

Han

et al. 2016

Cell Biochemistry & Function

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Glioblastoma is the most malignant of brain tumours and is difficult to cure because of interruption of drug delivery by the blood-brain barrier system, its high metastatic capacity and the existence of cancer stem cells (CSCs). Although CSCs are present as a small population in malignant tumours, CSCs have been studied as they are responsible for causing recurrence, metastasis and resistance to chemotherapy and radiotherapy for cancer. CSCs have self-renewal characteristics like normal stem cells. The aim of this study was to investigate whether receptor tyrosine kinase-like orphan receptor 1 (ROR1) is involved in stem cell maintenance and malignant properties in human glioblastoma. Knockdown of ROR1 caused reduction of stemness and sphere formation capacity. Moreover, down-regulation of ROR1 suppressed the expression of epithelial-mesenchymal transition-related genes and the tumour migratory and invasive abilities. The results of this study indicate that targeting ROR1 can induce differentiation of CSCs and inhibit metastasis in glioblastoma. In addition, ROR1 may be used as a potential marker for glioblastoma stem cells as well as a potential target for glioblastoma stem cell therapy.

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Profilin 2 promotes migration, invasion, and stemness of HT29 human colorectal cancer stem cells

Cited by 31 publications

References 41 publications

Cyclosporine a directly affects human and mouse b cell migration in vitro by disrupting a hIF-1 αdependent, o2 sensing, molecular switch

Cyclosporine a directly affects human and mouse b cell migration in vitro by disrupting a hIF-1 αdependent, o2 sensing, molecular switch

Long non‑coding RNA FOXD2‑AS1/miR‑150‑5p/PFN2 axis regulates breast cancer malignancy and tumorigenesis

Targeting ROR1 inhibits the self‐renewal and invasive ability of glioblastoma stem cells

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