Profilin 1: Do we have a novel proteome-found biomarker predicting response to anticancer therapy?

Skvortsova, Ira

doi:10.1002/pmic.201300165

Cited by 6 publications

(5 citation statements)

References 7 publications

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“…We also note our result strengthens the premise of Peng's paper which was that actin binding proteins may play important roles in head and neck lymph node invasion. These findings agree with another report from HNSCC cell lines that induction of reduced PFN1 expression coincided with increased in vitro cell migration .…”

Section: Discussionsupporting

confidence: 93%

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A loss of profilin‐1 in late‐stage oral squamous cell carcinoma

Adami

O'Callaghan

Kolokythas

et al. 2016

J Oral Pathology Medicine

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BACKGROUND The genes for PFN1 and TMSB4 are both highly expressed in oral tissue and both encode actin monomer binding proteins thought to play a role in cell motility and possibly other crucial parts of tumor progression. METHODS Oral brush cytology of epithelium from Oral Squamous Cell Carcinoma (OSCC) was used to measure PFN1 and TMSB4 mRNA in OSCC while immunohistochemical analysis of tissue was used to check protein levels. RESULTS High but variable expression of mRNAs encoding these two proteins was observed suggesting they may contribute to tumor characteristics in a subset of OSCCs. Both proteins were highly expressed in normal appearing basal epithelium, in the cytoplasm and perinuclear area, while expression was minimal in upper epithelial layers. In OSCCs, expression of these proteins varied. In tumors classified as later stage, based on size and/or lymph node involvement, PFN1 levels were lower in tumor epithelium. A control gene, KRT13, showed expression in normal differentiated basal and suprabasal oral mucosa epithelial cells and as reported was lost in OSCC cells. CONCLUSION Loss of PFN1in tumor cells has been associated with lymph node invasion and metastasis in other tumor types, strengthening the argument that the protein has the potential to be a tumor suppressor in late stage OSCC.

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Section: Discussionsupporting

confidence: 93%

“…These findings agree with another report from HNSCC cell lines that induction of reduced PFN1 expression coincided with increased in vitro cell migration (29). The majority of tumors we reviewed showed elevated expression of TMSB4 vs. normal appearing tissue, consistent with its identification in other tumor types as an oncoprotein (Fig.…”

Section: Discussionsupporting

confidence: 92%

A loss of profilin‐1 in late‐stage oral squamous cell carcinoma

Adami

O'Callaghan

Kolokythas

et al. 2016

J Oral Pathology Medicine

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“…Profilin, on the other hand, prevents DM progression [ 84 ]. Furthermore, profilin is also considered as a biomarker for cancer as it plays a key role in actin assembly and microtubule dynamics [ 85 ]. In eukaryotes multiple isoforms of profilin exists.…”

Section: Discussionmentioning

confidence: 99%

Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics

et al. 2022

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The present research investigates the tuber proteome of the ‘medicinal’ plant Jerusalem artichoke (abbreviated as JA) (Helianthus tuberosus L.) using a high-throughput proteomics technique. Although JA has been historically known to the Native Americans, it was introduced to Europe in the late 19th century and later spread to Japan (referred to as ‘kiku-imo’) as a folk remedy for diabetes. Genboku Takahashi research group has been working on the cultivation and utilization of kiku-imo tuber as a traditional/alternative medicine in daily life and researched on the lowering of blood sugar level, HbA1c, etc., in human subjects (unpublished data). Understanding the protein components of the tuber may shed light on its healing properties, especially related to diabetes. Using three commercially processed JA tuber products (dried powder and dried chips) we performed total protein extraction on the powdered samples using a label-free quantitate proteomic approach (mass spectrometry) and catalogued for the first time a comprehensive protein list for the JA tuber. A total of 2967 protein groups were identified, statistically analyzed, and further categorized into different protein classes using bioinformatics techniques. We discussed the association of these proteins to health and disease regulatory metabolism. Data are available via ProteomeXchange with identifier PXD030744.

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“…Currently, the PKC inhibitor drugs available in the market are toxic; therefore, profilin-like protein extracted from P. pulmonarius could be a starting point to an alternative treatment for DM. Drug development in this area is important because it not only offers new treatment strategy for DM but also contributes to therapeutic options for cancer, autoimmune disease, and inflammation because it also acts as a tumour suppressor in human carcinomas [37]. …”

Section: Discussionmentioning

confidence: 99%

Characterisation of Potential Antidiabetic-Related Proteins fromPleurotus pulmonarius(Fr.) Quél. (Grey Oyster Mushroom) by MALDI-TOF/TOF Mass Spectrometry

Wahab

Abdullah

Aminudin

2014

BioMed Research International

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Pleurotus pulmonarius has been reported to have a potent remedial effect on diabetic property and considered to be an alternative for type 2 diabetes mellitus treatment. This study aimed to investigate the antidiabetic properties of ammonium sulphate precipitated protein fractions from P. pulmonarius basidiocarps. Preliminary results demonstrated that 30% (NH4)2SO4 precipitated fraction (F30) inhibited Saccharomyces cerevisiae α-glucosidase activity (24.18%), and 100% (NH4)2SO4 precipitated fraction (F100) inhibited porcine pancreatic α-amylase activity (41.80%). Following RP-HPLC purification, peak 3 from F30 fraction demonstrated inhibition towards α-glucosidase at the same time with meagre inhibition towards α-amylase activity. Characterisation of proteins using MALDI-TOF/TOF MS demonstrated the presence of four different proteins, which could be implicated in the regulation of blood glucose level via various mechanisms. Therefore, this study revealed the presence of four antidiabetic-related proteins which are profilin-like protein, glyceraldehyde-3-phosphate dehydrogenase-like protein, trehalose phosphorylase-like (TP-like) protein, and catalase-like protein. Hence, P. pulmonarius basidiocarps have high potential in lowering blood glucose level, reducing insulin resistance and vascular complications.

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Profilin 1: Do we have a novel proteome-found biomarker predicting response to anticancer therapy?

Cited by 6 publications

References 7 publications

A loss of profilin‐1 in late‐stage oral squamous cell carcinoma

A loss of profilin‐1 in late‐stage oral squamous cell carcinoma

Unravelling the Helianthus tuberosus L. (Jerusalem Artichoke, Kiku-Imo) Tuber Proteome by Label-Free Quantitative Proteomics

Characterisation of Potential Antidiabetic-Related Proteins fromPleurotus pulmonarius(Fr.) Quél. (Grey Oyster Mushroom) by MALDI-TOF/TOF Mass Spectrometry

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