Profile of polyunsaturated fatty acids produced by <i>Thraustochytrium</i> sp. KK17‐3

Huang, Jianzhong; Aki, Tsunehiro; Hachida, Kazutaka; Yokochi, Toshihiro; Kawamoto, Seiji; Shigeta, Seiko; Ono, Kazuhisa; Сузукі, Осаму

doi:10.1007/s11746-001-0312-1

Cited by 41 publications

(16 citation statements)

References 29 publications

(33 reference statements)

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“…In a strain of this species, lipid, TAG and PUFA contents appeared to reach the highest values during the early phase of cell growth, with DHA representing about 10% of total lipids [117]. Huang et al [118] described a Thraustochytrium sp. strain containing 52% of total fatty acids as DHA, as well as other LC-PUFA (AA, EPA, n -6 DPA), which accounted for a total of 76% of total fatty acids.…”

Section: Microalgae As Sources Of N-3 Lc-pufamentioning

confidence: 99%

Alternative Sources of n-3 Long-Chain Polyunsaturated Fatty Acids in Marine Microalgae

et al. 2013

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The main source of n-3 long-chain polyunsaturated fatty acids (LC-PUFA) in human nutrition is currently seafood, especially oily fish. Nonetheless, due to cultural or individual preferences, convenience, geographic location, or awareness of risks associated to fatty fish consumption, the intake of fatty fish is far from supplying the recommended dietary levels. The end result observed in most western countries is not only a low supply of n-3 LC-PUFA, but also an unbalance towards the intake of n-6 fatty acids, resulting mostly from the consumption of vegetable oils. Awareness of the benefits of LC-PUFA in human health has led to the use of fish oils as food supplements. However, there is a need to explore alternatives sources of LC-PUFA, especially those of microbial origin. Microalgae species with potential to accumulate lipids in high amounts and to present elevated levels of n-3 LC-PUFA are known in marine phytoplankton. This review focuses on sources of n-3 LC-PUFA, namely eicosapentaenoic and docosahexaenoic acids, in marine microalgae, as alternatives to fish oils. Based on current literature, examples of marketed products and potentially new species for commercial exploitation are presented.

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Section: Microalgae As Sources Of N-3 Lc-pufamentioning

confidence: 99%

Alternative Sources of n-3 Long-Chain Polyunsaturated Fatty Acids in Marine Microalgae

et al. 2013

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“…Marine microorganisms belonging to achromatic stramenopiles such as thraustochytrids and labyrinthulids have been reported to produce significant quantities of PUFAs [5]. Recent studies have shown that some thraustochytrid strains can produce high biomass, containing substantial amounts of lipid rich PUFAs.…”

Section: Introductionmentioning

confidence: 99%

Lipid body formation by Thraustochytrium aureum (ATCC 34304) in response to cell age

Jeh¹,

Kumaran²,

Hur³

2008

Korean J. Chem. Eng.

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The heterotrophic marine protist, Thraustochytrium aureum produces substantial amounts of polyunsaturated fatty acids (PUFAs). In the present investigation, changes in the lipid and fatty acid profiles of T. aureum were studied according to the culture age. T. aureum was grown in artificial sea water medium for 10 days at 25 o C in shake culture condition. One to 10 day old cell samples were analyzed for cell biomass production, total lipid content, fatty acid profile and lipid body formation. In all the samples tested, total lipid production was found to be directly proportional to the dry cell weight of T. aureum. In the early phase of cell growth, cell biomass production, lipid content and glucose consumption were found to be higher. Thin layer chromatographic analysis (TLC) of lipids showed the presence of triacylglycerol (TAG; 169 mg/g, 90%), phospholipids (PL; 83 mg/g, 66%) and sterol (ST; 6 mg/g, 5%), which were recorded at maximum levels in the early growth phase of the cells. The composition of PUFAs and saturated fatty acids (SFAs) of the cell biomass and lipid class components (TAG and PL) was identified by gas chromatographic analysis (GC). In the early phase of cell growth, production of PUFAs in the total fatty acids was found to have attained maximum levels (61.3%) in which docosahexaenoic acid alone showed higher content of occurrence (99.0 mg/g in total lipid; 65.2 mg/g in TAG and 41.0 mg/g in PL). In the middle phase of cell growth, palmitic acid production was found to be higher (36.7 mg/g in total lipid; 31.3 mg/g in TAG and 12.6 mg/g in PL). Transmission electron microscopic studies of the cells showed the presence of a membrane around the lipid bodies in the early phase of cell growth. TAG and PL were actively involved in the formation of lipid bodies in the cells of T. aureum. Large-sized lipid bodies accumulated in 3 day old cells which were then fragmented into smaller bodies in the late growth phase.

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“…Even though glucose is generally the first CS used for evaluating DHA production by thraustochytrids it has been reported that for some strains, especially Thraustochytrium strains, better results are obtained with starch and maltose . Various peptones are good NS for the production of thraustochytrid biomass while few strains are able to utilize efficiently inorganic sources like ammonium and nitrate . The fact that biomass production by Thraustochytriidae sp.…”

Section: Discussionmentioning

confidence: 99%

Production of lipids and docosahexasaenoic acid (DHA) by a native Thraustochytrium strain

Shene

Leyton

Rubilar

et al. 2013

Euro J Lipid Sci & Tech

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Production of docosahexasaenoic acid (DHA) by a native Labyrinthulomycetes strain, Thraustochytriidae sp. TN5, whose growth characteristics present some differences to related strains, was scaled from shaken flask to a laboratory fermentor. The effect of the growth medium composition and growth conditions for (i) biomass production, (ii) lipid content of the biomass, and (iii) DHA content in the lipids was determined. Taguchi's design of experiments was used to study the influence of two discrete -carbon and nitrogen sources -and six continuous -concentrations of the carbon and nitrogen sources, yeast extract and artificial seawater, incubation temperature, and time-factors on the three mentioned variables. In the flask experiments the lipidic content in the biomass (25.2% w/w) and DHA concentration (0.48 g/L) were the highest at the following conditions: maltose 20 g/L, sodium glutamate 2.4 g/L, yeast extract 6 g/L, 72 h, 258C, and artificial seawater 30% v/v; under these conditions biomass concentration was 5.1 g/L. Fed-batch allowed to increase biomass concentration to 14 g/L. The lipidic fraction of Thraustochytriidae sp. TN5 biomass in the repeated batches was found between 16.2 and 34.8% w/w. Under this growth condition lipids and DHA productivities were 50 and 23 mg/(L h), respectively.Practical applications: Consumption of long chain-polyunsaturated fatty acids (LC-PUFA) belonging to the omega-3 family such as DHA has several positive effects on human and animal health. However, natural sources are restricted to cold-water fish and their oils. Marine protists are also good candidates for the production of microbial DHA. For evaluating the potential of new strains the effect of the growth medium composition, growth conditions, and cultivation mode on DHA productivity has to be determined. In this work the production of DHA by a native Labyrinthulomycetes strain was scaled from shaken flask to a laboratory fermentor with the aid of Taguchi's methodology, process in which biomass concentration, lipid content in the biomass, and DHA content in the lipids were increased.Abbreviations: C, concentration of the carbon source; CS, carbon source; DHA, docosahexasaenoic acid; DO, dissolved oxygen; DOE, design of experiments; FAME, fatty acid methyl ester; LC-PUFA, long chainpolyunsaturated fatty acids; N, concentration of the nitrogen source;

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Profile of polyunsaturated fatty acids produced by Thraustochytrium sp. KK17‐3

Cited by 41 publications

References 29 publications

Alternative Sources of n-3 Long-Chain Polyunsaturated Fatty Acids in Marine Microalgae

Alternative Sources of n-3 Long-Chain Polyunsaturated Fatty Acids in Marine Microalgae

Lipid body formation by Thraustochytrium aureum (ATCC 34304) in response to cell age

Production of lipids and docosahexasaenoic acid (DHA) by a native Thraustochytrium strain

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