Profile of native cellulosomal proteins of Clostridium cellulovorans adapted to various carbon sources

Abstract

We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [Tamaru, Y., et al.. 2010. J. Bacteriol. 192:901–902]. Our system employed a long monolithic column (300 cm), which provides better performance and higher resolution than conventional systems. Twenty-three cellulosomal proteins were, without purification, identified by direct analysis of the culture medium. Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of carbon source-adapted cellulosome components.

Exoproteome Profiles of Clostridium cellulovorans Grown on Various Carbon Sources

“…The average of the spectral counts of the cellulosomal proteins was much larger than that of the noncellulosomal proteins (Table 1). In addition, a scaffolding protein of the cellulosome (Clocel_ 2824) showed the highest counts, and basic cellulosomal proteins (Clocel_2823 and Clocel_3359), which are reported to be constitutively produced (13), and another cellulosomal protein produced in xylan culture (Clocel_2821) also showed high counts (third, fourth, and second, respectively) (see Table S2 in the supplemental material). The results indicate that the cellulosomal proteins were more highly produced than the noncellulosomal proteins.…”

“…Proteome analyses were performed using a liquid chromatography-mass spectrometry system equipped with a long monolithic silica capillary column, as described in our previous study (13). A monolithic silica capillary column was prepared from a mixture of tetramethoxysilane and methyltrimethoxysilane (20).…”

“…However, it is important to note that C. cellulovorans must utilize noncellulosomal proteins for polysaccharide degradation under native conditions (13). There have been several studies about noncellulosomal proteins from either C. thermocellum or C. cellulovorans using gel-based methods, but they were limited to only a small fraction of the noncellulosomal proteins (14,15).…”

Advances in consolidated bioprocessing systems for bioethanol and butanol production from biomass: a comprehensive review

“…The hemicellulosic portion of lignocelluloses are more complex and is commonly composed of several backbones like xyloglucan, glucomannan, glucuronoxylan, etc., depending on the type of plant residue (Parisutham et al, 2014). So, an ideal CBP microorganism, similar to native cellulolytic microorganisms, should possess all sets of cellulases and should be able to grow on different available carbon sources (Morisaka et al, 2012). Highthroughput screening technologies and metagenomics have been established to screen for better enzymes that can reduce the requirements for a large number of cellulases (Peralta-Yahya et al, 2008).…”

Combining Proteomic Strategies and Molecular Display Technology for Development of Vaccines against Candida albicans

“…C. albicans incubated in YNB ± FBS media for 0-60 min could provide a good model to investigate the dynamics of proteome variation in the early stages of infection. In the next step, prepared peptides derived from proteins identified from C. albicans grown in YNB ± FBS media were subjected to LC-MS/MS measurement using a long monolithic silica capillary column (200 cm) [16], and a lot of proteins were successfully identified. Aoki et al [15] identified 1130, 1012, and 701 proteins from the 0, 60 min cultures in YNB −FBS, and 60 min of YNB +FBS samples.…”