Profile of Gut Microbiota Associated With the Presence of Hepatocellular Cancer in Patients With Liver Cirrhosis

Grąt, Michał; Wronka, Karolina Maria; Krasnodębski, Maciej; Masior, Łukasz; Lewandowski, Zbigniew; Kosińska, Irena; Grąt, Karolina; Stypułkowski, Jan; Rejowski, Sławomir; Wasilewicz, Michał; Gałęcka, Mirosława; Szachta, Patrycja; Krawczyk, Marek

doi:10.1016/j.transproceed.2016.01.077

Cited by 161 publications

(117 citation statements)

References 30 publications

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“…One recent report performed a metagenomic study on fecal samples from liver cirrhosis patients with or without HCC and the result showed that although there was no significant differences on the fecal counts of Enterobacteriaceae, Enterococcus species, Bifidobacterium species, Bacteroides species, Lactobacillus species and Clostridium species between them32, but the levels of Escherichia coli was significantly higher in HCC patients, which was believed to be able to deconjugate conjugated BAs to form secondary BAs1533. The conversion of primary to secondary BAs and de-conjugation of BAs into free BAs are also attributed to bacteria Clostridium, Eubacterium, Bifidobacterium and Lactobacillus .…”

Section: Discussionmentioning

confidence: 99%

Sex-dependent effects on gut microbiota regulate hepatic carcinogenic outcomes

Xie

Wang

Zhao

et al. 2017

Sci Rep

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Emerging evidence points to a strong association between sex and gut microbiota, bile acids (BAs), and gastrointestinal cancers. Here, we investigated the mechanistic link between microbiota and hepatocellular carcinogenesis using a streptozotocin-high fat diet (STZ-HFD) induced nonalcoholic steatohepatitis-hepatocellular carcinoma (NASH-HCC) murine model and compared results for both sexes. STZ-HFD feeding induced a much higher incidence of HCC in male mice with substantially increased intrahepatic retention of hydrophobic BAs and decreased hepatic expression of tumor-suppressive microRNAs. Metagenomic analysis showed differences in gut microbiota involved in BA metabolism between normal male and female mice, and such differences were amplified when mice of both sexes were exposed to STZ-HFD. Treating STZ-HFD male mice with 2% cholestyramine led to significant improvement of hepatic BA retention, tumor-suppressive microRNA expressions, microbial gut communities, and prevention of HCC. Additionally the sex-dependent differences in BA profiles in the murine model can be correlated to the differential BA profiles between men and women during the development of HCC. These results uncover distinct male and female profiles for gut microbiota, BAs, and microRNAs that may contribute to sex-based disparity in liver carcinogenesis, and suggest new possibilities for preventing and controlling human obesity-related gastrointestinal cancers that often exhibit sex differences.

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Section: Discussionmentioning

confidence: 99%

Sex-dependent effects on gut microbiota regulate hepatic carcinogenic outcomes

Xie

Wang

Zhao

et al. 2017

Sci Rep

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“…20 Only one other human study has profiled the gut microbiota in HCC (not limited to NAFLD-related HCC) and found higher levels of Escherichia coli through a culture-based approach. 29 …”

Section: Human Gut Microbiome Profiles In Clinical Phenotypes Of Naflmentioning

confidence: 99%

Emerging Role of the Gut Microbiome in Nonalcoholic Fatty Liver Disease: From Composition to Function

Sharpton

Ajmera

Loomba

2019

Clinical Gastroenterology and Hepatology

135

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The gut microbiome, a diverse microbial community in the gastrointestinal tract, plays a pivotal role in the maintenance of health. The gut microbiome metabolizes dietary and host-derived molecules to produce bioactive metabolites, which have a wide array of effects on host metabolism and immunity. 'Dysbiosis' of the gut microbiome, commonly considered as perturbation of microbiome diversity and composition, has been associated with intestinal and extra-intestinal diseases, including nonalcoholic fatty liver disease (NAFLD). A number of endogenous and exogenous factors, such as nutritional intake and xenobiotic exposure, can alter the gut microbiome. We will review the evolving methods for studying the gut microbiome and how these profiling techniques have been utilized to further our understanding of the gut microbial community composition and functional potential in the clinical spectrum of NAFLD. We will highlight microbiome-host interactions that may contribute to the pathogenesis of NAFLD, with a primary focus on mechanisms related to the metabolic output of the gut microbiome. Finally, we will discuss potential therapeutic implications of the gut microbiome in NAFLD.

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“…Ahluwalia et al (2016) enrolled 40 controls and 147 cirrhotic patients (87 with hepatic encephalopathy), and researched on the relationship between stool microbiota and brain magnetic resonance imaging (MRI), with the results shown negative linkages between autochthonous families and positive ones between Enterobacteriaceae to MR spectroscopy and hyperammonemia-associated astrocytic changes. Grąt et al (2016) analyzed the gut microbial profile from 15 patients with hepatocellular cancer (HCC) and 15 non-HCC patients, which suggested that the overgrowth of E coli in the gut may contribute to the process of hepatocarcinogenesis. Usami et al (2013) collected fecal samples from 46 hepatic cancer patients, followed by the evaluation of fecal constituents and lipid and fatty acid concentrations, and suggested that intestinal microbiota affected serum fatty acid metabolism and were modified by liver disorders.…”

Section: Introductionmentioning

confidence: 99%

Community-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B Patients

et al. 2016

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The gut flora are widely involved in the cometabolism with the host and have evident effects on the metabolic phenotype of host. This study performed a metabolome analysis of the intestinal microbiota specific for liver cirrhosis. The study population included patients with Child-Turcotte-Pugh score of A (AP, n = 5) and B (BP, n = 5), and control subjects (NM, n = 3). Metagenomic DNA from fecal microbiota was extracted followed by metagenomic sequencing through Illumina MiSeq high throughput sequencing of 16S rRNA regions. The detection of metabolites from fecal samples was performed using high-performance liquid phase chromatography and gas chromatography coupled with tandem mass spectrometry. Intestinal microbiota community and metabolite analysis both showed separation of cirrhotic patients from control participants, moreover, the microbiota–metabolite correlations changed in cirrhotic patients. Fecal microbiota from cirrhotic patients, with the reduced diversity, contained a decreased abundance of Bacteroidetes and an increased abundance of Firmicutes and Proteobacteria compared with the normal samples. Analysis of metabolome revealed a remarkable change in the metabolic potential of the microbiota in cirrhotic patients, with specific higher concentrations of amine, unsaturated fatty acid, and short-chain fatty acids, and lower concentrations of sugar alcohol and amino acid, suggesting the initial equilibrium of gut microbiota community and co-metabolism with the host were perturbed by cirrhosis. Our study illustrated the relationship between fecal microbiota composition and metabolome in cirrhotic patients, which may improve the clinical prognosis of cirrhosis.

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Profile of Gut Microbiota Associated With the Presence of Hepatocellular Cancer in Patients With Liver Cirrhosis

Cited by 161 publications

References 30 publications

Sex-dependent effects on gut microbiota regulate hepatic carcinogenic outcomes

Sex-dependent effects on gut microbiota regulate hepatic carcinogenic outcomes

Emerging Role of the Gut Microbiome in Nonalcoholic Fatty Liver Disease: From Composition to Function

Community-Metabolome Correlations of Gut Microbiota from Child-Turcotte-Pugh of A and B Patients

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