Profile of Ets gene expression in human breast carcinoma

He, Jin; Pan, Yong; Hu, Jianhua; Albarracin, Constance T.; Wu, Yun; Dai, Jia Le

doi:10.4161/cbt.6.1.3551

Cited by 49 publications

(64 citation statements)

References 34 publications

(37 reference statements)

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“…Alternatively, since the anti-PDEF antibodies used in two of the three previous studies (19,20) were generated against the full-length PDEF protein, they carried the potential for crossreaction with other Ets factors in the immunohistochemical assay. The latter potential stems from: i) the high degree of sequence homology approaching 45 to 55% amino acid sequence identity in the DNA binding domain between PDEF and other Ets factors (11); and ii) the large number of Ets factors that are expressed in normal mammary gland, and several at much higher levels than Pse or PDEF (17,22). The third study (21) used antibody raised against the same N-terminal segment of PDEF as used in this study, however, the specificity of the immunohistochemical assay for PDEF was not demonstrated in that study.…”

Section: Discussionmentioning

confidence: 99%

Expression characteristics of prostate-derived Ets factor support a role in breast and prostate cancer progression

et al. 2007

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The purpose of this study was to understand the characteristics of PDEF protein expression in breast and prostate cancer progression. A polyclonal antibody specific to PDEF was raised and reacted with tissue microarrays consisting of benign breast, in situ ductal, invasive ductal and invasive lobular breast carcinomas. The antibody was also reacted with tissue microarrays including benign prostate, prostate intra-epithelial neoplasias and prostate carcinomas. Increased expression of PDEF was identified in 18%, 50%, 46% and 51% of benign breast tissues, intraductal, invasive ductal and invasive lobular carcinomas, respectively. Importantly, in matched samples of benign breast versus tumor, 90% showed higher expression of PDEF in the tumor tissue. Moreover, in invasive breast carcinomas, increased PDEF expression tended to correlate with Her2/neu over expression. Increased expression of PDEF was also found in 27%, 33% and 40% of benign prostate tissues, PIN samples and prostate adenocarcinomas, respectively. Again, in matching samples of cancer versus benign and cancer versus PIN, 68% and 70% respectively showed increased expression in the malignant tissue. Moreover, PDEF was found to be more highly expressed in tumors with intermediate or high Gleason score compared to low grade tumors (P<0.01). Additionally, R1881 treatment induced PDEF expression in the LNCaP prostate tumor cell line, suggesting regulation of PDEF by androgens in vivo. Together, these results for the first time show frequent increased expression of PDEF protein in breast and prostate tumors and support a role for PDEF in breast and prostate cancer progression.

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Section: Discussionmentioning

confidence: 99%

Expression characteristics of prostate-derived Ets factor support a role in breast and prostate cancer progression

et al. 2007

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“…20 EHF has been shown to be differentially regulated in prostate, serous ovarian, and breast carcinomas. [19][20][21] S100A8 is a calciumbinding protein that was also upregulated in our study comparing squamous cell carcinoma to pseudoepitheliomatous hyperplasia and squamous cell carcinoma to normal skin. 8,9 It is involved in the regulation of various cellular processes, and its role in tumorigenesis was previously discussed.…”

Section: Discussionmentioning

confidence: 99%

Keratoacanthoma and squamous cell carcinoma are distinct from a molecular perspective

Seong

et al. 2015

Modern Pathology

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Keratoacanthoma is a controversial entity. Some consider keratoacanthoma as a variant of squamous cell carcinoma, whereas others see it as a distinct self-resolving squamoproliferative lesion. Our objective is to examine the relationship of keratoacanthoma with squamous cell carcinoma and normal skin by using DNA microarrays. DNA microarray studies were performed on formalin-fixed and paraffin-embedded blocks from ten cases of actinic keratoacanthoma utilizing the U133plus2.0 array. These results were compared with our previously developed microarray database of ten squamous cell carcinoma and ten normal skin samples. Keratoacanthoma demonstrated 1449 differentially expressed genes in comparison with squamous cell carcinoma (45-fold change: Po0.01) with 908 genes upregulated and 541 genes downregulated. Keratoacanthoma showed 2435 differentially expressed genes in comparison with normal skin (45-fold change: Po0.01) with 1085 genes upregulated and 1350 genes downregulated. The most upregulated genes, comparing keratoacanthoma with normal skin included MALAT1, S100A8, CDR1, TPM4, and CALM1. The most downregulated genes included SCGB2A2, DCD, THRSP, ADIPOQ, adiponectin, and ADH1B. The molecular biological pathway analysis comparing keratoacanthoma with normal skin showed that cellular development, cellular growth and proliferation, cell death/apoptosis, and cell cycle pathways are prominently involved in the pathogenesis of keratoacanthoma. The most enriched canonical pathways were clathrin-mediated endocytosis signaling, molecular mechanisms of cancer and integrin signaling. The distinctive gene expression profile of keratoacanthoma reveals that it is molecularly distinct from squamous cell carcinoma. The molecular pathways and genes differentially expressed in comparing keratoacanthoma with normal skin suggest that keratoacanthoma is a neoplasm that can regress due to upregulation of the cell death/apoptosis pathway.

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“…55 Moreover, higher levels of ESE-1 mRNA and protein expression were detected in breast cancer cells than in normal breast epithelial cells, and an overexpression of ESE-1 has been observed in primary breast tumor specimens as compared to normal mammary tissues. 56 ESE-1 expression is also upregulated in a subset of breast tumors and breast cancer-derived cell lines that express high levels of the Her2/Neu proto-oncogene, which is also known as erythroblastic leukemia viral oncogene homolog 2 (ErbB-2). 54 Transient reporter assays using the ESE-1 promoter have shown that ESE-1 transcription is regulated by ErbB-2 receptor signaling in epithelial breast cancer cells, where expression of ErbB-2 upregulates ESE-1 promoter activity, while inhibition of ErbB-2 or its downstream signaling pathways decrease both ESE-1 promoter activity and endogenous ESE-1 protein levels.…”

Section: Ese-1 In Mammary Gland Development and Breast Cancermentioning

confidence: 99%

Multiple roles of the epithelium-specific ETS transcription factor, ESE-1, in development and disease

Oliver

Kushwah

2012

Laboratory Investigation

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The E26 transformation-specific (ETS) family of transcription factors comprises of 27 and 26 members in humans and mice, respectively, which are known to regulate many different biological processes, including cell proliferation, cell differentiation, embryonic development, neoplasia, hematopoiesis, angiogenesis, and inflammation. The epitheliumspecific ETS transcription factor-1 (ESE-1) is a physiologically important ETS transcription factor, which has been shown to play a role in the pathogenesis of various diseases, and was originally characterized as having an epithelial-restricted expression pattern, thus placing it within the epithelium-specific ETS subfamily. Despite a large body of published work on ETS biology, much remains to be learned about the precise functions of ESE-1 and other epithelium-specific ETS factors in regulating diverse disease processes. Clues as to the specific function of ESE-1 in the setting of various diseases can be obtained from studies aimed at examining the expression of putative target genes regulated by ESE-1. Thus, this review will focus primarily on the various roles of ESE-1 in different pathophysiological processes, including regulation of epithelial cell differentiation during both intestinal development and lung regeneration; regulation of dendritic cell-driven T-cell differentiation during allergic airway inflammation; regulation of mammary gland development and breast cancer; and regulation of the effects of inflammatory stimuli within the setting of synovial joint and vascular inflammation. Understanding the exact mechanisms by which ESE-1 regulates these processes can have important implications for the treatment of a wide range of diseases. KEYWORDS: cancer; development; epithelial cell differentiation; ESE-1; ETS transcription factor; inflammation; regeneration The E26 transformation-specific (ETS) family of transcription factors is characterized by a highly conserved 84-aminoacid DNA-binding domain, known as the ETS domain. 1 Because the first member of the ETS family, the v-ets oncogene, was originally discovered as part of a fusion protein with gag and myb expressed by the E26 avian erythroblastosis-transforming retrovirus and its DNA-binding domain is E26 transformation-specific, this 84-amino-acid DNA-binding domain was named the ETS domain. 1 The ETS domain is usually located within the carboxyl-terminal region of the protein as a winged helix-turn-helix structural motif and mediates binding to sites of purine-rich DNA, commonly containing a core consensus sequence of GGAA/T, within the promoter and enhancer regions of target genes. 2,3 Many ETS transcription factors also contain a pointed domain, which is located within the amino-terminal region and is involved in protein-protein interactions. 2 Approximately 30 members of the ETS transcription factor family have been identified in mammals (ie 27 in humans and 26 in mice), 4 and have been shown to play crucial roles in the regulation of many physiological and pathological processes, such as embryonic develo...

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Profile of Ets gene expression in human breast carcinoma

Cited by 49 publications

References 34 publications

Expression characteristics of prostate-derived Ets factor support a role in breast and prostate cancer progression

Expression characteristics of prostate-derived Ets factor support a role in breast and prostate cancer progression

Keratoacanthoma and squamous cell carcinoma are distinct from a molecular perspective

Multiple roles of the epithelium-specific ETS transcription factor, ESE-1, in development and disease

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