Profile analysis of <i>C. elegans</i> rheotaxis behavior using a microfluidic device

Ge, Anle; Wang, Xixian; Ge, Minghai; Hu, Liang; Feng, Xiaojun; Du, Wei; Liu, Bi‐Feng

doi:10.1039/c8lc01087k

Cited by 17 publications

(7 citation statements)

References 39 publications

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“…elegans [159] / DNA [160] /B. subtilis [81] Shear rate [82] /mechanical forces [159] / ASH neurons [161] /superficial neuromasts [162] Redox-taxis Redox gradient E. coli [51] /A. brasilense [163] Telotaxis Visual fixation View sight Thermotaxis Temperature gradient Spermatozoa [71] /C.…”

Section: Focal Adhesionmentioning

confidence: 99%

Collective Behaviors of Active Matter Learning from Natural Taxes Across Scales

Pumera

et al. 2022

Advanced Materials

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Figure 1. Cognate collective taxis behaviors realized by organisms and robots across scales. A) Collective migration of particle robots and cells. Loosely coupled particles present collective phototaxis (left). Reproduced with permission. [11] Copyright 2019, Springer Nature. Cells perform collective migration in 3D environments (right). Reproduced with permission. [4] Copyright 2018, Springer Nature. B) Self-assembly of robots and bacteria. A swarm of 1024 kilobots form a star-like pattern (left). Reproduced with permission. [14] Copyright 2014, AAAS. A swarm of E. coli cells form a right-handed colony (right). Scale bar: 5 mm. Reproduced with permission. [15] Copyright 2011, The Proceedings of the National Academy of Sciences. C) Collective taxes of macroscopic organisms (e.g., fishes, birds, and ants) and robots. D) Collective taxes of microorganisms (e.g., sperms, [19] cells, and bacteria [20] ) and microrobots. [21] E) Connection constructed by ants and NPs. Weaver ants establish a bridge to connect leaves (left). Reproduced with permission. [7] Copyright 2002, Springer Nature. Magnetic NPs with gold functionalization connect two broken electrodes in a targeted position (right). Reproduced with permission. [16] Copyright 2019, American Chemical Society. F) Avoidance of predators. A bait ball of fishes is formed during the hunting of a sea lion (left). Reproduced with permission. [22] Copyright 2019, Science and Information Organization. The biomimetic predator-prey behavior in a binary system of active micromotors (right). Reproduced with permission. [17] Copyright 2019, American Chemical Society.

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Section: Focal Adhesionmentioning

confidence: 99%

Collective Behaviors of Active Matter Learning from Natural Taxes Across Scales

Pumera

et al. 2022

Advanced Materials

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“…This prevented the use of unidirectional pulses because approximately half of the worms would have been exposed to cathodal pulses, leading to no egg-laying response and waste of animals. Similar longitudinal orientation in the multi-worm device may have been achieved by preconditioning the worms with a longitudinal stimulus (e.g., controlled EF 37 or flow 38 ), but this would have added complexity to our procedures.…”

Section: Assay Time and Ef Pulsation Effectsmentioning

confidence: 99%

Microfluidic Electric Egg-Laying Assay and Application to In-vivo Toxicity Screening of Microplastics using C. elegans

Youssef

Archonta

Kubiseski

et al. 2021

Preprint

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Environmental pollutants like microplastics are posing health concerns on aquatic animals and the ecosystem. Microplastic toxicity studies using C. elegans as a model are evolving but methodologically hindered from obtaining statistically strong data sets, detecting toxicity effects based on microplastics uptake, and correlating physiological and behavioural effects at an individual-worm level. In this paper, we report a novel microfluidic electric egg-laying assay for phenotypical assessment of multiple worms in parallel. The effects of glucose and polystyrene microplastics at various concentrations on the worms’ electric egg-laying, length, diameter, and length contraction during exposure to electric signal were studied. The device contained eight parallel worm-dwelling microchannels called electric traps, with equivalent electrical fields, in which the worms were electrically stimulated for egg deposition and fluorescently imaged for assessment of neuronal and microplastic uptake expression. A new bidirectional stimulation technique was developed, and the device design was optimized to achieve a testing efficiency of 91.25%. Exposure of worms to 100mM glucose resulted in a significant reduction in their egg-laying and size. The effects of 1μm polystyrene microparticles at concentrations of 100 and 1000 mg/L on the electric egg-laying behaviour, size, and neurodegeneration of N2 and NW1229 (expressing GFP pan-neuronally) worms were also studied. Of the two concentrations, 1000 mg/L caused severe egg-laying deficiency and growth retardation as well as neurodegeneration. Additionally, using single-worm level phenotyping, we noticed intra-population variability in microplastics uptake and correlation with the above physiological and behavioural phenotypes, which was hidden in the population-averaged results. Taken together, these results suggest the appropriateness of our microfluidic assay for toxicological studies and for assessing the phenotypical heterogeneity in response to microplastics.

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“…The microfluidic device was fabricated using soft lithography. , The fluidic mold was produced via ultraviolet patterning of the SU-8 photoresist on a silicon wafer. To fabricate the PDMS device, the fluidic mold was exposed to trimethylchlorosilane vapor for 3 min.…”

Section: Experimental Sectionmentioning

confidence: 99%

Comprehensive Evaluation of Stable Neuronal Cell Adhesion and Culture on One-Step Modified Polydimethylsiloxane Using Functionalized Pluronic

et al. 2020

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Polydimethylsiloxane (PDMS) is a popular and property-advantageous material for developing biomedical microsystems and advancing cell microengineering. The requirement of constructing a robust cell-adhesive PDMS interface drives the exploration of simple, straightforward, and applicable surface modification methods. Here, a comprehensive evaluation of highly stable neuronal cell adhesion and culture on the PDMS surface modified in one step using functionalized Pluronic is presented. According to multiple comparative tests, this modification is sufficiently verified to enable more significant cell adhesion and spreading in both quantity and stability, higher neuronal differentiation and viability/growth, more complete formation of the neuronal network, and stabler neuronal cell culture than the common coating tools on the PDMS substrate. The comparable and even superior cellular effects of this modification on PDMS to the standard coating of polystyrene for in vitro neurological research are demonstrated. Long-term microfluidic neuron culture with stable adhesion and high differentiation on the modified PDMS interface is accomplished, too. The achievement provides a detailed experimental demonstration of this simple and effective modification for strengthening neuronal cell culture on the PDMS substrate, which is useful for potential applications in the fields of neurobiology, neuron microengineering, and brain-on-a-chip.

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Profile analysis of C. elegans rheotaxis behavior using a microfluidic device

Abstract: We report a microfluidic device for the analysis of the rheotaxis behavior of C. elegans.

Cited by 17 publications

References 39 publications

Collective Behaviors of Active Matter Learning from Natural Taxes Across Scales

Collective Behaviors of Active Matter Learning from Natural Taxes Across Scales

Microfluidic Electric Egg-Laying Assay and Application to In-vivo Toxicity Screening of Microplastics using C. elegans

Comprehensive Evaluation of Stable Neuronal Cell Adhesion and Culture on One-Step Modified Polydimethylsiloxane Using Functionalized Pluronic

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