Profil bactériologique des bactériémies à germes multirésistants à l'hôpital Charles-Nicolle de Tunis

Saïdani, Messaoud; Boutiba, Ilhem; Ghozzi, Rafiaa; Kammoun, A.; Redjeb, S. Ben

doi:10.1016/j.medmal.2005.06.010

Cited by 8 publications

(6 citation statements)

References 4 publications

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“…21/34), followed by MDR P. aeruginosa (26.5%, 9/34) and MRSA (11.7%, 4/34). Saidani et al, (29) had previously reported a predominance of ESBL-producing Enterobacteriaceae in their study. In our study, ESBL-E expressed high resistance to aminoglycosides with 66.7% to gentamicin, 61.9% to tobramycin and 47.6% to amikacin.…”

Section: Antibiotic Profile Of Selected Mdr Bacteriamentioning

confidence: 70%

Profile of multidrug-resistant clinical bacterial isolates at the National Hospital of Zinder (NHZ), Niger Republic in 2021

Abdoulaye¹,

Bacha²,

Aghali³

et al. 2022

Af J Clin Exp Micro

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Background: Today, bacterial resistance is a public health challenge throughout the world, and infections caused by resistant bacteria are associated with increased morbidity, mortality and health care costs. The objective of this descriptive study is to determine the prevalence and distribution of multi-drug resistant (MDR) clinical bacteria isolates at the National Hospital of Zinder, Niger Republic in 2021.Methodology: We conducted a descriptive cross-sectional study of in- and out-patients from whose clinical samples’ bacteria were isolated at the bacteriology unit of the laboratory. Bacteria were isolated from the clinical samples following standard aerobic cultures and identified using conventional biochemical test schemes. Antibiotic susceptibility testing (AST) was performed by the agar disk diffusion technique, and categorization of the isolates into sensitive, intermediate or resistant was done according to the recommendations of the Antibiogram Committee of the French Society of Microbiology (CA-SFM) 2020 version 1.2. MDR was defined as resistance to at least one antibiotic in three or more categories, while selected MDR bacteria such as ESBL was identified using double disk synergy test, and MRSA by cefoxitin disk diffusion test.Results: Seventy-seven (6.7%) bacterial species were isolated from 1153 clinical samples processed at the bacteriology unit of the hospital laboratory between June and December 2021, of which 65.0% (50/77) were members of the order Enterobacteriales. Escherichia coli represented 40.3% (40/77) of the isolated bacteria, Staphylococcus aureus 13.0% (10/77) and Pseudomonas aeruginosa 11.7% (9/77). The overall prevalence of MDR was 44.2% (34/77), including 61.8% (21/34) ESBL-producing Enterobacteriales (ESBL-E), 26.5% (9/34) multi-resistant P. aeruginosa and 11.7% (4/34) MRSA, with 67.6% (23/34) of the MDR isolates from outpatients. Resistance rates of the Enterobacteriales to ciprofloxacin, gentamicin, amikacin and imipenem were 62.0%, 52.0%, 38.0% and 8.0% respectively. Resistance rates of P. aeruginosa were 100.0%, 88.9%, 77.8%, 33.3%, 22.2%, and 22.2% respectively to ceftazidime, ticarcillin, imipenem, ciprofloxacin, levofloxacin, and amikacin. Resistance rates of S. aureus were 100.0%, 50.0%, 40.0%, 10.0%, 0% and 0% to penicillin G, erythromycin, cefoxitin, tetracycline, fusidic acid, and chloramphenicol respectively. ESBL-E were 47.6%, 85.7% and 0% resistant to amikacin, ciprofloxacin and imipenem, and MRSA resistance rates were 75.0%, 75.0%, 50.0% and 0% to erythromycin, tetracycline, gentamicin, and chloramphenicol respectively.Conclusion: This study reports high prevalence of MDR bacteria, mainly ESBL-E, with concerning high resistance to carbapenem. Rational use of antibiotics and implementation of surveillance system for MDR bacteria must be implemented in order to limit the emergence and spread of MDR bacteria in Niger Republic. Contexte: Aujourd'hui, la résistance bactérienne est un défi de santé publique dans le monde entier, et les infections causées par des bactéries résistantes sont associées à une augmentation de la morbidité, de la mortalité et des coûts des soins de santé. L'objectif de cette étude descriptive est de déterminer la prévalence et la distribution des isolats cliniques de bactéries multirésistantes (MDR) à l'Hôpital National de Zinder,République du Niger en 2021.Méthodologie: Nous avons mené une étude transversale descriptive des patients hospitalisés et ambulatoires dont les bactéries des échantillons cliniques ont été isolées à l'unité de bactériologie du laboratoire. Les bactéries ont été isolées des échantillons cliniques à la suite de cultures aérobies standard et identifiées à l'aide de schémas de tests biochimiques conventionnels. Les antibiogrammes (AST) ont été réalisés par la technique de diffusion sur disque de gélose, et la catégorisation des isolats en sensibles, intermédiaires ou résistants a été faite selon les recommanda- tions du Comité Antibiogramme de la Société Française de Microbiologie (CA-SFM) 2020 version 1.2. La MDR a été définie comme la résistance à au moins un antibiotique dans trois catégories ou plus, tandis que certaines bactéries MDR telles que les BLSE ont été identifiées à l'aide d'un test de synergie à double disque et le SARM par le test de diffusion sur disque de céfoxitine.Résultats: Soixante-dix-sept (6,7%) espèces bactériennes ont été isolées à partir de 1153 échantillons cliniques traités à l'unité de bactériologie du laboratoire hospitalier entre juin et décembre 2021, dont 65,0% (50/77) appartenaient à l'ordre des Enterobacteriales. Escherichia coli représentait 40,3% (40/77) des bactéries isolées, Staphylococcus aureus 13,0% (10/77) et Pseudomonas aeruginosa 11,7% (9/77). La prévalence globale des MDR était de 44,2% (34/77), dont 61,8% (21/34) d'Enterobacteriales productrices de BLSE (BLSE-E), 26,5% (9/34) de P. aeruginosa multirésistantes et 11,7% (4/34) SARM, avec 67,6% (23/34) des isolats de MDR provenant de patients externes. Les taux de résistance des Enterobacteriales à la ciprofloxacine, à la gentamicine, à l'amikacine et à l'imipénème étaient respectivement de 62,0%, 52,0%, 38,0% et 8,0%. Les taux de résistance de P. aeruginosa étaient respectivement de 100,0%, 88,9%, 77,8%,33,3%, 22,2% et 22,2% à la ceftazidime, à la ticarcilline, à l'imipénème, à la ciprofloxacine, à la lévofloxacine et à l'amikacine. Les taux de résistance de S. aureus étaient respectivement de 100,0%, 50,0%, 40,0%, 10,0%, 0% et 0% à la pénicilline G, à l'érythromycine, à la céfoxitine, à la tétracycline, à l'acide fusidique et au chloramphénicol. Les BLSE-E étaient de 47,6%, 85,7% et 0% de résistance à l'amikacine, à la ciprofloxacine et à l'imipénème, et les taux de résistance au SARM étaient respectivement de 75,0%, 75,0%, 50,0% et 0% à l'érythromycine, la tétracycline, la gentamicine et le chloramphénicol. Conclusion: Cette étude rapporte une prévalence élevée de bactéries MDR, principalement des BLSE-E, avec une résistance élevée aux carbapénèmes. L'utilisation rationnelle des antibiotiques et la mise en place d'un système de surveillance des bactéries MDR doivent être mises en oeuvre afin de limiter l'émergence et la propagation des bactéries MDR en République du Niger.

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Section: Antibiotic Profile Of Selected Mdr Bacteriamentioning

confidence: 70%

Profile of multidrug-resistant clinical bacterial isolates at the National Hospital of Zinder (NHZ), Niger Republic in 2021

Abdoulaye¹,

Bacha²,

Aghali³

et al. 2022

Af J Clin Exp Micro

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“…[ 3 ] The mec gene complex is a complex regulatory system with 2 repressor/anti-repressor systems of mecI/mecR1 and blaI/blaR. [ 15 , 16 ] If the mecI/mecR1 system is functional, transcription of the mecA gene is strongly inhibited and the strain appears sensitive, with the usual antibiogram techniques, however, thanks to the blaI/blaR1 system, methicillin will slowly induce resistance. [ 17 ]…”

Section: Discussionmentioning

confidence: 99%

Prevalence and molecular detection of Staphylococcus aureus resistance to antibiotics

Dendi,

Allem,

Sebaihia

et al. 2024

Medicine

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In Algeria, the issue of antibiotic resistance is on the rise, being the Staphylococcus aureus infection as a significant concern of hospital-acquired infections. The emergence of antibiotic resistance in this bacterium poses a worldwide challenge. The aim of this study aims to establish the incidence of S aureus strains in Algeria as well as identify phenotypic and genotypic resistance based on the “mecA” and “nuc” genes. From 2014 to 2017, a total of 185 S aureus strains were isolated from patients at a hospital in the city of Rouïba, Algiers the number of isolates was slightly higher in males at 58.06% compared to females at 41.94%, resulting in a sex ratio of 1.38. the Oxacillin and Cefoxitin DD test (1 μg oxacillin disk and 30 μg cefoxitin disk) identified 42 strains as resistant. The results indicated high resistance to lactam antibiotics, with penicillin having a 100% resistance rate. There was also significant resistance to oxacillin (51.25%) and cefoxitin (50%). This resistance was frequently associated with resistance to other antibiotic classes, such as aminoglycosides (50%) and Macrolides (28.29%). To confirm methicillin-resistant characteristics, a polymerase chain reaction (PCR) multiplex was conducted on 10 isolates (6 SARM; 4 MSSA) on a phenotypic level. Three isolates tested positive for “mecA,” while 7 were negative. All strains carry the nuc gene, which is specific to S aureus. In Algeria, the incidence of S aureus resistance is slightly lower compared to other countries, but it is increasing over time. It is now more crucial than ever to restrict the proliferation of multidrug-resistant strains and reduce undue antibiotic prescriptions. To achieve this, it is vital to keep updated on the epidemiology of this bacterium and its antibiotic susceptibility. This will enable the formulation of appropriate preventive control measures to manage its progression.

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“…and Escherichia coli) were the most resistant bacteria to antibiotics and showed resistance to the majority of the antibiotics tested. The rate of resistance to third generation cephalosporins and other βlactam antibiotics can be explained by the high production of penicillinase and the production of extended spectrum β-lactamase (Saïdani et al 2006). However, the rate of this resistance is high compared to those reported in developed countries (WHO, 2021).…”

Section: Discussionmentioning

confidence: 99%

Resistance profile of pathogenic strains isolated from community acquired infection at the Protestant Hospital of Ngaoundere (Cameroon)

Talom,

Dahara,

Nodem

et al. 2024

Int. J. Bio. Chem. Sci

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Worldwide data show that there is increasing resistance among infection bacteria to conventional drugs. The present study aimed at determining data on susceptibility of bacteria isolated from patient for community acquired infection in Protestant Hospital of Ngaoundere against prescribed antibiotics in case of infections by these bacteria. Bacteriological profile of collected samples (stool, urine, cervico-vaginal and urethral secretion, blood) was carry out by standard method. Antibiotic susceptibility was done by the agar disk diffusion method. A total of 120 samples were collected. Among 46 samples was positive for bacterial infections germs (38% of prevalence). Stomach pain represents the most frequent complaint of patients (33.1%). Enterobacteriaceae were the most isolated (70%) followed by Neisseria gonorrhoeae (15%). About 73.0% of Enterobacteriaceae identified were resistant to ticarcillin while 80% of N. gonorrhoeae to ampicillin. Some of the isolates were resistant to ticarpen (73%), oxacillin (71%) and tobramycin (57%) which are more frequently prescribed and indicates that increased consumption of a particular antibiotic leads to acquisition of resistance. Enterobacteriaceae were predominant bacterial isolated and it is necessary to continue monitoring the consumption of antibiotics to ensure good management of infections.

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Profil bactériologique des bactériémies à germes multirésistants à l'hôpital Charles-Nicolle de Tunis

Cited by 8 publications

References 4 publications

Profile of multidrug-resistant clinical bacterial isolates at the National Hospital of Zinder (NHZ), Niger Republic in 2021

Profile of multidrug-resistant clinical bacterial isolates at the National Hospital of Zinder (NHZ), Niger Republic in 2021

Prevalence and molecular detection of Staphylococcus aureus resistance to antibiotics

Resistance profile of pathogenic strains isolated from community acquired infection at the Protestant Hospital of Ngaoundere (Cameroon)

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