Proficiencies of Three Anaerobic Culture Systems for Recovering Periodontal Pathogenic Bacteria

Abstract

Anaerobic culture is employed routinely in the primary isolation of periodontal pathogenic bacteria. However, little or no data exist on the relative abilities of the Coy anaerobic chamber (Coy Laboratory Products, Grass Lake, Mich.), the GasPak (Becton Dickinson Microbiology Systems, Cockeysville, Md.), and the AnaeroPack (Mitsubishi Gas Chemical America, Inc., New York, N.Y.) systems to grow important periodontal species, including Porphyromonas gingivalis,Prevotella intermedia/nigrescens, Bacteroides forsythus, Eubacterium species,Campylobacter species, Fusobacterium species, and Peptostreptococcus micros. A total of 78 specimens from advanced periodontitis lesions were collected anaerobically, plated on enriched blood agar medium, and incubated at 35°C for 5 to 7 days in each anaerobic culture system. The three culture systems were equally efficient in isolating Porphyromonas gingivalis andPrevotella intermedia/nigrescens. The Coy anaerobic chamber yielded the highest proportional recoveries ofCampylobacter (P = 0.0001; nonparametric analysis of variance) and Eubacterium (P = 0.009). The Coy anaerobic chamber and the GasPak system demonstrated higher proportional recoveries of Bacteroides forsythus(P = 0.0006) and Peptostreptococcus micros(P = 0.0001) than the AnaeroPack system. The AnaeroPack system was most efficient in growingFusobacterium species (P = 0.0001). Overall, the Coy anaerobic chamber and the GasPak system showed the highest proportional recoveries of putative periodontal pathogens, but the recoveries by the various anaerobic test systems varied considerably from sample to sample.

Periodontopathogen and Epstein‐Barr Virus Contamination Affects Transplanted Bone Volume in Sinus Augmentation

“…Samples from the anaerobic transport media were processed for culture in conventional selective and non‐selective media 30‐32 within 24 hours of collection for the detection of specific periodontopathogens: Aa , Pg , Pi , Tannerella forsythia (Tf) (previously Tannerella forsythensis ), Treponema denticola (Td) , Dialister pneumosintes (Dp) , Fusobacterium spp., Campylobacter spp., Parvimonas micra (Pm) , Eikenella corrodens (Ec) , and enteric Gram‐negative rods (Er).…”

Porphyromonas gingivalis:patógeno predominante en la periodontitis crónica

“…34,35 Siendo el más utilizado el cono de papel número 30 ó 40, que se colocan dentro del surco o bolsa periodontal, por un periodo de 20 a 60 seg, para luego ser llevado a un medio de transporte como VMGA-III, 36 BHI, Tioglicolato, y luego ser sembrado en medios enriquecidos como Agar sangre suplementado o el medio selectivo Agar Columbia antibiótico 37 e incubar a 37 ºC por 7 a 14 días, en condiciones de anaerobiosis, que puede ser por Jarra, cámara o sobres de anaerobiosis. 38 Pasado el tiempo, la lectura de las colonias debe reconocer características como; tamaño de 1-2 mm, forma redonda, convexa y ser pigmentados de un color marrón a negro. La coloración gram debe evidenciar una morfología cocobacilar de 0.5x1-2 um y ser negativa.…”

Toothbrush contamination in family members

“…Each participant received 1 toothbrush (Colgate® Twister, Colgate® junior) and a tube of toothpaste (MFP-Colgate®) for home use and was instructed to return the toothbrush after 1 month to the investigators. Subgingival microbiota and toothbrush contamination were analyzed using microbial culture techniques for the presence of periodontopathic bacteria according to Doan et al (15) and Slots (16) . Briefly, most samples were processed before 24 hours with a maximum of 48 hours at room atmosphere (25ºC) and immediately incubated in CO 2 and anaerobic culture systems.…”