Proficiencies of Artemisia scoparia against CCl4 induced DNA damages and renal toxicity in rat

Sajid, M.; Khan, Muhammad Rashid; Shah, Naseer Ali; Ullah, Shafi; Majid, Muhammad; Ahmad, Bushra; Nigussie, Dereje

doi:10.1186/s12906-016-1137-6

Cited by 39 publications

(27 citation statements)

References 29 publications

(34 reference statements)

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“…Such injuries might augment the hepatic injuries and functional anomalies. The results recorded in this study were in conformity to Sajid et al [61] where the comet length had increased while the %DNA in head was decreased with CCl 4 treatment to rats. However a restoration in these comet parameters was recorded with co-administration of methanol extract of Artemisia scoparia [61].…”

Section: Discussionsupporting

confidence: 92%

Protective effects of Fraxinus xanthoxyloides (Wall.) leaves against CCl4 induced hepatic toxicity in rat

Khan

Sajid

2016

BMC Complement Altern Med

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BackgroundLeaves and root bark of Fraxinus xanthoxyloides Wall. (Oleaceae) are used locally for the treatment of jaundice, malaria and pneumonia. Decoction of stem, twigs and bark is used in pain, internal injuries, rheumatism and in bone fracture. In this investigation we have evaluated the methanol extract of leaves for its hepatoprotective potential against CCl4 induced hepatic injuries in rat.MethodsPowder of F. xanthoxyloides leaves was extracted with methanol (FXM) and subjected for the determination of polyphenolics through HPLC-DAD analysis. Sprague–Dawley (Rattus novergicus) male rats were divided into eight groups (six rats in each). Group I: non-treated control; Group II: vehicle treated (DMSO plus olive oil) while Group III- VI treated with 1 ml/kg body weight (bw) of CCl4 (30 % in olive oil) for 30 days (15 dosages) to induce the hepatic injuries. Group IV: treated with silymarin (100 mg/kg bw); Group V and VI with FXM (200, 400 mg/kg bw) on alternate days with CCl4 treatment. Group VII and VIII was administered with FXM (200, 400 mg/kg bw) alone (15 dosages). After 30 days the serum was evaluated for liver function enzymes and biochemical markers, liver samples for antioxidant enzymes, biochemical markers, comet assay and for histopathology.ResultsHPLC-DAD analysis of FXM revealed the existence of rutin and caffeic acid. In CCl4 treated rats the level of alanine transaminase (ALT), aspartate transaminase (AST), total bilirubin was significantly increased while the albumin concentration in serum was decreased as compared to control group. The level of hepatic antioxidant enzymes, catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione-S-transferase (GST) and glutathione reductase (GSR) was significantly decreased against the control group. Further, significant decrease in GSH while increase in lipid peroxides (TBARS), H2O2, DNA damages and comet length was induced with CCl4 in hepatic tissues of rat. In contrast, co-administration of FXM and silymarin restored the biochemical and histopathological status of the liver.ConclusionResults of present investigation revealed that F. xanthoxyloides leaves possibly protect the liver against CCl4 induced injuries like silymarin by its antioxidant constituents.

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Section: Discussionsupporting

confidence: 92%

Protective effects of Fraxinus xanthoxyloides (Wall.) leaves against CCl4 induced hepatic toxicity in rat

Khan

Sajid

2016

BMC Complement Altern Med

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“…Free radicals can induce abnormal proteins, depletion of antioxidants, immune system and changes in gene expression [3]. The antioxidants such as polyphenolics can protect the biological systems against the harmful effects of oxidative processes [4]. Thus, increased consumption of plant derived products which are good sources of antioxidants is a convenient way to control the diseases induced with oxidative stress [5].…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat

Rashid¹,

Khan

Sajid

2016

BMC Complement Altern Med

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Background Fagonia olivieri (DC) being used for the treatment of diabetes, cancer, fever and claimed to be effective in many other stress related disorders. In this study we have evaluated the F. olivieri whole methanol extract and its derived fractions for various in vitro and in vivo antioxidant studies.MethodsThe crude methanol extract of the whole plant of F. olivieri (FOM) and its derived fractions; n-hexane (FOH), chloroform (FOC), ethyl acetate (FOE), n-butanol (FOB) and aqueous (FOA) were evaluated for the total phenolic and flavonoid content and in vitro antioxidant abilities. The antioxidant effect of FOM was determined by acetaminophen-induced hepatotoxicity in Sprague–Dawley (Rattus novergicus) male rats. The methanol/fractions were also analysed by HPLC analysis for the presence of polyphenolics.ResultsThe total phenolic content of the samples ranged from 19.3 ± 0.529 to 106.2 ± 0.892 mg GAE/g extract while total flavonoid content 16.2 ± 0.881 to 50.1 ± 1.764 mg RTE/g extract, respectively. FOA showed highest radical scavenging activity for DPPH (IC50 = 55.2 ± 1.212 μg/ml), ABTS (IC50 = 90.2 ± 1.232 μg/ml) superoxide (IC50 = 37.1 ± 0.643 μg/ml) and for H2O2 (IC50 = 64 ± 1.463 μg/ml). FOE exhibited the highest antioxidant activities for phosphomolybdenum (IC50 = 78.2 ± 0.883 μg/ml) and for hydroxyl radical scavenging (IC50 = 82 ± 2.603 μg/ml). HPLC analysis of FOM and its derived fractions showed the presence of rutin, catechin and gallic acid. Elevated levels of AST, ALT, ALP, LDH and lipid profile in serum and lipid peroxidation and DNA damages in liver; while decreased activity level of CAT, SOD, GSH-Px, GR and reduced glutathione (GSH) concentration induced with acetaminophen in rat were reverted towards the control group with co-administration of FOM.ConclusionOur results showed that F. olivieri is a potential source of natural antioxidants, which justifies its use in folklore medicine.

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“…Although the polyphenols demonstrate hepatoprotection due to their antioxidant effect, there are other effects such as immunomodulatory and antiinflammatory 28 . Different in vivo studies on animals reported that carbon tetrachloride causes liver and kidney injuries 29,30 through generation of electrophilic trichloromethyl (CCl3) radicals 31 . Similar hepatoprotective effects of different seed extracts of red grape varieties were reported not only in the model of CCl4 induced liver damage in rats 32 , but also in the experiments that involed the poisoning of rats with ethanol 33,34 , acetaminophen-induced liver injury 35,36 , dimethylnitrosamine-induced liver damage in rats 35,36 , as well as on liver steatosis 37 and in γ-irradiated rat 38 .…”

Section: Histopathology Of Kidneymentioning

confidence: 99%

Hepato- and Nephroprotective Effects of the Polyphenol Concentrate From Cabernet Sauvignon Grape Varieties Cultivated in Kazakhstan in the Experimental Model Of CCL4-Induced Toxicity

Nurgozhin

Sergazy

Adilgozhina

et al. 2017

IJTPR

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Objective:This study investigates the hepatoprotective effect and the antioxidant role of polyphenol concentrate in the experimental model of carbon tetrachloride (CCl4) induced toxicity. Methods: Antioxidant activity of Cabernet Sauvignon grape polyphenol were evaluated by radical scavenging of 1,1-diphenyl-2-picryl hydrazyl radical (DPPH), 2,2’-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS.+). In addition, the effects of polyphenol concentrate on the survival of Wistar rats in the toxicity model, was also investigated. The polyphenol concentrate was administered for 5 five days prior to injection of carbon tetrachloride in a sub-lethal dose of 300 mg/kg of animal body weight in order to perform histological examinations of the liver and kidney, and detect the levels of AST, ALT and bilirubin. Results: Administration of polyphenol concentrate increased animal survival in the experimental model. Moreover, the intragastric administration of polyphenol concentrate prior to the initiation of the experimental model of toxicity, which was caused by a sub-lethal CCl4 dose, reduced morphological injuries in the liver and kidney, decreased the AST and ALT levels of the blood serum. Discussion and conclusion: Our data demonstrate that polyphenol concentrate possesses an antioxidant potential both in vitro and in vivo by reducing antioxidant stress that was caused by CCl4 administration into rats.

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Proficiencies of Artemisia scoparia against CCl4 induced DNA damages and renal toxicity in rat

Cited by 39 publications

References 29 publications

Protective effects of Fraxinus xanthoxyloides (Wall.) leaves against CCl4 induced hepatic toxicity in rat

Protective effects of Fraxinus xanthoxyloides (Wall.) leaves against CCl4 induced hepatic toxicity in rat

Hepatoprotective potential of Fagonia olivieri DC. against acetaminophen induced toxicity in rat

Hepato- and Nephroprotective Effects of the Polyphenol Concentrate From Cabernet Sauvignon Grape Varieties Cultivated in Kazakhstan in the Experimental Model Of CCL4-Induced Toxicity

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