Profenofos insecticide degradation by novel microbial consortium and isolates enriched from contaminated chili farm soil

Siripattanakul‐Ratpukdi, Sumana; Vangnai, Alisa S.; Sangthean, P.; Singkibut, Sirinuttakan

doi:10.1007/s11356-014-3354-1

Cited by 37 publications

(24 citation statements)

References 18 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus. this organism was versatile in degrading organophosphate pesticides at higher initial concentration than compared to other organisms . The organism also utilized various halogenated aromatic compounds as shown in Table .…”

Section: Resultsmentioning

confidence: 99%

“…Bioremediation is an efficient, eco‐friendly, and economical method for the decontaminated of polluted ecosystems. Profenofos has been reported to be degraded by Pseudomonas putida and Burkholderia gladioli [12], Pseudomonas aeruginosa , Bacillus subtilis , and Pseudomonas species . These organisms were shown to degrade profenofos by hydrolysis to yield 4‐bromo‐2‐chlorophenol as metabolite but its complete degradative pathway has not been studied.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biodegradation of pesticide profenofos by the free and immobilized cells ofPseudoxanthomonas suwonensisstrain HNM

Talwar

Ninnekar

2015

J. Basic Microbiol.

View full text Add to dashboard Cite

Profenofos is an organophosphate pesticide used extensively in agriculture to control pests. A bacterium capable of degrading profenofos was isolated from pesticide-contaminated soil samples and identified as Pseudoxanthomonas suwonensis strain HNM based on its morphological and biochemical characteristics and phylogenetic analysis of 16S rRNA gene sequences. 4-Bromo-2-chlorophenol was identified as a metabolite of profenofos degradation by HPLC and GC-MS analysis. The organism degraded profenofos by hydrolysis to yield 4-bromo-2-chlorophenol which was further utilized as carbon source for growth. The organism utilized various organophosphate pesticides such as temephos, quinalphos, and chloropyrifos as carbon sources. The optimum conditions for degradation of profenofos by P. suwonensis strain HMN were found to be at pH 7 and 30 °C. We have investigated the rate of degradation of profenofos by the free and immobilized cells of P. suwonensis strain HNM in various matrices such as sodium alginate (SA), sodium alginate-polyvinyl alcohol (SA-PVA), and SA-bentonite clay. The rate of degradation of 3 and 6 mM profenofos by the freely suspended cells were compared with that by immobilized cells in batches and semi-continuous with shaken cultures. The SA-bentonite clay-immobilized cells showed higher rate of degradation of 3 and 6 mM profenofos then freely suspended cells and cells immobilized in SA and SA-PVA. The SA-bentonite clay-immobilized cells of P. suwonensis strain HNM could be reused for more than 32 cycles without losing their degradation capacity. Thus, the immobilized cells are more efficient than freely suspended cells for the degradation of organophosphate pesticide contaminated water.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biodegradation of pesticide profenofos by the free and immobilized cells ofPseudoxanthomonas suwonensisstrain HNM

Talwar

Ninnekar

2015

J. Basic Microbiol.

View full text Add to dashboard Cite

show abstract

“…Many organophosphate pesticide-degrading bacteria have been reported in recent years [ 48 , 56 , 57 ]. Moreover, many Citrobacter species can degrade phenols and phosphates [ 58 – 60 ], and their degradation pathways are widely recognized.…”

Section: Discussionmentioning

confidence: 99%

Gut symbiont enhances insecticide resistance in a significant pest, the oriental fruit fly Bactrocera dorsalis (Hendel)

et al. 2017

View full text Add to dashboard Cite

BackgroundSymbiotic bacteria affect insect physiology and ecology. They may also mediate insecticide resistance within their hosts and thereby impact pest and vector control practices. Here, we document a novel mechanism of insecticide resistance in which a gut symbiont of the tephritid pest fruit fly Bactrocera dorsalis enhances resistance to the organophosphate insecticide trichlorphon.ResultsWe demonstrated that the gut symbiont Citrobacter sp. (CF-BD) plays a key role in the degradation of trichlorphon. Based on a comparative genomics analysis with other Citrobacter species, phosphatase hydrolase genes were identified in CF-BD. These CF-BD genes had higher expression when trichlorphon was present. Bactrocera dorsalis inoculated with isolated CF-BD obtained higher trichlorphon resistance, while antibiotic-treated flies were less resistant confirming the key role of CF-BD in insecticide resistance.ConclusionsOur findings suggest that symbiont-mediated insecticide resistance can readily develop in B. dorsalis and may represent a more widely relevant insecticide resistance mechanism than previously recognized.Electronic supplementary materialThe online version of this article (doi:10.1186/s40168-017-0236-z) contains supplementary material, which is available to authorized users.

show abstract

“…G1 (Deng et al ., ), several Pseudomonas sp . (Siripattanakul‐Ratpukdi et al ., ) and others have been isolated and reported to utilize organophosphate pesticides as their sole carbon sources. Recently, the catabolic pathway of chlorpyrifos degradation in C. nantognensis X1 T (Fang et al ., ) was reported.…”

Section: The Upper Metabolic Pathways For Degradation Of Halogenated mentioning

confidence: 99%

Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions

Pimviriyakul

Wongnate

Tinikul

et al. 2019

Microbial Biotechnology

View full text Add to dashboard Cite

Summary Halogenated aromatics are used widely in various industrial, agricultural and household applications. However, due to their stability, most of these compounds persist for a long time, leading to accumulation in the environment. Biological degradation of halogenated aromatics provides sustainable, low‐cost and environmentally friendly technologies for removing these toxicants from the environment. This minireview discusses the molecular mechanisms of the enzymatic reactions for degrading halogenated aromatics which naturally occur in various microorganisms. In general, the biodegradation process (especially for aerobic degradation) can be divided into three main steps: upper, middle and lower metabolic pathways which successively convert the toxic halogenated aromatics to common metabolites in cells. The most difficult step in the degradation of halogenated aromatics is the dehalogenation step in the middle pathway. Although a variety of enzymes are involved in the degradation of halogenated aromatics, these various pathways all share the common feature of eventually generating metabolites for utilizing in the energy‐producing metabolic pathways in cells. An in‐depth understanding of how microbes employ various enzymes in biodegradation can lead to the development of new biotechnologies via enzyme/cell/metabolic engineering or synthetic biology for sustainable biodegradation processes.

show abstract

Profenofos insecticide degradation by novel microbial consortium and isolates enriched from contaminated chili farm soil

Cited by 37 publications

References 18 publications

Biodegradation of pesticide profenofos by the free and immobilized cells ofPseudoxanthomonas suwonensisstrain HNM

Biodegradation of pesticide profenofos by the free and immobilized cells ofPseudoxanthomonas suwonensisstrain HNM

Gut symbiont enhances insecticide resistance in a significant pest, the oriental fruit fly Bactrocera dorsalis (Hendel)

Microbial degradation of halogenated aromatics: molecular mechanisms and enzymatic reactions

Contact Info

Product

Resources

About