Prof. H. B. Dixon

Fowler, Gilbert J

doi:10.1038/126958d0

Cited by 3 publications

(3 citation statements)

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“…gelsolin, which severs f-actin (Janmey et al, 1987; for review see Yin, 1987), profilin (Lassing & Lindberg, 1985) and calpactin, which binds to chromaffin granules (Drust & Creutz, 1988; for review see Glenney, 1987). Because chromaffin granules also interact with f-actin (Burridge & Phillips, 1975;Wilkins & Lin, 1981 ; Fowler & Pollard, 1982), it is possible that the PtdInsP2 or PtdInsP localizes these proteins to secretory sites and controls the interaction of chromaffin granules with the cortical cytoskeleton and the plasma membrane. Indeed, a recent report suggests that calpactin can maintain secretion in digitonin-permeabilized chromaffin cells (Ali et al, 1989).…”

Section: Atp Supports Secretion In Part By Maintaining the Polyphosphmentioning

confidence: 99%

Evidence that the inositol phospholipids are necessary for exocytosis. Loss of inositol phospholipids and inhibition of secretion in permeabilized cells caused by a bacterial phospholipase C and removal of ATP

Eberhard

Cooper

Low

et al. 1990

Biochemical Journal

216

151

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We directly manipulated the levels of PtdIns, PtdInsP and PtdInsP2 in digitonin-treated adrenal chromaffin cells with a bacterial phospholipase C (PLC) from Bacillus thuringiensis and by removal of ATP. The PtdIns-PLC acted intracellularly to cause a large decrease in [3H]inositol- or [32P]phosphate-labelled PtdIns, but did not directly hydrolyse PtdInsP or PtdInsP2. [3H]PtdInsP and [3H]PtdInsP2 levels declined markedly, probably because of the action of phosphatases in the absence of synthesis. Removal of ATP also caused marked decreases in [3H]PtdInsP and [3H]PtdInsP2. The decrease in polyphosphoinositide levels by PtdIns-PLC treatment or ATP removal was reflected by the inhibition of the production of inositol phosphates upon subsequent activation of the endogenous PLC by Ca2(+)-dependent catecholamine secretion from permeabilized cells was strongly inhibited by PtdIns-PLC treatment and by ATP removal. Ca2(+)-dependent secretion was similarly correlated with the sum of PtdInsP and PtdInsP2 when the level of these lipids was changed by either manipulation. PtdIns-PLC inhibited only the ATP-dependent component of secretion and did not affect ATP-dependent secretion. Both PtdIns-PLC and ATP removal inhibited the late slow phase of secretion, but had little effect on the initial rapid phase. Although we found a tight correlation between polyphosphoinositide levels and secretion, endogenous phospholipase C activity (stimulated by Ca2+, guanine nucleotides and related agents) was not correlated with secretion. Additional experiments indicated that neither the products of the PtdIns-PLC reaction (diacylglycerol and InsP1) nor the inability to generate products by subsequent activation of the endogenous PLC is likely to account for the inhibition of secretion. Incubation of permeabilized cells with neomycin in the absence of ATP maintained the level of polyphosphoinositides and more than doubled subsequent Ca2(+)-dependent secretion. The data suggest that: (1) Ca2(+)-dependent secretion has a requirement for the presence of inositol phospholipids; (2) the enhancement of secretion by ATP results in part from increased polyphosphoinositide levels; and (3) the role for inositol phospholipids in secretion revealed in these experiments is independent of their being substrates for the generation of diacylglycerol and InsP3.

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Section: Atp Supports Secretion In Part By Maintaining the Polyphosphmentioning

confidence: 99%

Evidence that the inositol phospholipids are necessary for exocytosis. Loss of inositol phospholipids and inhibition of secretion in permeabilized cells caused by a bacterial phospholipase C and removal of ATP

Eberhard

Cooper

Low

et al. 1990

Biochemical Journal

216

151

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“…Another advantage of these materials is their ability to be easily modified, which is primarily done to decrease potential danger of inorganic core toxicity, but these surface modification can be also done to target some biomolecules and, thus, to image biochemical pathways in vivo. Beside their successful application in in vivo imaging [2][3][4][5] and/or biology [6] in general, their applications into proteomics gain more and more attention [7][8][9][10]. Moreover, currently a great attention is paid to the targeted drug/gene delivery and the combination of therapeutic and diagnostic properties of various bioconjugates is explored, QDs are an excellent option for fluorescent labeling of numerous delivery systems.…”

Section: Introductionmentioning

confidence: 99%

Interactions between CdTe quantum dots and DNA revealed by capillary electrophoresis with laser‐induced fluorescence detection

et al. 2014

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Quantum dots (QDs) are one of the most promising nanomaterials, due to their size-dependent characteristics as well as easily controllable size during the synthesis process. They are promising label material and their interaction with biomolecules is of great interest for science. In this study, CdTe QDs were synthesized under optimal conditions for 2 nm size. Characterization and verification of QDs synthesis procedure were done by fluorimetric method and with CE. Afterwards, QDs interaction with chicken genomic DNA and 500 bpDNA fragment was observed employing CE-LIF and gel electrophoresis. Performed interaction relies on possible matching between size of QDs and major groove of the DNA, which is approximately 2.1 nm.

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“…The factors which influence the adhesion of oxide scales are not well understood. ~It has been pointed out by several investigators that small amounts of rare earth oxide dispersoids present in the alloy lead to the formation of a more adherent surface-oxide scale (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17). The literature information in this area was reviewed recently (18)(19).…”

mentioning

confidence: 99%

The Characteristics of Alumina Scales Formed on Fe‐Based Yttria‐Dispersed Alloys

Ramanarayanan

Raghavan

Petkovic-Luton

1984

J. Electrochem. Soc.

165

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To provide resistance to aggressive environments at elevated temperatures, especially in excess of -1000~ alloys or coatings which develop a-A1203 scales are the best choice. It has been pointed out that the presence of highly stable rare earth oxide dispersoids in high temperature alloys leads to improvements in the corrosion-resistant properties of A1203 scales formed on such alloys. The present study is directed toward developing an understanding of how the properties of A1203 scales formed on Fe-based alloys are influenced by yttrium oxide dispersoids in the alloy. The Fe-based alloy system selected for the current study consists of -20% Cr, -4.5% A1, -0.5% Ti, and -0.5% Y20~. The oxidation kinetics of the alloy have been established at various oxygen partial pressures in the temperature range 1000~176The a-A1203 scales which result upon oxidation are observed to be columnar, ultrafine grained, and extremely adherent when thermally stressed. Platinum markers initially placed on the alloy surface are found at the oxide/gas interface at the completion of oxidation, suggesting that scale growth occurs by exclusive inward oxygen migration. The ultrafine grain size (0.5-1 ~m) suggests that grain boundaries in the oxide scale are the preferred path for oxygen migration. The fine dispersoid particles in the alloy (200-500A) transform to coarse (-0.5 ~m) yttrium aluminum garnet upon incorporation into the AI~O.~ scale, leading to a garnet-saturated scale. It is suggested that the remarkable adherence of the ~-A1203 scales is a consequence of a combination of factors. First, yttrium doping promotes the development of a fine-grained a-A1203 scale which can effectively relieve oxide growth stresses by diffusional plastic flow. Second, because the alumina scale grows by exclusive inward oxygen transport, growth stresses arising from A1203 nucleation within an existing scale are avoided.High temperature alloys or coatings designed to resist aggressive environments at elevated temperatures should be capable of developing a surface oxide layer which is thermodynamically stable, slow growing, and adherent. The three oxides which fit the requirement of slow growth are Cr203, SiO~, and A1~O3. Of these, chromia is the fastest growing and alumina is the slowest growing. The thermodynamic stability of these oxides is in the order A12Oz > SiO2 > Cr20~. At temperatures exceeding -1000~ chromium oxide scales tend to become unstable; in environments of relatively high oxygen partial pressure, the oxide can vaporize as CrO3 (1), while under highly reducing conditions the oxide can transform to other more thermodynamically stable phases (2).For applications in hostile environments at temperatures in excess of -1000~ A1203 and SiO2 scales should be preferred to provide corrosion resistance. While the kinetic stages leading to the development of a continuous-surface oxide layer are certainly of importance, once such an oxide layer is established, the most important consideration is how well the oxide layer adheres to the alloy surface. T...

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Prof. H. B. Dixon

Cited by 3 publications

References 0 publications

Evidence that the inositol phospholipids are necessary for exocytosis. Loss of inositol phospholipids and inhibition of secretion in permeabilized cells caused by a bacterial phospholipase C and removal of ATP

Evidence that the inositol phospholipids are necessary for exocytosis. Loss of inositol phospholipids and inhibition of secretion in permeabilized cells caused by a bacterial phospholipase C and removal of ATP

Interactions between CdTe quantum dots and DNA revealed by capillary electrophoresis with laser‐induced fluorescence detection

The Characteristics of Alumina Scales Formed on Fe‐Based Yttria‐Dispersed Alloys

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