Products of acylase and β-Lactamase hydrolysis of some penicillins

Kulhánek, M.; Tadra, M.

doi:10.1007/bf02909623

Cited by 14 publications

(2 citation statements)

References 17 publications

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“…[10] We have recently demonstrated the facile immobilization of E. coli penicillin acylase as a crosslinked enzyme aggregate (CLEA), which mediated the synthesis of ampicillin in various organic solvents in the presence of 5% (v/v) of water. [11] In our continued investigations of the enzymatic synthesis of ampicillin in organic media at low water activity we turned our attention to the relatively unknown penicillin acylase from Alcaligenes faecalis [12,13] on account of its stability [13] and pH tolerance. [14] We now report the effects of the nature of the solvent and the water content on the course of the enzymatic coupling of d-PGA and 6-APA using a CLEA of A. faecalis penicillin acylase.…”

Section: Introductionmentioning

confidence: 99%

Penicillin Acylase Catalysed Synthesis of Ampicillin in Hydrophilic Organic Solvents

Langen¹,

Oosthoek²,

Rantwijk³

et al. 2003

Adv Synth Catal

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Penicillin acylase (EC 3.5.1.11) from Alcaligenes faecalis, immobilised as a cross-linked enzyme aggregate (CLEA), catalysed the synthesis of ampicillin in water-miscible organic solvents at low water concentrations. Below 4% water (v/v) no reaction was observed, showing the crucial role of water in maintaining the activity of penicillin acylase. The initial value of S/H was strongly affected by the nature of the solvent, but the effect of the water content was slight in the studied range of 4 to 15%. A reaction in acetonitrile containing 8% water afforded ampicillin in up to 86% yield.

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Section: Introductionmentioning

confidence: 99%

Penicillin Acylase Catalysed Synthesis of Ampicillin in Hydrophilic Organic Solvents

Langen¹,

Oosthoek²,

Rantwijk³

et al. 2003

Adv Synth Catal

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“…Eventualno, čestice se stabilizuju umrežavanjem primenom glutaraldehida. Postoje indikacije koje ukazuju da je industrijski biokatalizator, penicilin G hidrolaza, poznata pod komercijalnim nazivom Separase G, upravo imobilisana na sličan način, ali su detalji nedostupni [118,119].…”

Section: Imobilizacija Enzima U žElatin-hitozan Nosačeunclassified

Development of immobilized systems with penicillin acylase from Esherichia coli for production of semisyntetic penicillins

Žuža¹

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Immobilization of Penicillin G Acylase: The Key to Optimum Performance

2005

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Penicillin G acylase is a major industrial biocatalyst that is used in the enzymatic production of 20,000 t a À 1 of 6-aminopenicillanic acid, the industrial b-lactam intermediate, as well as in the enzymatic production of semi-synthetic b-lactam antibiotics. Because efficient recovery and reuse of the biocatalyst is a prerequisite for a viable process, much attention has been focused on the immobilization of penicillin G acylase. Methods that have been studied and will be discussed in this review include covalent attachment to porous organic and inorganic carriers, inclusion in and attachment to biopolymer gels and carrier-free immobilization techniques. Highly active and stable preparations have been developed; mass transfer limitations in the carrier are now a major barrier to further improvement of the biocatalyst performance.

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Products of acylase and β-Lactamase hydrolysis of some penicillins

Cited by 14 publications

References 17 publications

Penicillin Acylase Catalysed Synthesis of Ampicillin in Hydrophilic Organic Solvents

Penicillin Acylase Catalysed Synthesis of Ampicillin in Hydrophilic Organic Solvents

Development of immobilized systems with penicillin acylase from Esherichia coli for production of semisyntetic penicillins

Immobilization of Penicillin G Acylase: The Key to Optimum Performance

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