Products of 5-lipoxygenase and myeloperoxidase activities are increased in young male cigarette smokers

Loke, Wai Mun; Lam, Karen Mary-Jane; Chong, Wan Ling; Chew, Soh Eng; Quek, Amy M.L.; Lim, Erle C.H.; Seet, Raymond C.S.

doi:10.3109/10715762.2012.701291

Cited by 19 publications

(10 citation statements)

References 27 publications

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“…Alternatively, it is possible that the SCN --catalysed reduction of NO 2 -to NO in the stomach [59] was increased in the smokers, which might have attenuated NO 2 -uptake into the systemic circulation. In addition, smokers have an elevated myloperoxidase activity in neutrophils [60], which has been reported to catalyse NO 2 -oxidation [61] and might have contributed to the lower plasma [NO 2 -] after NIT in the smokers. Finally, it has been suggested that cigarette smoking impairs NO generation through eNOS, by lowering eNOS expression [62] and/or promoting eNOS uncoupling [63], and that the chemical reduction of NO 2 -can compensate for the perturbed cardiovascular function associated with NOS dysfunction [64][65][66].…”

Section: Discussionmentioning

confidence: 99%

Improvement in blood pressure after short-term inorganic nitrate supplementation is attenuated in cigarette smokers compared to non-smoking controls

et al. 2016

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Section: Discussionmentioning

confidence: 99%

Improvement in blood pressure after short-term inorganic nitrate supplementation is attenuated in cigarette smokers compared to non-smoking controls

et al. 2016

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“…The antioxidant ability of milk phenolics to inhibit neutrophil-mediated AA peroxidation was measured by the formation of F 2 -isoprostanes (stable marker of AA oxidation) quantitated by stable isotope labelled GC-MS (Mori et al 1999). Briefly, human blood neutrophils were isolated from the neutrophil/erythrocyte pellet after Ficoll-Paque gradient centrifugation and dextran sedimentation of red blood cells as previously described (Loke et al 2012). Cell viability was assessed using trypan blue exclusion and was typically >98%.…”

Section: In Vitro Antioxidant Experimentsmentioning

confidence: 99%

Cow’s milk as a dietary source of equol and phenolic antioxidants: differential distribution in the milk aqueous and lipid fractions

Tsen

Siew

Lau

et al. 2014

Dairy Sci. & Technol.

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International audienceCow’s milk contains bioactive secondary phenolic compounds that are formed by bovine’s gut bacterial flora from plant phenolic compounds. Equol, a metabolite of daidzein, is one such secondary compounds of biological interest. The potential of phenolic compounds in milk as dietary antioxidants and their distribution within the milk matrix were examined. The equol concentrations, total phenolic contents, and antioxidant activity (Trolox equivalent antioxidant capacity and inhibition of F2-isoprostanes formation by human neutrophils in vitro) were measured and compared in commercial cow’s milk of varying lipid contents. The Trolox equivalent antioxidant capacity and ability to inhibit F2-isoprostanes formation in vitro increased with the concentrations of phenolic compounds and equol in the milk. Equol and total phenolic compound concentrations correlated positively with the milk lipid concentrations. Separate experiments using homogenized and non-homogenized milk showed that dairy equol and phenolic compounds distributed to larger extent in the lipid fraction than in the aqueous fraction of cow’s milk. Our results showed that cow’s milk may serve as a dietary source of unique phenolic compounds, such as equol. Skimming of cow’s milk may reduce the original amount of equol and phenolic compounds and thereby diminishes the overall nutritive value and functional properties of cow’s milk

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“…By modulating cell surface G protein-coupled receptors (BLT1 and BLT2) [11], LTB 4 promotes migration of neutrophils, monocytes and macrophages to sites of inflammation [12]. The level of C-reactive protein (CRP) is higher in diseases in which inflammation is implicated [3,13,14]. Greater levels of CRP have been observed among dengue patients compared with age-matched non-dengue controls [3,15] and among those with greater dengue severity [15].…”

Section: Introductionmentioning

confidence: 99%

Augmentation of 5-lipoxygenase activity and expression during dengue serotype-2 infection

Loke

Chow

Sing

et al. 2013

Virol J

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BackgroundLeukotriene B4, a 5-lipoxygenase product of arachidonic acid with potent chemotactic effects on neutrophils, has not been assessed in dengue patients. In this study, plasma leukotriene B4 and serum high-sensitivity C-reactive protein levels were determined in adult patients during the febrile, convalescent and defervescent stages of dengue serotype-2 (DENV-2) infection, and compared with those of age--matched healthy and non-dengue febrile subjects. In vitro studies were performed to examine the effects of live and heat-inactivated DENV-2 on the activities and expression of 5-lipoxygenase in human neutrophils.ResultsPlasma leukotriene B4 was elevated during the febrile stages of dengue infection compared to levels during convalescence and in study controls. Plasma leukotriene B4 also correlated with serum high-sensitivity C-reactive protein in dengue patients (febrile, r = 0.91, p < 0.001; defervescence, r = 0.87, p < 0.001; convalescence, r = 0.87, p < 0.001). Exposure of human neutrophils to DENV-2 resulted in a significant rise in leukotriene B4; the extent of increase, however, did not differ between exposure to live and heat-inactivated DENV-2. Pre-incubation of either live or heat-inactivated DENV-2 resulted in reduced leukotriene B4 release by neutrophils, indicating that contact with dengue antigens (and not replication) triggers the neutrophil response. Production of leukotriene B4 was associated with an increase in 5-lipoxygenase expression in human neutrophils; addition of MK886 (a 5-lipoxygenase activating protein inhibitor) attenuated further increase in leukotriene B4 production.ConclusionThese findings provide important clinical and mechanistic data on the involvement of 5-lipoxygenase and its metabolites in dengue infection. Further studies are needed to elucidate the therapeutic implications of these findings.

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Products of 5-lipoxygenase and myeloperoxidase activities are increased in young male cigarette smokers

Cited by 19 publications

References 27 publications

Improvement in blood pressure after short-term inorganic nitrate supplementation is attenuated in cigarette smokers compared to non-smoking controls

Improvement in blood pressure after short-term inorganic nitrate supplementation is attenuated in cigarette smokers compared to non-smoking controls

Cow’s milk as a dietary source of equol and phenolic antioxidants: differential distribution in the milk aqueous and lipid fractions

Augmentation of 5-lipoxygenase activity and expression during dengue serotype-2 infection

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