Productivity of L-DOPA in in vitro shoots of Mucuna pruriens var. utilis enhanced by gamma radiation

Detmontree, Wachisunthon; Marsud, Sorrapetch; Subhadhcha, Poonsatha; Suwimol, Jetawattana; Sitthithaworn, Worapan

doi:10.7324/japs.2021.110109

Cited by 3 publications

(2 citation statements)

References 18 publications

(13 reference statements)

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“…Previous studies using γ-radiation showed that it could increase the amount of secondary metabolites in callus and shoot cultures. This was observed in the callus cultures of Lithospermum erythrorhizon [ 47 ], Rosmarinus officinalis [ 17 ], Panax ginseng [ 45 ], Nothapodytes foetida [ 48 ], Stevia rebaudiana [ 7 ], Sesuvium portulacastrum [ 49 ], Rubia cordifolia [ 43 ] and shoot cultures of Mucuna pruriens [ 50 ]. Gamma radiation could increase radical scavenging activity by induction of phenolic compounds.…”

Section: Discussionmentioning

confidence: 99%

Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)

Khalifa

Abd-ElShafy

Abu-Khudir

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background A useful technique for growing large amounts of plant material is in vitro propagation of important medicinal plants. The present investigation deals with the enhancement of secondary metabolite production via elicitation using gamma (γ)-radiation and phenylalanine (Phe) precursor feeding in callus cultures of Silybum marianum L. Results Seeds were exposed to two doses of γ-radiation (25 and 50 Gy) and the calli derived from stem explants obtained from seedlings of these radiated seeds were treated with different concentrations of Phe. The biosynthesis of phenols and flavonoids was evaluated. It was found that callus cultures derived from explants of the seeds exposed to 25 Gy γ-radiation and treated with 4 mg/l Phe accumulated the maximum phenolic content (34.27±0.02 mg/g d.wt.), while the highest flavonoid content (9.56±0.12 mg/g d.wt.) was found in callus cultures derived from explants of seeds radiated with 25 Gy γ-radiation and subjected to 1 mg/l Phe. Similarly, HPLC quantification revealed that the production of flavonoids was highly accumulated (1343.06 μg/mg d.wt.) in callus cultures from explants of seeds exposed to 25 Gy γ-radiation and grown at 1 mg/l Phe compared to the other treatments. In addition, a total of 11 important flavonoids have been determined in all callus cultures, except for acacetin-7-O-rutinoside, which was not found in the callus culture of the control. Conclusions These findings suggest that γ-radiation combined with Phe can improve the metabolism of S. marianum L. and could be used to produce such valuable metabolites on a commercial scale.

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Section: Discussionmentioning

confidence: 99%

Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)

Khalifa

Abd-ElShafy

Abu-Khudir

et al. 2022

Journal of Genetic Engineering and Biotechnology

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“…L‐DOPA showed a dose‐dependent decline, thus making it necessary to optimize the dosage to retain or eliminate anti nutritional components, which have medicinal properties too (Bhat et al, 2007). Wachisunthon et al (2021) showed that the shoots developed from velvet bean seeds germinated after treatment with 200 and 300Gy gamma radiation produced 300% and 220% higher L‐DOPA levels than nonirradiated group.…”

Section: Processing Effects On Antinutrientsmentioning

confidence: 99%

Velvet bean (Mucuna pruriens): A sustainable protein source for tomorrow

et al. 2022

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Protein is one of the essential major nutrients required for the cell functioning in human body. Complete proteins, which are usually found in animal‐based proteins, despite of having high biovailability also cause adverse effect on the environment and increased the risk of diet‐related chronic diseases. Recent years have witnessed enhanced awareness about the health benefits of substituting animal‐based proteins with plant‐based proteins, especially in developed countries. Nitrogen‐fixing grain legumes are considered important sources of protein in many developing countries as they are generally cheaper than meat or cereals. Extensive research has been conducted on several underutilized legumes with similar nutritional properties to soybean; one of these legumes is velvet beans (Mucuna pruriens). Though it is rich in protein (27%), complementary amino acid pattern to that of cereal grains exerts health beneficial properties, including antiparkinson, antidiabetic, and anticancer properties, it is limited for human consumption due to the presence of antinutrients. This review provides insight into the potential use of this underutilized legume in resolving the global protein crisis and address food insecurity issues. Additionally, the review focuses on various processing conditions necessary to utilize velvet bean for the development of food products and by‐products like protein isolate, concentrate, flour and their functional properties, and toxicological viewpoint. This current might help in driving future research and applications in velvet bean as a sustainable, plant‐based protein source for human foods along with the critical research areas for their improvement.

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Identification and characterization of stevia (Stevia rebaudiana Bert.) lines with enhanced steviol glycosides derived from gamma ray-induced in vitro mutagenesis

Subrahmanyeswari,

Gantait,

Kamble

et al. 2024

Plant Cell Tiss Organ Cult

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Productivity of L-DOPA in in vitro shoots of Mucuna pruriens var. utilis enhanced by gamma radiation

Cited by 3 publications

References 18 publications

Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)

Influence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.)

Velvet bean (Mucuna pruriens): A sustainable protein source for tomorrow

Identification and characterization of stevia (Stevia rebaudiana Bert.) lines with enhanced steviol glycosides derived from gamma ray-induced in vitro mutagenesis

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