Productivity and longevity of honey bee queens inseminated with freshly collected and diluted semen

Gerula, Dariusz; Węgrzynowicz, Paweł; Panasiuk, Beata; Bieńkowska, Małgorzata; Skowronek, W.

doi:10.1080/00218839.2016.1200880

Cited by 4 publications

(7 citation statements)

References 27 publications

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“…Our findings showed that A. cerana queen losses during the flights (i.e., 37.5% queen loss) were in line with previous reports found in A. mellifera that naturally mated queen lost about 18-40% (Palmer and Oldroyd, 2000;Medina and Goncalves, 2001;Gabka, 2018). Instrumentally inseminated queen mainly died because of injury or infection from the insemination process, or sperm residue in the oviducts (Harbo and Szabo, 1984;Collins, 2000;Chuda-Mickiewicz et al, 2009;Gerula et al, 2016). However, our result demo-nstrated that survival rate of instrumentally inseminated A. cerana queen was slightly higher than that of natural mating (Fig.…”

Section: Discussionsupporting

confidence: 90%

“…The size of the bee population where instrumentally inseminated queen was kept before and after insemination not only affected the success rate of insemination, it also affected onset of oviposition and number of sperm cells stored in the spermatheca of queen (Woyke and Jasinski, 1979;Gerula, 2007;Gerula et al, 2016). Therefore, an increase in the population of attendant worker bees increased the cluster temperature contributing to faster initiation of egg laying, and efficiency of sperm migration to store in spermatheca (Woyke and Jasiński, 1990).…”

Section: Discussionmentioning

confidence: 99%

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Impact of Confinement and Population Size on the Instrumentally Inseminated Queen’s Performance of Apis cerana Species in South Korea

Vung¹,

Kim²,

Lee³

et al. 2018

apiculture

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251Instrumental insemination of honey bee is an attractive alternative to natural mating in breeding program as it allows mating crosses between desirable queen and specific drone. However, nursery condition that the queen is kept before and after insemination is major factor affected to the performance of instrumentally inseminated queen. In this study, we evaluated the influences of three different nursery-conditions of push-in cages, mini nuclei and normal colonies on number of spermatozoa stored in the spermatheca, body weight, onset of ovipositon and performance of instrumentally inseminated Apis cerana queen. Our results demonstrated that instrumentally inseminated queens kept in mini nuclei and in normal hives showed no significant difference in queen's weight (159.8 and 166.2mg, respectively), number of spermatozoa in spermatheca (2.02 10 6 and 2.76 10 6 , respectively), proportion of queen supersedure (33.3 and 66.7% queen survival at 11 months after oviposition, respectively) and brood production, compared to naturally mated queens. In contrast, instrumentally inseminated queens kept in push-in cages showed significant difference of those above data in comparison to queens mated naturally. Our results suggested that instrumentally inseminated queens could be kept in mini nuclei containing about 1.000 attendant bees to have desirable performance of queen whereas the push-in method should be practiced for the purpose of using queen in the length of time less than 7 months. Apiculture 33(4) : 251~260 (2018) Abstract Journal of

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Impact of Confinement and Population Size on the Instrumentally Inseminated Queen’s Performance of Apis cerana Species in South Korea

Vung¹,

Kim²,

Lee³

et al. 2018

apiculture

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“…Such practices include banking queens in nursery colonies, and storing queens in cages with small number of worker bees. According to Mackensen (1969), Skowronek et al (1995), and Gerula et al (2016), dilution of semen did not significantly affect the number of spermatozoa in spermathecas of queens. All of the above studies used mixed semen, diluted in equal proportions.…”

Section: Discussionmentioning

confidence: 89%

“…We obtained 2.83 and 3.05 million, respectively. By using two semen doses of 4 μl each, Gerula et al (2016) reported the number of spermatozoa to be 4.445 and 5.126 million, respectively. In our research, after instrumental insemination, those queens kept without workers had significantly less spermatozoa in their spermathecas (2.58 million) compared to queens kept with workers (3.08 million).…”

Section: Discussionmentioning

confidence: 99%

“…Different saline diluent formulas are used and recommended (Mackensen 1969;Camargo 1975;Verma 1978;Williams and Harbo 1982;Moritz 1984;Harbo 1990; Cobey et al 2013). The effect of dilution of drone semen on the number of spermatozoa entering the spermatheca was investigated by Mackensen (1969), Skowronek et al (1995), and Gerula et al (2016). The first author used a saline solution (0.9% NaCl) or a saline solution with glucose, fructose, and trehalose (0.1%, in total), and the other authors used Hyes solution (0.9% NaCl, 0.02% CaCl 2 , 0.02% KCl, 0.01% NaHCO 3 ).…”

Section: Introductionmentioning

confidence: 99%

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Factors, based on common practices, affecting the results of instrumental insemination of honey bee queens

Gąbka¹,

Cobey²

2018

Apidologie

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This study shows how the addition of a saline solution to drone semen and the pre-and postinsemination care of honey bee queens affect both the number of sperm in the spermatheca and the condition of the oviducts. Queens were instrumentally inseminated and stored under various conditions. These conditions included being held in mailing cages with 15 or 25 workers in which all were kept at 20°C or 34°C, and being held in nursery colonies in which the queens were caged without worker bees. Some of the queens in each group were inseminated with semen only, others were inseminated with semen and saline. The number of spermatozoa in the spermatheca did not significantly differ between the queens inseminated with semen only and those inseminated with semen and the saline solution. The queens kept in mailing cages with workers had significantly more spermatozoa than those queens banked in cages without worker bees. The addition of a saline solution to semen, and the conditions the queens were stored in, did not significantly affect the condition of the oviducts. honey bee queens / instrumental insemination / number of spermatozoa in spermatheca / condition of oviducts

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Controlled reproduction in the honey bee (Apis mellifera) via artificial insemination

Gillard

Oldroyd

2020

Advances in Insect Physiology

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Productivity and longevity of honey bee queens inseminated with freshly collected and diluted semen

Cited by 4 publications

References 27 publications

Impact of Confinement and Population Size on the Instrumentally Inseminated Queen’s Performance of Apis cerana Species in South Korea

Impact of Confinement and Population Size on the Instrumentally Inseminated Queen’s Performance of Apis cerana Species in South Korea

Factors, based on common practices, affecting the results of instrumental insemination of honey bee queens

Controlled reproduction in the honey bee (Apis mellifera) via artificial insemination

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