Production System of Virus-free Apple Plants Using Heat Treatment and Shoot Tip Culture

Lee, Gunsup; Kim, Jeong Hee; Kim, Hyun Ran; Shin, Il Sheob; Cho, Kang Hee; Kim, Se Hee; Shin, Juhee; Kim, Dae Hyun

doi:10.5423/rpd.2013.19.4.288

Cited by 13 publications

(6 citation statements)

References 13 publications

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“…, (Lee, 2013). 250-400 nucleotides(nt) RNA , (Diener, 1987;Di Serio , 1996;Hashimoto Koganezawa, 1987).…”

Section: Apple Mosaic Virus Apple Chlorotic Leaf Spot Virus Apple Smentioning

confidence: 99%

Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea

Cho¹,

Kim²,

Kil³

et al. 2015

Research in Plant Disease

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To identify genome sequences of Apple scar skin viroid (ASSVd) isolates in Korea, the field survey was performed from 'Hongro' apple orchards located in eight sites in South Korea (Bongwha, Cheongsong, Dangjin, Gimchoen, Muju, Mungyeong, Suwon, and Yeongwol). ASSVd was detected by RT-PCR and PCR fragments were cloned into cloning vector. Full-length viral genomes of eight ASSVd isolates were sequenced and compared with 21 isolates reported previously from Korea, India, China, Japan and Greece. Eight isolates in this study showed 92.2-99.7% nucleotide sequence identities with those reported previously. Phylogenetic analysis showed that seven isolates reported in this study belong to the same group distinct from other groups.

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“…, (Lee, 2013). 250-400 nucleotides(nt) RNA , (Diener, 1987;Di Serio , 1996;Hashimoto Koganezawa, 1987).…”

Section: Apple Mosaic Virus Apple Chlorotic Leaf Spot Virus Apple Smentioning

confidence: 99%

Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea

Cho¹,

Kim²,

Kil³

et al. 2015

Research in Plant Disease

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“…ACLSV 30-40% 80-100% (Cembali , 2003;Nemchinov , 1995;Wu , 1998) . (Kwon , 2019;Lee , 2013;Wang , 2006), .…”

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confidence: 99%

Occurrence Status of Five Apple Virus and Viroid in Korea

Lee¹,

Cha²,

Kwon³

et al. 2020

Res. Plant Dis

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The investigation of the infection rate of domestic apple orchards by four types of apple viruses (Apple chlorotic leaf spot virus [ACLSV], Apple stem pitting virus [ASPV], Apple stem grooving virus [ASGV], Apple mosaic virus [ApMV]) and one type of viroid (Apple scar skin viroid, ASSVd) found that most apple trees were infected with viruses and viroid at the rate of 97.3%. By region, the infection rate in Jeongseon stood at 98.8%, Danyang at 100%, Yesan at 100%, Jangsu at 89.1%, and Muju at 98.1%. By each virus and viroid, the infection rate of ASGV was the highest at 93.4%, followed by ASPV at 85.7%, ACLSV at 59.0%, ASSVd at 6.7%, and ApMV at the lowest 3.6%. In addition, 84.8% of the cases were infected with two or more types of viruses and viroid, nearly seven times the single type infection rate of 12.4%, and the cases infected with three viruses, ASPV, ACLSV, and ASGV accounted for 56.2%, more than the half the total number of trees investigated.

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“…에서 ACLSV, ASPV 및 ASGV가 복합 감염되어 있었고, 3개 농원 묘목에는 ACLSV와 ASGV가 복합 감염되어 있었다고 발표한 바 있다 (Lee et al 2017). 사과나무의 경우 바이러스에 감염이 되면 소각이나 매몰 외에는 다른 방제 대책이 없기 때문에 바이러스 무병묘 생산 시스템의 개발이 필요한 실정이다 (Lee et al 2013). 사과 바이 러스 무병주 생산 방법으로는 기내 또는 기외에서 식물체를 열처리한 다음 경정배양을 병행하는 방법 (Lee et al 2013;Paprstein et al 2008), 4°C로 일정기간 처리하는 한냉요법 (El-Dougdoug et al 2010;Paprstein et al 2008;Wang et al 2006), Ribavirin과 같은 항바이러스제를 처리하는 화학적 방법 등 이 보고되었다 (Hansen and Lane 1985;Hu et al 2015).…”

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Efficient virus elimination for apple dwarfing rootstock M.9 and M.26 via thermotherapy, ribavirin and apical meristem culture

Kwon¹,

Lee²,

Kim³

et al. 2019

J Plant Biotechnol

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Apple (Malus pumila) is one of the most economically important fruits in Korea. but virus infection has decreased the sustainable production of apples and caused serious problems such as yield loss and poor fruit quality. Virus or viroid infection including apple chlorotic leaf spot virus (ACLSV), apple stem pitting virus (ASPV), apple stem grooving virus (ASGV), apple mosaic virus (ApMV) and apple scar skin viroid (ASSVd) have been also reported in Korea. In many cases, as apple gets infected with virus and viroid with no specific symptoms, the damage and symptoms caused by the viruses are not detected. In our research, viruses in the rootstock were eliminated for a virus-free apple dwarfing rootstock of M.9 and M.26. The virus elimination methods were apical meristem culture, thermotherapy (37°C, 6 weeks) and chemotherapy(Ribavirin Ⓡ). The detection of apple viruses was accomplished by Enzyme-linked Immuno-Sorbent Assay (ELlSA) and reverse transcription-polymerase chain reaction (RT-PCR). RT-PCR method was 10~30% more sensitive than the ELISA method. The efficiency of virus elimination was enhanced in apical meristem culture method. The acquisition rate of virus-free apple dwarfing rootstocks was 30~40% higher in apical meristem culture. After the meristem culturing of M.9, the infection ratio of ACLSV, ASPV and ASGV was 45%, 60% and 50%, respectively. In the apple dwarfing rootstock of M.26, the infection ratio of ACLSV, ASPV and ASGV was 40%, 55% and 55%, respectively. Based on this study, the best method for the production of virus-free apple dwarfing rootstocks was the apical meristem culture.

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Production System of Virus-free Apple Plants Using Heat Treatment and Shoot Tip Culture

Cited by 13 publications

References 13 publications

Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea

Phylogenetic Analysis of Apple scar skin viroid Isolates in Korea

Occurrence Status of Five Apple Virus and Viroid in Korea

Efficient virus elimination for apple dwarfing rootstock M.9 and M.26 via thermotherapy, ribavirin and apical meristem culture

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