Production, structure and morphology of exopolysaccharides yielded by submerged fermentation of Antrodia cinnamomea

Chen, Lei; Wang, Zhengqi; Zhang, Bobo; Ge, Mengdie; Ng, Hui-Suan; Niu, Yuge; Liu, Li

doi:10.1016/j.carbpol.2018.10.070

Cited by 28 publications

(17 citation statements)

References 31 publications

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“…The branching chains were connected with each other to form the dense structure of the polymer, which was in agreement with the observation of the EPS by SEM and AFM. Similar branched-chain morphology of the EPS from Antrodia cinnamomea as observed by TEM was reported earlier, with more flexible interchain entanglement after the addition of surfactant [35]. The microstructure of EPS was closely related to its physical properties, e.g., water binding capacity, which played an important role in maintaining the stable texture of fermented milk.…”

Section: Tem Analysissupporting

confidence: 82%

Characterization and In Vitro Fecal Microbiota Regulatory Activity of a Low-Molecular-Weight Exopolysaccharide Produced by Lactiplantibacillus plantarum NMGL2

Yao

Zhang

Lai

et al. 2022

Foods

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The exopolysaccharide (EPS) produced by Lactiplantibacillus plantarum NMGL2 isolated from traditional fermented dairy cheese was purified chromatographically with DEAE-Sepharose and Sepharose CL-6B columns. The purified EPS was characterized by various physicochemical methods and in vitro fecal microbiota regulation assay. The results showed that the EPS had a relatively low molecular weight of 3.03 × 104 Da, and it had a relatively high degradation temperature of 245 °C as determined by differential scanning calorimetry. Observation of the EPS by scanning electron microscopy, transmission electron microscopy, and atomic force microscopy revealed a highly branched and tangled fibrous network microstructure with many hollow microtubules and spherical particles. Structural study by 1H NMR spectroscopy suggested that the EPS contained a tetrasaccharide repeating unit with monosaccharide components of β-galactose (4.6%), α-glucose (20.6%), and α-mannose (74.8%). The EPS was highly resistant to hydrolysis of simulated human saliva, gastric, and intestinal juices. Moreover, the EPS beneficially affected the composition and diversity of the fecal microbiota, e.g., increasing the relative abundance of Firmicutes and inhibiting that of Proteobacteria. The results of this study indicated significant bioactivity of this novel low-molecular-weight EPS produced by Lpb. plantarum NMGL2, which could serve as a bioactive agent for potential applications in the food and health care industry.

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Section: Tem Analysissupporting

confidence: 82%

Characterization and In Vitro Fecal Microbiota Regulatory Activity of a Low-Molecular-Weight Exopolysaccharide Produced by Lactiplantibacillus plantarum NMGL2

Yao

Zhang

Lai

et al. 2022

Foods

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“…Hence, we viewed lactose as the best carbon source for the EPS production in A. biennis. This finding was opposite to the results for the carbon source requirements reported by other researchers in different fungi or mushrooms (Chen et al 2019;Meng et al 2010). Figure 1(B) shows that among the six different nitrogen sources, organic nitrogen sources produced the relatively highest mycelial growth and EPS production in A. biennis.…”

Section: Effects Of Carbon and Nitrogen Sources For Eps Productioncontrasting

confidence: 74%

“…Exopolysaccharides (EPSs) as valuable metabolites have also been secreted in large quantities into the culture broth of A. biennis. Many studies reported that the EPSs harvested from fermentation broths of various fungi or mushrooms exhibit notable and excellent bioactivities, such as immunostimulatory, antitumor and antioxidant activities, and other pharmacological functions, thereby having great potential for applications in food, medicine and cosmetics (Cao et al 2014;Chen et al 2019;He et al 2012;Meng et al 2010). To the best of our knowledge, no or little information is available on the isolation, characterization and bioactivities of EPS produced from A. biennis in submerged fermentation.…”

Section: Introductionmentioning

confidence: 99%

Preliminary Characterization of Exopolysaccharides Produced by Abortiporus biennis in Submerged Fermentation

Zhang¹,

Liu²,

Li³

et al. 2019

JSM

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Abortiporus biennis is a rare edible and medicinal mushroom that is valuable in traditional Chinese medicine. Exopolysaccharides (EPSs) as valuable metabolites have been harvested from fermentation broths of A. biennis. However, studies on preliminary characterisations and bioactivities of A. biennis EPSs are lacking. In this study, therefore, the production, characteristics and bioactivity of EPSs produced by A. biennis in submerged fermentation were investigated. An EPS-1 was isolated and purified from the culture broth of A. biennis using a Sepharose CL-6B column chromatography. The molecular weight, monosaccharide composition, structural characteristics, chain conformation and thermal property of EPS-1 were determined by gel-filtration chromatography, gas chromatography-mass spectroscopy, Fourier-transform infrared spectroscopy, Congo red test and thermogravimetric analysis, respectively. The in vitro antioxidant and immunoregulation activities of the EPS-1 were evaluated by adopting DPPH and hydroxyl radical-scavenging assays, and analysing NO production in macrophage RAW 264.7 cells, respectively. Results showed that the culture medium containing 30 g/L lactose and 3.0 g/L tryptone had the most suitable carbon and nitrogen sources, respectively, for EPS production in A. biennis. The maximum EPS production (18.29 g/L) was obtained after 6 days of cultivation in a 5 L stirred-tank reactor under the most suitable culture medium. After purification, EPS-1 with 85.4% yield and 96.1% carbohydrate content was obtained from the culture broth of A. biennis. EPS-1 was characterised as a neutral heteropolysaccharide with a molecular weight of 22.07 kDa and is composed of glucose, mannose and galactose at a molar ratio of 3.3:2.0:1.0. EPS-1 with a relatively high thermal stability existed as a random coil conformation in the aqueous medium and possessed prominent radical-scavenging ability and macrophage stimulation activity in vitro. Therefore, EPS-1 could be explored as a functional ingredient with potential applications in food, medical and cosmetic industries.

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“…3 B. It’s worth noting that Angelica sinensis polysaccharide (ASP) has a high affinity for asialoglycoprotein receptor (ASGPR), which can accomplish active targeting without adding ligands. The higher galactose content, branching structure, and suitable spatial geometry may contribute to the affinity [ 52 – 54 ]. ASP is water-soluble polysaccharides, and it is mainly composed of glucose (Glc), galactose (Gal), arabinose (Ara), rhamnose (Rha), fucose (Fuc), xylose (Xyl) and galacturonic acid (GalUA).…”

Section: Targetingmentioning

confidence: 99%

A review on the applications of Traditional Chinese medicine polysaccharides in drug delivery systems

Wang

Xiong

et al. 2022

Chin Med

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Traditional Chinese medicine polysaccharides (TCMPs) are plentiful and renewable resources with properties such as biocompatibility, hydrophilicity, biodegradability, and low cytotoxicity. Because the polysaccharide molecular chain contains a variety of active groups, different polysaccharide derivatives can be easily produced through chemical modification. They have been increasingly used in drug delivery systems (DDS). However, the potential of polysaccharides is usually ignored due to their structural complexity, poor stability or ambiguity of mechanisms of actions. This review summarized the applications of TCMPs in DDS around four main aspects. The general characteristics of TCMPs as drug delivery carriers, as well as the relationships between structure and function of them were summarized. Meanwhile, the direction of preparing multifunctional drug delivery materials with synergistic effect by using TCMPs was discussed. This review aims to become a reference for further research of TCMPs and their derivatives, especially applications of them as carriers in pharmaceutical preparation industry.

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Production, structure and morphology of exopolysaccharides yielded by submerged fermentation of Antrodia cinnamomea

Cited by 28 publications

References 31 publications

Characterization and In Vitro Fecal Microbiota Regulatory Activity of a Low-Molecular-Weight Exopolysaccharide Produced by Lactiplantibacillus plantarum NMGL2

Characterization and In Vitro Fecal Microbiota Regulatory Activity of a Low-Molecular-Weight Exopolysaccharide Produced by Lactiplantibacillus plantarum NMGL2

Preliminary Characterization of Exopolysaccharides Produced by Abortiporus biennis in Submerged Fermentation

A review on the applications of Traditional Chinese medicine polysaccharides in drug delivery systems

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