Production, purification and therapeutic potential of egg yolk antibodies for treating Trypanosoma evansi infection

Sampaio, Luzia Cristina Lencioni; Baldissera, Matheus D.; Grando, Thirssa H.; Gressler, Lucas T.; Capeleto, Dianni de Menezes; de, Mariângela Facco; Jesus, Francielli Pantella Kuns de; Santos, Alceu Gonçalves dos; Anciuti, Andréia Nobre; Colonetti, Karina; Stainki, Daniel Roulim; Monteiro, Sílvia González

doi:10.1016/j.vetpar.2014.05.032

Cited by 21 publications

(19 citation statements)

References 35 publications

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“…Sampaio et al produced a high avidity IgY against the protozoan Trypanosoma evansi. There was neither prophylactic action nor infection control in the treated rats, but anti-T. evansi IgY increased their survival rate when used concomitantly to anti-hematozoa drugs [86]. In another study, Grando et al inoculated Trypanosoma cruzi trypomastigots in hens and extracted a high amount of anti-T. cruzi IgY that cross-reacted with T. evansi antigens in Western blot, showing that the anti-T. cruzi IgY is not reliable as a diagnostic tool, but deserves more investigations as a possible therapeutic resource for trypanosomes infection [5].…”

Section: Antiparasitic Activitymentioning

confidence: 90%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

124

135

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A B S T R A C TEgg yolk constitutes a relevant alternative source of antibodies. It presents some advantages over mammalian serum immunoglobulins regarding productivity, animal welfare and specificity. The main immunoglobulin present in avian blood (IgY) is transmitted to their offspring and accumulates in egg yolks, which enables the non-invasive harvesting of high amounts of antibodies. Moreover, due to structural differences and phylogenetic distance, IgY is more suitable for diagnostic purposes than mammalian antibodies, since it does not react with certain components of the human immune system and displays greater avidity for mammalian conserved proteins. IgY has been extensively used in health researches, as both therapeutic and diagnostic tool. This article aims to review its applications in both human and veterinary health.

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Section: Antiparasitic Activitymentioning

confidence: 90%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

124

135

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“…Trypomastigotes forms of T. cruzi (strain Y) was used as antigen to immunize the chickens, according the method described by Sampaio et al (2014b), with some adaptations for T. cruzi, as follows below: cryopreserved trypomastigotes were inoculated into mice for reactivation and multiplication of strain, and blood samples were collected at the peak of parasitemia. In order to separate the parasites, blood was diluted in DMEM medium (1:1 v/v), followed by centrifugation (400 g for 10 min).…”

Section: The Antigenmentioning

confidence: 99%

“…The chicken immunization protocol was as described by Sampaio et al (2014b). Briefly, a solution containing trypomastigotes of T. cruzi was added to incomplete Freund's adjuvant (Difco®) at a 1:1 ratio, with a final volume of 1.0 mL.…”

Section: Chicken Immunization Protocolmentioning

confidence: 99%

“…The Enzyme Linked Immune Sorbent Assay (ELISA) was performed as described by Sampaio et al (2014b). A 96-well ELISA plate was coated with T. cruzi antigen (1 μg/well) previously diluted in 0.05 M of carbonate buffer (pH 9.6).…”

Section: Elisamentioning

confidence: 99%

“…In addition, IgY shows numerous advantages when compared to mammalian antibodies, such as: similar functions of the mammalian IgG and IgE, no cross reaction with mammalian IgG, do not attack red blood cells, do not activate the complement system and the coagulation cascade. Furthermore, chickens are more able to produce antibodies than rabbits, goats, horses or rodents (Sampaio et al, 2014b;Chacana et al, 2004;Contreras et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

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Avian antibodies (IgY) against Trypanosoma cruzi : Purification and characterization studies

Grando

Baldissera

Sá

et al. 2017

Journal of Immunological Methods

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Trypanosoma cruzi is a flagellated protozoan belonging to the Trypanosomatidae family, the etiologic agent of Chagas disease. Currently, there is neither a licensed vaccine nor effective treatment, characterizing an unmet clinical need. The IgY refers to the egg yolk immunoglobulin (Y=yolk) and its production and use are subjects of many studies due to the diversity of its diagnostic and therapeutic applications. Several researchers have shown that the use of specific IgY may prevent and/or control infectious and parasitic diseases. Based on these evidences, the aim of this study was to immunize chickens with trypomastigotes of T. cruzi in order to produce highly effective and pure antibodies (IgY), as well as extract, characterize, quantify, and verify cytotoxic effects of IgY anti-T. cruzi. After the induction of IgY production by chickens, the eggs were collected and the IgY was extracted by method of precipitation of polyethylene glycol 6000. The IgY anti-T. cruzi characterization was performed using polyacrylamide gel electrophoresis (SDS-PAGE), western-blot and enzyme-linked immunosorbent assay (ELISA). Moreover, the cytotoxic or proliferative effects of IgY anti-T. cruzi was verified by MTT assay. The concentration of IgY in yolk was 8.41±1.47mg/mL. The characterization of IgY reveled bands of stained peptides with molecular weight between 75 and 50kDa and 37 and 25kDa. In the ELISA test was observed that there was antigen-antibody reaction throughout the sample period. The concentrations of 1, 5 and 10mg/mL of IgY anti-T. cruzi presented no cytotoxic of proliferative effects in mononuclear and VERO cells in vitro. The results indicated that T. cruzi is able to generate a high production of specific immunoglobulins in chickens, it did not cause damage to the cell membrane and no proliferative effect.

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Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom

Leiva

Cangelosi

Mariconda

et al. 2023

Appl Microbiol Biotechnol

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Production, purification and therapeutic potential of egg yolk antibodies for treating Trypanosoma evansi infection

Cited by 21 publications

References 35 publications

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Avian antibodies (IgY) against Trypanosoma cruzi : Purification and characterization studies

Use of adjuvant ISA VG 71 to produce neutralizing egg yolk antibodies against bothropic venom

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