Production, purification and chemical characterization of the catecholate siderophore from potent probiotic strains of Bacillus spp.

Patel, Anil Kumar; Deshattiwar, Maroti K.; Chaudhari, Bhushan L.; Chincholkar, S. B.

doi:10.1016/j.biortech.2008.05.008

Cited by 70 publications

(56 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar methods were used previously to purify catecholate type of siderophore from P. fluorescens [39] and strains of Bacillus spp. [55] followed by NMR studies for determination of siderophore structure.…”

Section: Resultsmentioning

confidence: 99%

Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32

et al. 2014

View full text Add to dashboard Cite

Acinetobacter calcoaceticus HIRFA32 from wheat rhizosphere produced catecholate type of siderophore with optimum siderophore (ca. 92 % siderophore units) in succinic acid medium without FeSO 4 at 28°C and 24 h of incubation. HPLC purified siderophore appeared as pale yellow crystals with molecular weight [M C NMR, HMQC, HMBC, NOESY and decoupling studies, revealed that siderophore composed of 2,3-dihydroxybenzoic acid with hydroxyhistamine and threonine as amino acid subunits. In vitro study demonstrated siderophore mediated mycelium growth inhibition (ca. 46.87 ± 0.5 %) of Fusarium oxysporum. This study accounts to first report on biosynthesis of acinetobactin-like siderophore by the rhizospheric strain of A. calcoaceticus and its significance in inhibition of F. oxysporum.

show abstract

Section: Resultsmentioning

confidence: 99%

Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Hemolytic activity is a highly recommended test by EFSA to ensure that the bacterial strain was free of the toxigenic potential [74]. Although studies on fish and pigs reported that the hemolytic activity of microbial enzymes in vitro does not necessarily produce any negative effect in vivo [75] [76], hemolytically active bacteria are not recommended as feed additives according to EFSA guidelines [71]. Based on our results, we propose the evaluation of bacterial hemolytic activity accompanied by the assessment of fibrinolytic activity.…”

Section: Discussionmentioning

confidence: 99%

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895

Algburi¹,

Volski²,

Cugini³

et al. 2016

AiM

View full text Add to dashboard Cite

This study reports on the safety and putative probiotic properties of Bacillus amyloliquefaciens B-1895 and Bacillus subtilis KATMIRA1933. According to the bacterial reverse mutation (Ames) test, cell-free supernatants of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were not mutagenic. The two strains co-aggregated with Escherichia coli and Pseudomonas aeruginosa, and cell-free supernatants inhibited the growth of Streptococcus intermedius and Porphyromonas gingivalis. Endospores of B. amyloliquefaciens B-1895 and B. subtilis KATMIRA1933 were tolerant to 0.3% (w/v) bile salts and survived incubation for 4 h in MRS broth at pH 2.0 to 3.0. The ability of the two strains to produce antimicrobial compounds potentiates their application in health care formulations, personal care products, food and animal feed.

show abstract

“…Lyophilized siderophore sample was pelleted with potassium bromide (KBr) and subjected to FTIR spectroscopy (Jasco 460 plus) for determination of functional groups. Spectra were recorded in the range from 4,000 to 400 cm -1 [26].…”

Section: Siderophore Quantification In Different Mediamentioning

confidence: 99%

Chemical and structural characterization of hydroxamate siderophore produced by marine Vibrio harveyi

Murugappan

Aravinth

Karthikeyan³

2010

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

In the present study, 22 different bacteria were isolated from open ocean water from the Gulf of Mannar, India. Of the 22 isolates, 4 were identified as Vibrio spp. (VM1, VM2, VM3 and VM4) and found to produce siderophores (iron-binding chelators) under iron-limited conditions. Different media were found to have an influence on siderophore production. Maximum siderophore production was observed with VM1 isolate in MM9 salts medium at 48 h of incubation. The isolate was confirmed as Vibrio harveyi based on 16S rRNA gene sequencing and phylogenetic analysis. Fourier-transform infrared (FTIR) and (1)H nuclear magnetic resonance (NMR) spectra revealed the hydroxamate nature of the siderophore produced. Further characterization of the siderophore revealed it to be of dihydroxamate nature, forming hexadentate ligands with Fe(III) ions. A narrow shift in ultraviolet (UV)-Vis spectrum was observed on photolysis due to ligand oxidation. Growth-promotion bioassay with Aeromonas hydrophila, Staphylococcus aureus and E. coli confirmed the iron-scavenging property of the siderophore produced by Vibrio harveyi.

show abstract

Production, purification and chemical characterization of the catecholate siderophore from potent probiotic strains of Bacillus spp.

Cited by 70 publications

References 31 publications

Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32

Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895

Chemical and structural characterization of hydroxamate siderophore produced by marine Vibrio harveyi

Contact Info

Product

Resources

About

Production, purification and chemical characterization of the catecholate siderophore from potent probiotic strains of Bacillus spp.

Cited by 70 publications

References 31 publications

Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32

Characterization and Fungal Inhibition Activity of Siderophore from Wheat Rhizosphere Associated Acinetobacter calcoaceticus Strain HIRFA32

Safety Properties and Probiotic Potential of &lt;i&gt;Bacillus subtilis&lt;/i&gt; KATMIRA1933 and &lt;i&gt;Bacillus amyloliquefaciens&lt;/i&gt; B-1895

Chemical and structural characterization of hydroxamate siderophore produced by marine Vibrio harveyi

Contact Info

Product

Resources

About

Safety Properties and Probiotic Potential of <i>Bacillus subtilis</i> KATMIRA1933 and <i>Bacillus amyloliquefaciens</i> B-1895