Production, purification and characterization of hemolysins from Listeria ivanovii and Listeria monocytogenes Sv4b

Kreft, Jürgen

doi:10.1016/0378-1097(89)90298-x

Cited by 10 publications

(18 citation statements)

References 7 publications

(7 reference statements)

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“…Ivanolysin (ILO) and listeriolysin (LLO) are closely related extracellular proteins produced by L. ivanovii and L. monocytogenes (23,39). These haemolysins belong to the sulfhydryl (SH)-activated group of bacterial toxins expressed by diverse species of grampositive bacteria and are the only thiol-activated toxins produced by intracellular bacteria (22,40).…”

Section: Discussionmentioning

confidence: 99%

Invasive Pathway ofListeria Ivanoviiin Human Amnion-Derived Wish Cells

Ammendolia¹,

Superti²,

Bertuccini³

et al. 2007

Int J Immunopathol Pharmacol

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Among Listeria genus, only two species, Listeria ivanovii and Listeria monocytogenes, are pathogenic. L. ivanovii is almost only associated with infections in animals, mainly sheep and cattle, and has rarely been associated with human infections, whereas L. monocytogenes causes severe illnesses in both humans and animals. To further investigate the pathogenetic features of L. ivanovii in humans, we undertook a study in which the intracellular behaviour of this pathogen was analysed in WISH cells, a cell line derived from human amniotic tissue, and compared to that of L. monocytogenes. Using microbiological, biochemical, and ultrastructural approaches, we demonstrate that L. ivanovii can adhere to and invade human amniotic cells, lyse the phagosomal membrane, polymerize host cell actin, and spread from cell to cell more efficiently than L. monocytogenes. However, although L. ivanovii is capable of specifically infecting and replicating in human amnion cells, its survival in cytoplasm is limited compared to that of L. monocytogenes.

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Section: Discussionmentioning

confidence: 99%

Invasive Pathway ofListeria Ivanoviiin Human Amnion-Derived Wish Cells

Ammendolia¹,

Superti²,

Bertuccini³

et al. 2007

Int J Immunopathol Pharmacol

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“…We have reported that LLO, the principal virulence factor of L. monocytogenes, plays a pivotal role in the generation of host resistance by induction of Th1 cytokines such as IL-12, IL-18, and IFN-␥ (29,30,36). L. ivanovii is known to secrete ILO, which is highly homologous to LLO (20,40). They have similar signal sequences (25 amino acids in LLO and 24 amino acids in ILO), and the secreted form of both proteins is 504 amino acids long (Fig.…”

Section: Ifn-␥-inducing Ability Of L Monocytogenes and L Ivanoviimentioning

confidence: 99%

“…Both listeriolysin O (LLO), an hly gene product in L. monocytogenes, and ivanolysin O (ILO), an ilo gene product in L. ivanovii, are 58-kDa secretory proteins that are the major virulence determinants in each (12,20,40). They show 80% homology in amino acid sequence (14,16) and belong to a family of cholesterol-dependent cytolysins (CDCs) characterized by the presence of a highly conserved undecapeptide sequence (ECTGLAWEWWR) located near the C terminus (7,42).…”

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Differences in Gamma Interferon Production Induced by Listeriolysin O and Ivanolysin O Result in Different Levels of Protective Immunity in Mice Infected with Listeria monocytogenes and Listeria ivanovii

et al. 2003

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Two pathogenic species in the genus Listeria, Listeria monocytogenes and Listeria ivanovii, are characterized by the production of hemolysins belonging to cholesterol-dependent cytolysins, listeriolysin O (LLO) and ivanolysin O (ILO), respectively. LLO, produced by L. monocytogenes, is able to induce gamma interferon (IFN-␥) production and contributes to the generation of Th1-dependent protective immunity. On the other hand, nothing is known about the role of ILO, produced by L. ivanovii, in this regard. In this study, we immunized mice with 0.1 50% lethal dose (LD 50 ) of L. monocytogenes and L. ivanovii. Protective immunity against a challenge with 10 LD 50 was generated in mice infected with L. monocytogenes, whereas L. ivanovii infection did not induce protection. After immunization, the level of IFN-␥ in serum samples was increased in mice given L. monocytogenes but not in those given L. ivanovii. To determine the IFN-␥-inducing activity of cytolysins, recombinant protein was constructed. Recombinant ILO exhibited significantly lower IFN-␥-inducing activity than LLO. By comparing the IFN-␥-inducing activity of a chimera incorporating LLO and ILO, it was found that domains 1 to 3 of LLO were critical for IFN-␥-inducing activity while the counterpart in ILO was unable to induce cytokine production. These results suggested that the weak ability of ILO to induce IFN-␥ production is responsible for the failure of L. ivanovii to generate effective protective immunity.Among the bacterial species belonging to the genus Listeria, two species, Listeria monocytogenes and Listeria ivanovii, are known to be pathogenic (42). L. monocytogenes causes serious infections in newborns, pregnant women, immunocompromised individuals (9,11,24), and animals (25). L. ivanovii is pathogenic to ruminants (25) but rarely causes human disease (6, 23).These two species possess a similar central virulence gene cluster consisting of prfA, plcA, hly, mpl, actA, and plcB, the transcription of which is positively regulated by PrfA (13, 21). Both listeriolysin O (LLO), an hly gene product in L. monocytogenes, and ivanolysin O (ILO), an ilo gene product in L. ivanovii, are 58-kDa secretory proteins that are the major virulence determinants in each (12,20,40). They show 80% homology in amino acid sequence (14, 16) and belong to a family of cholesterol-dependent cytolysins (CDCs) characterized by the presence of a highly conserved undecapeptide sequence (ECTGLAWEWWR) located near the C terminus (7, 42). The CDCs are known to bind cholesterol on the cell surface and form oligomers, resulting in a ring-shaped pore on the cytoplasmic membrane. The cytolytic activity of CDC is easily blocked by treatment with small amounts of free cholesterol (2). Rossjohn et al. analyzed the three-dimensional structure of perfringolysin O, a member of the CDC family produced by Clostridium perfringens, and reported that perfringolysin O consists of four domains (34). The continuous domains 1 to 3 are involved in oligomerization and insertion of the oligomer into th...

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“…These toxins include the species-specific types of Iisteriolysin 0 produced by the three Listeria species which are hemolytic on blood agar: Listeria monocytogenes (pathogenic for man anct animals), Listeria ivanoL·ii (animal pathogen) and Listeria seeligeri (apathogenic). Their Iisteriolysins have previously bet!n characterized biochemically [6,10],and it has also been shown that they vary in cytolytic activity.The gene for Iisteriolysin 0 (h/y) from 1.4. monocytogenes has been cloned and sequenced [4,13] and homologous DNA sequences have been detected in the chromosome of the two other hemolytic Listeria species [11]. lt has been firmly established, that Iisteriolysin 0 (LLO) is an essential virulence factor of L. monocytogenes (reviewed in Refs.…”

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